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UI-R-C2-mz-c-06-0-UI.s1 UI-R-C2 Rattus norvegicus cDNA clone UI-R-C2-mz-c-06-0-UI 3', mRNA sequence

GenBank: AI071894.1

FASTA Graphics 

LOCUS       AI071894                 298 bp    mRNA    linear   EST 26-JAN-2011
DEFINITION  UI-R-C2-mz-c-06-0-UI.s1 UI-R-C2 Rattus norvegicus cDNA clone
            UI-R-C2-mz-c-06-0-UI 3', mRNA sequence.
ACCESSION   AI071894
VERSION     AI071894.1
DBLINK      BioSample: SAMN00155968
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            Oligo-dT track not found, Not I site shown in beginning of sequence
            is likely internal to the message. cDNA Library Preparation: M.
            Fatima Bonaldo, Ph.D. Clone distribution: clones will be available
            through Research Genetics This clone is also available through the
            I.M.A.G.E. Consortium at LLNL (info@image.llnl.gov). IMAGE
            ID=1786188
            Seq primer: M13 Forward
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-C2-mz-c-06-0-UI"
                     /clone_lib="SAMN00155968 UI-R-C2"
                     /dev_stage="adult"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-C2 library is a subtracted library derived from
                     the UI-R-C1 library, which is a subtracted library derived
                     from the UI-R-C0 library. The UI-R-C0 library consisted of
                     a mixture of individually tagged normalized libraries
                     constructed from rat placenta, adult lung, brain, liver,
                     kidney, heart, spleen, ovary, muscle, 8, 12 and 18-day
                     embryo. The tag is a string of 3-5 nucleotides present
                     between the Not I site and the oligo-dT track which allows
                     identification of the library of origin of a clone within
                     the mixture. The subtracted library (UI-R-C2) was
                     constructed as follows: PCR amplified cDNA inserts from
                     UI-R-C1 clones from which 3' ESTs had been derived was
                     used as a driver in a hybridization with the UI-R-C1
                     library in the form of single-stranded circles. The
                     remaining single-stranded circles (subtracted library) was
                     purified by hydroxyapatite column chromatography,
                     converted to double-stranded circles and electroporated
                     into DH10B bacteria (Life Technologies) to generate the
                     UI-R-C2 library. This procedure has been previously
                     described (Bonaldo, Lennon and Soares, Genome Research 6:
                     791-806, 1996)"
ORIGIN      
        1 cggccgcaaa ggttttttta agattaaaaa ttatgtcctc tcatgtggaa tctcatctgt
       61 cacaatggtt agacgatgca gaatagagca aggcttgttg tagtggtcca gcttacagta
      121 aaattcttga tgtttaaatg tgtttactta ctggctgtca tgtatatttt tgagattttt
      181 ctgcctgttc tgtgcagttt tctaaatgat actcctactt aaaaatagca ttttagtatc
      241 tattttctgt ctccattaaa tggtcctcat tttctattga gtttgcaagt gtgcacat
//
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