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GenBank: AA489080.1
FASTA Graphics
LOCUS AA489080 344 bp mRNA linear EST 04-JAN-2011 DEFINITION aa54h08.s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:824799 3', mRNA sequence. ACCESSION AA489080 VERSION AA489080.1 DBLINK BioSample: SAMN00155198 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 344) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Louis M. Staudt, M.D., Ph.D., David Allman, Ph.D., Gerald Marti, M.D. cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima Bonaldo, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Seq primer: -41m13 fwd. ET from Amersham. FEATURES Location/Qualifiers source 1..344 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:824799" /tissue_type="germinal center B cell" /clone_lib="SAMN00155198 NCI_CGAP_GCB1" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was prepared from human tonsillar cells enriched for germinal center B cells by flow sorting (CD20+, IgD-), provided by Dr. Louis M. Staudt (NCI), Dr. David Allman (NCI) and Dr. Gerald Marti (CBER). cDNA synthesis was primed with a Not I - oligo(dT) primer [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCTCATTTTTTTTTTTTTTTTTT- 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo." ORIGIN 1 tttttttttt tttttttttt tttttggtag ttcttagttt tattataacc ttgtattttc 61 tggcaaaaat ataaatctaa atgcatgatc tctgggcaca cagctcaagt atcagccttg 121 agatgaccta agcagcaaaa atttggccta tttaattaaa tgcacaggag gttgcagccg 181 catttattag aaaaatatta tcctttggaa attcctttct tgaagattgg ctccagggcg 241 ttgttctttc tgtttttatg caattgcact tccttggcag gcagccaggc gctccggtgc 301 tcacaggcca tgggacactc cagttccctg cagacccagc gggc //
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