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ox75e08.x1 Soares_NhHMPu_S1 Homo sapiens cDNA clone IMAGE:1662182 3', mRNA sequence

GenBank: AI082183.1

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LOCUS       AI082183                 421 bp    mRNA    linear   EST 04-JAN-2011
DEFINITION  ox75e08.x1 Soares_NhHMPu_S1 Homo sapiens cDNA clone IMAGE:1662182
            3', mRNA sequence.
ACCESSION   AI082183
VERSION     AI082183.1
DBLINK      BioSample: SAMN00155192
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 421)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 533   Std Error: 0.00
            Seq primer: -40m13 fwd. ET from Amersham
            High quality sequence stop: 377.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:1662182"
                     /tissue_type="Pooled human melanocyte, fetal heart, and
                     pregnant uterus"
                     /clone_lib="SAMN00155192 Soares_NhHMPu_S1"
                     /lab_host="DH10B"
                     /note="Organ: mixed (see below); Vector: pT7T3D-PacI;
                     Site_1: Not I; Site_2: Eco RI; Equal amounts of plasmid
                     DNA from three normalized libraries (melanocyte 2NbHM,
                     pregnant uterus NbHPU, and fetal heart NbHH19W) were
                     mixed, and ss circles were made in vitro. Following HAP
                     purification, this DNA was used as tracer in a subtractive
                     hybridization reaction. The driver was PCR-amplified cDNAs
                     from pools of 5,000 clones made from the same 3 libraries.
                     The pools consisted of I.M.A.G.E. clones 260232-265223,
                     340488-345479, and 484488-489479."
ORIGIN      
        1 ttttttttta ttgcgatatc cactttatta tggtggcctg gaactgatcc tgtaatatct
       61 ccatggtatg cctgtgctgt acagggggta aagtgtgagg ggtcaagaat tttcatgcca
      121 gaaattccat agcagcctac tagaactctg gaagtatcgc ctttggctgc agtgccagat
      181 tctggtgagg atttcctgga ccccgattta tatctgaaaa aacactaaaa ataacagaag
      241 cagggcagga agtggctatg ataacaaagc tgggcttggt actttctctg gcttttgtgg
      301 ccaatattac tggactcccg tggtatcaag tttcataaaa gaaactcata caaagcagag
      361 atatcttaat tattttcaga ctggcatttc aaaaagtgtc aatttaagta agtattataa
      421 g
//
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