U.S. flag

An official website of the United States government

bs30h02.y1 Drosophila melanogaster adult testis library Drosophila melanogaster cDNA clone bs30h02 5', mRNA sequence

GenBank: AI946804.2

FASTA Graphics 

LOCUS       AI946804                 616 bp    mRNA    linear   EST 13-MAY-2010
DEFINITION  bs30h02.y1 Drosophila melanogaster adult testis library Drosophila
            melanogaster cDNA clone bs30h02 5', mRNA sequence.
ACCESSION   AI946804
VERSION     AI946804.2
DBLINK      BioSample: SAMN00156857
KEYWORDS    EST.
SOURCE      Drosophila melanogaster (fruit fly)
  ORGANISM  Drosophila melanogaster
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Diptera; Brachycera;
            Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Andrews,J., Bouffard,G.G., Cheadle,C., Lu,J., Becker,K.G. and
            Oliver,B.
  TITLE     Gene discovery using computational and microarray analysis of
            transcription in the drosophila melanogaster testis
  JOURNAL   Genome Res. 10 (12), 2030-2043 (2000)
   PUBMED   11116097
COMMENT     On Aug 17, 1999 this sequence version replaced AI946804.1.
            Contact: Brian Oliver
            Laboratory of Cellular and Developmental Biology
            NIDDK, National Institutes of Health
            6 Center Drive MSC 2715, Bldg 6, Rm B1-13, Bethesda, MD 20892 USA
            Fax: (301) 496 5239
            Email: oliver@helix.nih.gov,
            http://www.niddk.nih.gov/intram/people/boliver.htm
            Tissue isolation and library construction performed at the National
            Institute of Diabetes and Digestive and Kidney Diseases, NIH (see
            http://www.niddk.nih.gov/intram/people/boliver.htm). DNA sequencing
            and analyses performed by National Institutes of Health Intramural
            Sequencing Center (NISC; see http://www.nisc.nih.gov).
            Plate: 30  row: h  column: 02
            Seq primer: M13RP1 reverse primer (ABI).
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Drosophila melanogaster"
                     /mol_type="mRNA"
                     /strain="y[*] w[67c1]/Y"
                     /db_xref="taxon:7227"
                     /clone="bs30h02"
                     /sex="male"
                     /clone_lib="SAMN00156857 Drosophila melanogaster adult
                     testis library"
                     /dev_stage="1-5 day adult"
                     /lab_host="SOLR (Stratagene)"
                     /note="Organ: testis; Vector: pBlueScript SK (Stratagene);
                     Site_1: EcoR I; Site_2: Xho I; Testes dissected from 1-5
                     day adult y[*] w[67c1]/Y males raised at 25oC. RNA
                     isolated using Trizol (Life Technologies) and a single
                     round of Poly(A)+ selection using Oligotex (Qiagen). cDNA
                     library constructed using Stratagene ZAP-cDNA syntesis
                     kit. Oligo dT-primed, size fractionated
                     1-6 kb, and directionally cloned at EcoRI and XhoI in
                     Uni-ZAP XR. Following a single round of amplification
                     pBlueScript SK phagemids were mass excised. A distribution
                     channel for clones is being sought, but not currently
                     available. Requests for clones cannot be honored."
ORIGIN      
        1 gcacgagcaa aactcataac ccccttctta ttattacctt tctgacgttg tacaagatac
       61 tcaatacagt acaccacata taatttcgaa atgcttttgg cccgtttaaa ccatgtgctg
      121 tcaggacgac cgatgcgcct tttgggccag tctggatgtc ctcgcctgtg gagctcagcc
      181 catgcgcaga tgaacagtaa ttccttaaga acaccttcct tggctcaagg aaagtcagtg
      241 attcctaaaa ggaatatgca agaacttggg atgagaaagt attccaggga gtcgagagat
      301 cgcgatcgtt cccaactgga gagtcgaacc agaggtcctt ccctcaagga gcgcatgatg
      361 ggacccccca gtgaaaatgc ttactccatg ggcaagggga gcgggcgcat ggagcggctc
      421 tgatgggtct cgtgggtctg tgctactatg gtctgggcct tgccaatcaa ccgagtatct
      481 acgaccactc gatggtgtgg ccccagtacg tgagggatcg tattcatgcc acctatgcct
      541 atttcggagc atcctgtggt gtaacagctg cttcagccgt tgcctttttc caatcggatg
      601 ccatgatggc tctgat
//
Feature
Display: FASTA GenBank Help
Details

Supplemental Content

Change region shown

Customize view

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
External link. Please review our privacy policy.