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GenBank: AA283881.1
FASTA Graphics
LOCUS AA283881 285 bp mRNA linear EST 05-JAN-2011 DEFINITION zs47g05.s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:700664 3', mRNA sequence. ACCESSION AA283881 VERSION AA283881.1 DBLINK BioSample: SAMN00155198 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 285) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Insert Length: 1184 Std Error: 0.00 Seq primer: -41m13 fwd. ET from Amersham High quality sequence stop: 277. FEATURES Location/Qualifiers source 1..285 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="GDB:5740094" /db_xref="taxon:9606" /clone="IMAGE:700664" /tissue_type="germinal center B cell" /clone_lib="SAMN00155198 NCI_CGAP_GCB1" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was prepared from human tonsillar cells enriched for germinal center B cells by flow sorting (CD20+, IgD-), provided by Dr. Louis M. Staudt (NCI), Dr. David Allman (NCI) and Dr. Gerald Marti (CBER). cDNA synthesis was primed with a Not I - oligo(dT) primer [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCTCATTTTTTTTTTTTTTTTTT- 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo." ORIGIN 1 atcattaact ctagaatcat ccttcaggaa tgacaaatca ttccacactt gaacaactgg 61 ttttcacata acagcctaag aaaagagcac ctggaggaaa cagcgtgaca agaagactga 121 cagtgaggtc agtggcaaaa gagtaatgca aaggtgtaat gtggctacaa cgagaacgtg 181 agctgtgggt gttttgccag aacatggagt gcgctgaaag gaatgacacc aacacagggg 241 cagtaaagat gggcacagtt tgacctcagg agacaaaaat tttcc //
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