LOCUS AI605664 463 bp mRNA linear EST 15-MAR-2000
DEFINITION ma54f12.y1 Soares mouse p3NMF19.5 Mus musculus cDNA clone
IMAGE:314543 5', mRNA sequence.
ACCESSION AI605664
VERSION AI605664.1
DBLINK BioSample: SAMN00155042
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 463)
AUTHORS Marra,M., Hillier,L., Kucaba,T., Martin,J., Beck,C., Wylie,T.,
Underwood,K., Steptoe,M., Theising,B., Allen,M., Bowers,Y.,
Person,B., Swaller,T., Gibbons,M., Pape,D., Harvey,N., Schurk,R.,
Ritter,E., Kohn,S., Shin,T., Jackson,Y., Cardenas,M., McCann,R.,
Waterston,R. and Wilson,R.
TITLE The WashU-NCI Mouse EST Project 1999
JOURNAL Unpublished
COMMENT Contact: Marra M/WashU-NCI Mouse EST Project 1999
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
Tel: 314 286 1800
Fax: 314 286 1810
Email: mouseest@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
This read is a RESEQUENCE of a previously sequenced mouse clone
This read has been verified (found to hit its original self in the
correct orientation)
MGI:205159
Seq primer: -40RP from Gibco
POLYA=No.
FEATURES Location/Qualifiers
source 1..463
/organism="Mus musculus"
/mol_type="mRNA"
/db_xref="taxon:10090"
/clone="IMAGE:314543"
/clone_lib="SAMN00155042 Soares mouse p3NMF19.5"
/dev_stage="19.5 dpc total fetus"
/lab_host="DH10B (ampicillin resistant)"
/note="Vector: pT7T3D (Pharmacia) with a modified
polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
was primed with a Not I - oligo(dT) primer [5'
TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was size selected, ligated to Eco RI
adapters (Pharmacia), digested with Not I and cloned into
the Not I and Eco RI sites of a modified pT7T3 vector
(Pharmacia). Library went through one round of
normalization to a Cot = 5. Library constructed by Bento
Soares and M.Fatima Bonaldo. RNA was kindly provided by
Dr. Minoru Ko (Wayne State University)."
ORIGIN
1 tttccgcccc tgatggttag ttagccctgc tccaccaggg ctgatccccg agggaagacc
61 tcacagcttt gtcgccatcc cttaaccccg gggcaggggt ggtgagatga caaaaccgag
121 aaacctagtc catccgtcgg taagggaaga caaactgaat acatccccgt tatggaatac
181 cccatgaaaa gaacaaggta gatgtacgga aacatcgagg ggaaactgtt gccacagact
241 acaaattcct gcatttgtgc atctatgcat agtgcgaagg aatagctctg aaccaagaga
301 aaacacccaa ggccaagacc agggttggag ggacaagact catacagaaa cgcccacagc
361 tcgaacctgc cgctttgctc ttccgaagcc atcacgtgtt cacctgcaac cttactccta
421 ggttatttgg cgggaatcgg cccctccccc tccttcataa cgg
//