LOCUS AV215704 377 bp mRNA linear EST 30-OCT-1999
DEFINITION AV215704 RIKEN full-length enriched, ES cells Mus musculus cDNA
clone 2410151F13 3', mRNA sequence.
ACCESSION AV215704
VERSION AV215704.1
DBLINK BioSample: SAMN00157071
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 377)
AUTHORS Konno,H., Aizawa,K., Akahira,S., Akiyama,J., Carninci,P., Endo,T.,
Fukuda,S., Fukunishi,Y., Hara,A., Hayatsu,N., Hirozane,T., Hori,F.,
Ishii,Y., Ishikawa,T., Itoh,M., Izawa,M., Kadota,K., Kagawa,I.,
Kai,C., Kawai,J., Kikuchi,N., Kojima,Y., Koya,S., Kusakabe,M.,
Matsuyama,T., Miki,R., Mizuno,Y., Nakamura,M., Oda,H., Okazaki,Y.,
Owa,C., Ozawa,Y., Saito,H., Sano,M., Sato,K., Shibata,K.,
Shibata,Y., Shigemoto,Y., Shiraki,T., Sogabe,Y., Sugahara,Y.,
Suzuki,H., Suzuki,H., Takahashi,F., Tateno,M., Tominaga,N.,
Tsunoda,Y., Watahiki,A., Watanabe,S., Yamamura,T., Yasunishi,A.,
Yokota,T., Yoshiki,A., Yoshino,M., Muramatsu,M. and Hayashizaki,Y.
TITLE RIKEN Mouse ESTs (Konno,H., et al. 1999)
JOURNAL Unpublished (1999)
COMMENT Contact: Yoshihide Hayashizaki
Omics Science Center, RIKEN Yokohama Institute
The Institute of Physical and Chemical Research (RIKEN)
1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
Email: URL:http://www.osc.riken.jp/
Sasaki,N., Izawa,M., Watahiki,M., Ozawa,K., Tanaka,T., Yoneda,Y.,
Matsuura,S., Carninci,P., Muramatsu,M., Okazaki,Y. and
Hayashizaki,Y.
Transcriptional sequencing: A method for DNA sequencing using RNA
polymerase. Proc. Natl. Acad. Sci. U.S.A. 95 (7), 3455-3460 (1998)
Itoh,M., Kitsunai,T., Akiyama,J., Shibata,K., Izawa,M., Kawai,J.,
Tomaru,Y., Carninci,P., Shibata,Y., Ozawa,Y., Muramatsu,M.,
Okazaki,Y. and Hayashizaki,Y.
Automated filtration-based high-throughput plasmid preparation
system. Genome Res. 9 (5), 463-470 (1999)
Carninci,P. and Hayashizaki,Y.
High-efficiency full-length cDNA cloning. Methods Enzymol. 303,
19-44 (1999)
Please visit our web site (http://genome.rtc.riken.go.jp) for
further details.
FEATURES Location/Qualifiers
source 1..377
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="taxon:10090"
/clone="2410151F13"
/cell_type="ES cells"
/clone_lib="SAMN00157071 RIKEN full-length enriched, ES
cells"
/lab_host="SOLR"
/note="Site_1: XhoI; Site_2: BamHI; cDNA library was
prepared and sequenced in Mouse Genome Encyclopedia
Project of Genome Exploration Research Group in Riken
Genomic Sciences Center and Genome Science Laboratory in
RIKEN. Division of Experimental Animal Research in Riken
contributed to prepare mouse tissues. 1st strand cDNA was
primed with a primer [5'
GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was
prepared by using trehalose thermo-activated reverse
transcriptase and subsequently enriched for full-length by
cap-trapper. cDNA went through one round of normalization
to Rot = 5.0 and subtraction to Rot = 25.0. Second strand
cDNA was prepared with the primer adapter of sequence [5'
GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']."
ORIGIN
1 accctcccct tcccaccact gacccctctt attccctccc ttattccatc ctaccaatct
61 acttcctatc ttttcacacc ccccaagtgg ccatctaatt tcctcttccc atggttctgc
121 gccacctctt cttgctcgat ttgtattctg gacctctcca acagcactcc tgttcttatc
181 ccctgaccca tttctccaac ccttaagatt gtattttatt tccttttgat ttcctgcaag
241 tatcttggtt tcagttttct ttctggctgt aagactccat tgtatctccg aaccacattt
301 tatctttttt acgggccagt tggctgcttc tgttcttttg cctattgcat tcgctacagc
361 aataaacatg gatgtgt
//