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ow03c05.s1 Soares_parathyroid_tumor_NbHPA Homo sapiens cDNA clone IMAGE:1645736 3', mRNA sequence

GenBank: AI026002.1

FASTA Graphics 

LOCUS       AI026002                 388 bp    mRNA    linear   EST 06-JAN-2011
DEFINITION  ow03c05.s1 Soares_parathyroid_tumor_NbHPA Homo sapiens cDNA clone
            IMAGE:1645736 3', mRNA sequence.
ACCESSION   AI026002
VERSION     AI026002.1
DBLINK      BioSample: SAMN00155028
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 388)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima
            Bonaldo, Ph.D.
             cDNA Library Arrayed by: Greg Lennon, Ph.D.
             DNA Sequencing by: Washington University Genome Sequencing Center
             Clone distribution: NCI-CGAP clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            www-bio.llnl.gov/bbrp/image/image.html
            
            Insert Length: 1069   Std Error: 0.00
            Seq primer: -40m13 fwd. ET from Amersham
            High quality sequence stop: 357.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:1645736"
                     /tissue_type="parathyroid tumor"
                     /clone_lib="SAMN00155028 Soares_parathyroid_tumor_NbHPA"
                     /dev_stage="adult"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: parathyroid gland; Vector: pT7T3D
                     (Pharmacia) with a modified polylinker; Site_1: Not I;
                     Site_2: Eco RI; 1st strand cDNA was primed with a Not I -
                     oligo(dT) primer
                     [5'-
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCACCAATTTTTTTTTTTTTTTTTTTTTTTT
                     T-3'], double-stranded cDNA was size selected, ligated to
                     Eco RI adapters (Pharmacia), digested with Not I and
                     cloned into the Not I and Eco RI sites of a modified pT7T3
                     vector (Pharmacia). Library went through one round of
                     normalization to a Cot = 5. Library constructed by Bento
                     Soares and M.Fatima Bonaldo. RNA from sporadic parathyroid
                     adenomas was kindly provided by Dr. Stephen Marx, National
                     Institute of Diabetes and Digestive and Kidney Diseases,
                     NIH."
ORIGIN      
        1 tttttgctaa aaatttacca aaatagtttg aacacataaa aatattttta aaaaaacaaa
       61 accaaaaacc cagcataaat ttagttgtat aggcattggt tagaggacac tgttttcact
      121 aaggattata ttcaacaact ttctcttgag ttgttactaa aattctgatt ctgaacctta
      181 tagcttataa tggtgccaac tattagaaat gggaaaatct aattcagtcc aatgtaacat
      241 gtattatgat ataatagatg aagggtatgt ctacactata ataaaaaata aacatatttt
      301 tggttattta aagaccatct tcctaacctg taactaaaat aactgtattt gatttaaact
      361 tatttaagtg cagtgaatta tggaaagc
//
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