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GenBank: AI946804.2
FASTA Graphics
LOCUS AI946804 616 bp mRNA linear EST 13-MAY-2010 DEFINITION bs30h02.y1 Drosophila melanogaster adult testis library Drosophila melanogaster cDNA clone bs30h02 5', mRNA sequence. ACCESSION AI946804 VERSION AI946804.2 DBLINK BioSample: SAMN00156857 KEYWORDS EST. SOURCE Drosophila melanogaster (fruit fly) ORGANISM Drosophila melanogaster Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora. REFERENCE 1 (bases 1 to 616) AUTHORS Andrews,J., Bouffard,G.G., Cheadle,C., Lu,J., Becker,K.G. and Oliver,B. TITLE Gene discovery using computational and microarray analysis of transcription in the drosophila melanogaster testis JOURNAL Genome Res. 10 (12), 2030-2043 (2000) PUBMED 11116097 COMMENT On Aug 17, 1999 this sequence version replaced AI946804.1. Contact: Brian Oliver Laboratory of Cellular and Developmental Biology NIDDK, National Institutes of Health 6 Center Drive MSC 2715, Bldg 6, Rm B1-13, Bethesda, MD 20892 USA Fax: (301) 496 5239 Email: oliver@helix.nih.gov, http://www.niddk.nih.gov/intram/people/boliver.htm Tissue isolation and library construction performed at the National Institute of Diabetes and Digestive and Kidney Diseases, NIH (see http://www.niddk.nih.gov/intram/people/boliver.htm). DNA sequencing and analyses performed by National Institutes of Health Intramural Sequencing Center (NISC; see http://www.nisc.nih.gov). Plate: 30 row: h column: 02 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..616 /organism="Drosophila melanogaster" /mol_type="mRNA" /strain="y[*] w[67c1]/Y" /db_xref="taxon:7227" /clone="bs30h02" /sex="male" /clone_lib="SAMN00156857 Drosophila melanogaster adult testis library" /dev_stage="1-5 day adult" /lab_host="SOLR (Stratagene)" /note="Organ: testis; Vector: pBlueScript SK (Stratagene); Site_1: EcoR I; Site_2: Xho I; Testes dissected from 1-5 day adult y[*] w[67c1]/Y males raised at 25oC. RNA isolated using Trizol (Life Technologies) and a single round of Poly(A)+ selection using Oligotex (Qiagen). cDNA library constructed using Stratagene ZAP-cDNA syntesis kit. Oligo dT-primed, size fractionated 1-6 kb, and directionally cloned at EcoRI and XhoI in Uni-ZAP XR. Following a single round of amplification pBlueScript SK phagemids were mass excised. A distribution channel for clones is being sought, but not currently available. Requests for clones cannot be honored." ORIGIN 1 gcacgagcaa aactcataac ccccttctta ttattacctt tctgacgttg tacaagatac 61 tcaatacagt acaccacata taatttcgaa atgcttttgg cccgtttaaa ccatgtgctg 121 tcaggacgac cgatgcgcct tttgggccag tctggatgtc ctcgcctgtg gagctcagcc 181 catgcgcaga tgaacagtaa ttccttaaga acaccttcct tggctcaagg aaagtcagtg 241 attcctaaaa ggaatatgca agaacttggg atgagaaagt attccaggga gtcgagagat 301 cgcgatcgtt cccaactgga gagtcgaacc agaggtcctt ccctcaagga gcgcatgatg 361 ggacccccca gtgaaaatgc ttactccatg ggcaagggga gcgggcgcat ggagcggctc 421 tgatgggtct cgtgggtctg tgctactatg gtctgggcct tgccaatcaa ccgagtatct 481 acgaccactc gatggtgtgg ccccagtacg tgagggatcg tattcatgcc acctatgcct 541 atttcggagc atcctgtggt gtaacagctg cttcagccgt tgcctttttc caatcggatg 601 ccatgatggc tctgat //
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