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zs78g11.s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:703652 3', mRNA sequence

GenBank: AA278626.1

FASTA Graphics 

LOCUS       AA278626                 494 bp    mRNA    linear   EST 08-JAN-2011
DEFINITION  zs78g11.s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:703652 3',
            mRNA sequence.
ACCESSION   AA278626
VERSION     AA278626.1
DBLINK      BioSample: SAMN00155198
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 494)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 817   Std Error: 0.00
            Seq primer: -41m13 fwd. ET from Amersham
            High quality sequence stop: 471.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:703652"
                     /tissue_type="germinal center B cell"
                     /clone_lib="SAMN00155198 NCI_CGAP_GCB1"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was prepared from human tonsillar cells
                     enriched for germinal center B cells by flow sorting
                     (CD20+, IgD-), provided by Dr. Louis M. Staudt (NCI), Dr.
                     David Allman (NCI) and Dr. Gerald Marti (CBER). cDNA
                     synthesis was primed with a Not I - oligo(dT) primer
                     [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCTCATTTTTTTTTTTTTTTTTT-
                     3']. Double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization, and was
                     constructed by Bento Soares and M. Fatima Bonaldo."
ORIGIN      
        1 agtaattctt atggtattgc tgggtctctc aggaatatgt atcatttgat tttgagcatg
       61 tggggttaag gtattagatt actaccacaa accgtagacc cctgcatggc accacattta
      121 ttttcaggag tagatgttac atggcaggta tcaaaatgtg atgatcaatt ctgtgttttc
      181 tgttgattaa acctcctcat ttggttaaat agccagtgta taccagagag ttctacacag
      241 gtcaaaatta tatcactttt agagcagcaa tacgtgatcc aaggttttct tgaaggtagt
      301 gcaaaagatg cagttcataa tgttctcccg attcaggaac tcttatgctg tgtctctcct
      361 gaggatagat ctgtaaatca tatggctttc cagccctcac taaaaaactc agtaatatac
      421 tggtatgtgc aaatggacat tctcatccag gaaaccatgt aagagcagta aacgatttgg
      481 ttcagagggg aact
//
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