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zx05e02.s1 Soares_total_fetus_Nb2HF8_9w Homo sapiens cDNA clone IMAGE:785594 3', mRNA sequence

GenBank: AA449448.1

FASTA Graphics 

LOCUS       AA449448                 312 bp    mRNA    linear   EST 04-JUN-1997
DEFINITION  zx05e02.s1 Soares_total_fetus_Nb2HF8_9w Homo sapiens cDNA clone
            IMAGE:785594 3', mRNA sequence.
ACCESSION   AA449448
VERSION     AA449448.1
DBLINK      BioSample: SAMN00155442
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S.,
            Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin,J.,
            Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B.,
            White,Y., Wylie,T., Waterston,R. and Wilson,R.
  TITLE     WashU-Merck EST Project 1997
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: -41m13 fwd. ET from Amersham
            High quality sequence stop: 288.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:5983212"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:785594"
                     /clone_lib="SAMN00155442 Soares_total_fetus_Nb2HF8_9w"
                     /dev_stage="8-9 weeks"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was prepared from mRNA obtained from
                     pooled 8-9 week (total) fetus material with a Not I -
                     oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCTTAATTTTTTTTTTTTTTTTT 3'].
                     Double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization, and was
                     constructed by Bento Soares and M. Fatima Bonaldo."
ORIGIN      
        1 tttttttctg tacgtatgtt ttatttgaac atccacattt cttagcttag agacaattat
       61 attccgtaca cttttcttct ctctctctta caatatttag actggccaaa aaccagggaa
      121 aatgtccttg gactgcagtc attataaaat tttacttaat gcttatgaaa gcactcatgt
      181 gaaaagcttc agcatgaagt gtaatcacca cattcagttt caaagttcaa atgcccattc
      241 ctatgatggg taaacaccta ccatagcgca aagaaggaga gtgattgtgg gtaatgacag
      301 aggaggaaaa gt
//
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