Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
GenBank: AI946809.2
FASTA Graphics
LOCUS AI946809 666 bp mRNA linear EST 13-MAY-2010 DEFINITION bs30h07.y1 Drosophila melanogaster adult testis library Drosophila melanogaster cDNA clone bs30h07 5', mRNA sequence. ACCESSION AI946809 VERSION AI946809.2 DBLINK BioSample: SAMN00156857 KEYWORDS EST. SOURCE Drosophila melanogaster (fruit fly) ORGANISM Drosophila melanogaster Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora. REFERENCE 1 (bases 1 to 666) AUTHORS Andrews,J., Bouffard,G.G., Cheadle,C., Lu,J., Becker,K.G. and Oliver,B. TITLE Gene discovery using computational and microarray analysis of transcription in the drosophila melanogaster testis JOURNAL Genome Res. 10 (12), 2030-2043 (2000) PUBMED 11116097 COMMENT On Aug 17, 1999 this sequence version replaced AI946809.1. Contact: Brian Oliver Laboratory of Cellular and Developmental Biology NIDDK, National Institutes of Health 6 Center Drive MSC 2715, Bldg 6, Rm B1-13, Bethesda, MD 20892 USA Fax: (301) 496 5239 Email: oliver@helix.nih.gov, http://www.niddk.nih.gov/intram/people/boliver.htm Tissue isolation and library construction performed at the National Institute of Diabetes and Digestive and Kidney Diseases, NIH (see http://www.niddk.nih.gov/intram/people/boliver.htm). DNA sequencing and analyses performed by National Institutes of Health Intramural Sequencing Center (NISC; see http://www.nisc.nih.gov). Plate: 30 row: h column: 07 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..666 /organism="Drosophila melanogaster" /mol_type="mRNA" /strain="y[*] w[67c1]/Y" /db_xref="taxon:7227" /clone="bs30h07" /sex="male" /clone_lib="SAMN00156857 Drosophila melanogaster adult testis library" /dev_stage="1-5 day adult" /lab_host="SOLR (Stratagene)" /note="Organ: testis; Vector: pBlueScript SK (Stratagene); Site_1: EcoR I; Site_2: Xho I; Testes dissected from 1-5 day adult y[*] w[67c1]/Y males raised at 25oC. RNA isolated using Trizol (Life Technologies) and a single round of Poly(A)+ selection using Oligotex (Qiagen). cDNA library constructed using Stratagene ZAP-cDNA syntesis kit. Oligo dT-primed, size fractionated 1-6 kb, and directionally cloned at EcoRI and XhoI in Uni-ZAP XR. Following a single round of amplification pBlueScript SK phagemids were mass excised. A distribution channel for clones is being sought, but not currently available. Requests for clones cannot be honored." ORIGIN 1 gcaccagccg cactcttcgc tactttctct acgaaaatcg aaccgaaaaa tgggatccgg 61 caaacaacag aaagtccagt cctctggctt caccaaggaa gaggagctgc tgctgcagga 121 ctttagccgc aacgtgagca ccaagtcgtc ggcgctgttc tacggcaatg cctttatcgt 181 gtcggccgtt ccaatctggc tcttctggcg catccacaac atggacctgt ggcccagctc 241 catcctgttc gtcctggtga cggccgctag cacttatctg atggccaccg cctacaagaa 301 catcaagttc cagctgaagc acaagatcgc cggacggcgg gaggaggcgg tgacccgcga 361 ggtcaaccgt caggtgggcg atgacaagaa ggtcacgcgc aaggaaaagg acgagcgcat 421 cctgtggaag aagaacgagg tggccgacta cgaggccacc accttctcca tcttctacaa 481 caacgccatc tacctggccg ttatcatttt catcagcttc tttatcctga agaactcgac 541 gccgttcatc aactacatct tttccgtggg catcgccagc ggcgccctgg cgctcttctc 601 caccagcgca cagacgaact gagcgaatgt gcagccatgt catcccatgt tcatgtactc 661 gtaatg //
Whole sequence Selected region from: to:
All features Gene, RNA, and CDS features only
Show reverse complement Show gap features
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on