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ma54f12.y1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE:314543 5', mRNA sequence

GenBank: AI605664.1

FASTA Graphics 

LOCUS       AI605664                 463 bp    mRNA    linear   EST 15-MAR-2000
DEFINITION  ma54f12.y1 Soares mouse p3NMF19.5 Mus musculus cDNA clone
            IMAGE:314543 5', mRNA sequence.
ACCESSION   AI605664
VERSION     AI605664.1
DBLINK      BioSample: SAMN00155042
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Marra,M., Hillier,L., Kucaba,T., Martin,J., Beck,C., Wylie,T.,
            Underwood,K., Steptoe,M., Theising,B., Allen,M., Bowers,Y.,
            Person,B., Swaller,T., Gibbons,M., Pape,D., Harvey,N., Schurk,R.,
            Ritter,E., Kohn,S., Shin,T., Jackson,Y., Cardenas,M., McCann,R.,
            Waterston,R. and Wilson,R.
  TITLE     The WashU-NCI Mouse EST Project 1999
  JOURNAL   Unpublished
COMMENT     Contact: Marra M/WashU-NCI Mouse EST Project 1999
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: mouseest@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            This read is a RESEQUENCE of a previously sequenced mouse clone
            This read has been verified (found to hit its original self in the
            correct orientation)
            MGI:205159
            Seq primer: -40RP from Gibco
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:314543"
                     /clone_lib="SAMN00155042 Soares mouse p3NMF19.5"
                     /dev_stage="19.5 dpc total fetus"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Vector: pT7T3D (Pharmacia) with a modified
                     polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
                     was primed with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library went through one round of
                     normalization to a Cot = 5. Library constructed by Bento
                     Soares and M.Fatima Bonaldo. RNA was kindly provided by
                     Dr. Minoru Ko (Wayne State University)."
ORIGIN      
        1 tttccgcccc tgatggttag ttagccctgc tccaccaggg ctgatccccg agggaagacc
       61 tcacagcttt gtcgccatcc cttaaccccg gggcaggggt ggtgagatga caaaaccgag
      121 aaacctagtc catccgtcgg taagggaaga caaactgaat acatccccgt tatggaatac
      181 cccatgaaaa gaacaaggta gatgtacgga aacatcgagg ggaaactgtt gccacagact
      241 acaaattcct gcatttgtgc atctatgcat agtgcgaagg aatagctctg aaccaagaga
      301 aaacacccaa ggccaagacc agggttggag ggacaagact catacagaaa cgcccacagc
      361 tcgaacctgc cgctttgctc ttccgaagcc atcacgtgtt cacctgcaac cttactccta
      421 ggttatttgg cgggaatcgg cccctccccc tccttcataa cgg
//
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