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GenBank: AI832105.1
FASTA Graphics
LOCUS AI832105 507 bp mRNA linear EST 06-JAN-2011 DEFINITION td12g08.x1 NCI_CGAP_Co16 Homo sapiens cDNA clone IMAGE:2075486 3', mRNA sequence. ACCESSION AI832105 VERSION AI832105.1 DBLINK BioSample: SAMN00156117 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 507) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Ilan Kirsch, M.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Seq primer: -40UP from Gibco High quality sequence stop: 453. FEATURES Location/Qualifiers source 1..507 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:2075486" /tissue_type="colon tumor, RER+" /clone_lib="SAMN00156117 NCI_CGAP_Co16" /lab_host="DH10B" /note="Organ: colon; Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; Plasmid DNA from the normalized library NCI_CGAP_Co10 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1057416-1061255, and 1144584-1145351). Subtraction by Bento Soares and M. Fatima Bonaldo." ORIGIN 1 tcattgcagg gcctggtggt ggccgtcctc tactgtttcc tgaacagtga ggtaagttca 61 tggccgcctt ggaaccaagc acaggtactc acttgcttcc tgagatgctg cccagcctgg 121 tgccgcagcc cccacacatg cctgtcttcc gcgggctcga gttactgccc gggaccccac 181 agttctgtgt ccccatcaga gaacccacaa cgacacaggc aaacccactc ttctgggccc 241 agcttccaaa ccccgccgtc ctttcgtcca ccctaagtag catcgactgc cgtccgtctc 301 ggagccctct cgggaaagct cccggccggg tctagacaga atctgttttt ccccatggtc 361 ggcgtctcgc tcctgaccat agcttgtgag tcccagaaag ccgggcctgg gttggggggt 421 ggggctgcac agcgtcctgt gagtccctgg ggaggaagcc cggagccgag ctctgccctg 481 cgttcattcc cagccaggga ggctgtc //
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