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GenBank: AI071894.1
FASTA Graphics
LOCUS AI071894 298 bp mRNA linear EST 26-JAN-2011 DEFINITION UI-R-C2-mz-c-06-0-UI.s1 UI-R-C2 Rattus norvegicus cDNA clone UI-R-C2-mz-c-06-0-UI 3', mRNA sequence. ACCESSION AI071894 VERSION AI071894.1 DBLINK BioSample: SAMN00155968 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 298) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org Oligo-dT track not found, Not I site shown in beginning of sequence is likely internal to the message. cDNA Library Preparation: M. Fatima Bonaldo, Ph.D. Clone distribution: clones will be available through Research Genetics This clone is also available through the I.M.A.G.E. Consortium at LLNL (info@image.llnl.gov). IMAGE ID=1786188 Seq primer: M13 Forward POLYA=No. FEATURES Location/Qualifiers source 1..298 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-C2-mz-c-06-0-UI" /clone_lib="SAMN00155968 UI-R-C2" /dev_stage="adult" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; The UI-R-C2 library is a subtracted library derived from the UI-R-C1 library, which is a subtracted library derived from the UI-R-C0 library. The UI-R-C0 library consisted of a mixture of individually tagged normalized libraries constructed from rat placenta, adult lung, brain, liver, kidney, heart, spleen, ovary, muscle, 8, 12 and 18-day embryo. The tag is a string of 3-5 nucleotides present between the Not I site and the oligo-dT track which allows identification of the library of origin of a clone within the mixture. The subtracted library (UI-R-C2) was constructed as follows: PCR amplified cDNA inserts from UI-R-C1 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the UI-R-C1 library in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (Life Technologies) to generate the UI-R-C2 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996)" ORIGIN 1 cggccgcaaa ggttttttta agattaaaaa ttatgtcctc tcatgtggaa tctcatctgt 61 cacaatggtt agacgatgca gaatagagca aggcttgttg tagtggtcca gcttacagta 121 aaattcttga tgtttaaatg tgtttactta ctggctgtca tgtatatttt tgagattttt 181 ctgcctgttc tgtgcagttt tctaaatgat actcctactt aaaaatagca ttttagtatc 241 tattttctgt ctccattaaa tggtcctcat tttctattga gtttgcaagt gtgcacat //
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