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GenBank: AI026002.1
FASTA Graphics
LOCUS AI026002 388 bp mRNA linear EST 06-JAN-2011 DEFINITION ow03c05.s1 Soares_parathyroid_tumor_NbHPA Homo sapiens cDNA clone IMAGE:1645736 3', mRNA sequence. ACCESSION AI026002 VERSION AI026002.1 DBLINK BioSample: SAMN00155028 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 388) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima Bonaldo, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 1069 Std Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 357. FEATURES Location/Qualifiers source 1..388 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:1645736" /tissue_type="parathyroid tumor" /clone_lib="SAMN00155028 Soares_parathyroid_tumor_NbHPA" /dev_stage="adult" /lab_host="DH10B (ampicillin resistant)" /note="Organ: parathyroid gland; Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5'- TGTTACCAATCTGAAGTGGGAGCGGCCGCACCAATTTTTTTTTTTTTTTTTTTTTTTT T-3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA from sporadic parathyroid adenomas was kindly provided by Dr. Stephen Marx, National Institute of Diabetes and Digestive and Kidney Diseases, NIH." ORIGIN 1 tttttgctaa aaatttacca aaatagtttg aacacataaa aatattttta aaaaaacaaa 61 accaaaaacc cagcataaat ttagttgtat aggcattggt tagaggacac tgttttcact 121 aaggattata ttcaacaact ttctcttgag ttgttactaa aattctgatt ctgaacctta 181 tagcttataa tggtgccaac tattagaaat gggaaaatct aattcagtcc aatgtaacat 241 gtattatgat ataatagatg aagggtatgt ctacactata ataaaaaata aacatatttt 301 tggttattta aagaccatct tcctaacctg taactaaaat aactgtattt gatttaaact 361 tatttaagtg cagtgaatta tggaaagc //
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