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aa54h08.s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:824799 3', mRNA sequence

GenBank: AA489080.1

FASTA Graphics 

LOCUS       AA489080                 344 bp    mRNA    linear   EST 04-JAN-2011
DEFINITION  aa54h08.s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:824799 3',
            mRNA sequence.
ACCESSION   AA489080
VERSION     AA489080.1
DBLINK      BioSample: SAMN00155198
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 344)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: Louis M. Staudt, M.D., Ph.D., David Allman,
            Ph.D., Gerald Marti, M.D.
             cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima
            Bonaldo, Ph.D.
             cDNA Library Arrayed by: Greg Lennon, Ph.D.
             DNA Sequencing by: Washington University Genome Sequencing Center
             Clone distribution: NCI-CGAP clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            www-bio.llnl.gov/bbrp/image/image.html
            
            Seq primer: -41m13 fwd. ET from Amersham.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:824799"
                     /tissue_type="germinal center B cell"
                     /clone_lib="SAMN00155198 NCI_CGAP_GCB1"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was prepared from human tonsillar cells
                     enriched for germinal center B cells by flow sorting
                     (CD20+, IgD-), provided by Dr. Louis M. Staudt (NCI), Dr.
                     David Allman (NCI) and Dr. Gerald Marti (CBER). cDNA
                     synthesis was primed with a Not I - oligo(dT) primer
                     [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCTCATTTTTTTTTTTTTTTTTT-
                     3']. Double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization, and was
                     constructed by Bento Soares and M. Fatima Bonaldo."
ORIGIN      
        1 tttttttttt tttttttttt tttttggtag ttcttagttt tattataacc ttgtattttc
       61 tggcaaaaat ataaatctaa atgcatgatc tctgggcaca cagctcaagt atcagccttg
      121 agatgaccta agcagcaaaa atttggccta tttaattaaa tgcacaggag gttgcagccg
      181 catttattag aaaaatatta tcctttggaa attcctttct tgaagattgg ctccagggcg
      241 ttgttctttc tgtttttatg caattgcact tccttggcag gcagccaggc gctccggtgc
      301 tcacaggcca tgggacactc cagttccctg cagacccagc gggc
//
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