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om40d09.s1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE:1543505 3', mRNA sequence

GenBank: AA921816.1

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LOCUS       AA921816                 503 bp    mRNA    linear   EST 08-JAN-2011
DEFINITION  om40d09.s1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone
            IMAGE:1543505 3', mRNA sequence.
ACCESSION   AA921816
VERSION     AA921816.1
DBLINK      BioSample: SAMN00155629
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 503)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 810   Std Error: 0.00
            Seq primer: -40m13 fwd. ET from Amersham
            High quality sequence stop: 375.
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:1543505"
                     /clone_lib="SAMN00155629 Soares_NFL_T_GBC_S1"
                     /lab_host="DH10B"
                     /note="Organ: pooled; Vector: pT7T3D-PacI; Site_1: Not I;
                     Site_2: Eco RI; Equal amounts of plasmid DNA from three
                     normalized libraries (fetal lung NbHL19W, testis NHT, and
                     B-cell NCI_CGAP_GCB1) were mixed, and ss circles were made
                     in vitro. Following HAP purification, this DNA was used as
                     tracer in a subtractive hybridization reaction. The driver
                     was PCR-amplified cDNAs from pools of 5,000 clones made
                     from the same 3 libraries. The pools consisted of
                     I.M.A.G.E. clones 297480-302087, 682632-687239,
                     726408-728711, and 729096-731399. Subtraction by Bento
                     Soares and M. Fatima Bonaldo."
ORIGIN      
        1 tttgttatgc ttgtatatga aaggatgtgt gttgtgggtt atgaggaaaa ttacattttt
       61 tacctgggat gaaattttaa aacttgaaag ctactgacca gaagcaacct gcacttgtgg
      121 acaaaagaaa tgcctaagaa tgttcccagc aaaacgcagt cctcagggcc ccaacctggc
      181 caaacactca tccacgggaa gatgaagaca tttcccatgc tcccctcaga caacgagaga
      241 ccatgcagca atgaaaatgg accatgtcag tgtgggtggg tctcagggag agaatggaga
      301 caggagatca cgcagcaatg aaaatgaacc atgtcagtgt gggtgggtct caaggagaga
      361 atggaggaca gaaatagaca tagagcagat gctcagagcc atgcagtgca ggagcagcca
      421 cgcaggagaa tttcctcaca tcaaagttca aaactacagc caaggcaaca gagcaagacc
      481 ctgcctcaaa aagaaaacaa agt
//
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