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AV215704 RIKEN full-length enriched, ES cells Mus musculus cDNA clone 2410151F13 3', mRNA sequence

GenBank: AV215704.1

FASTA Graphics 

LOCUS       AV215704                 377 bp    mRNA    linear   EST 30-OCT-1999
DEFINITION  AV215704 RIKEN full-length enriched, ES cells Mus musculus cDNA
            clone 2410151F13 3', mRNA sequence.
ACCESSION   AV215704
VERSION     AV215704.1
DBLINK      BioSample: SAMN00157071
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Konno,H., Aizawa,K., Akahira,S., Akiyama,J., Carninci,P., Endo,T.,
            Fukuda,S., Fukunishi,Y., Hara,A., Hayatsu,N., Hirozane,T., Hori,F.,
            Ishii,Y., Ishikawa,T., Itoh,M., Izawa,M., Kadota,K., Kagawa,I.,
            Kai,C., Kawai,J., Kikuchi,N., Kojima,Y., Koya,S., Kusakabe,M.,
            Matsuyama,T., Miki,R., Mizuno,Y., Nakamura,M., Oda,H., Okazaki,Y.,
            Owa,C., Ozawa,Y., Saito,H., Sano,M., Sato,K., Shibata,K.,
            Shibata,Y., Shigemoto,Y., Shiraki,T., Sogabe,Y., Sugahara,Y.,
            Suzuki,H., Suzuki,H., Takahashi,F., Tateno,M., Tominaga,N.,
            Tsunoda,Y., Watahiki,A., Watanabe,S., Yamamura,T., Yasunishi,A.,
            Yokota,T., Yoshiki,A., Yoshino,M., Muramatsu,M. and Hayashizaki,Y.
  TITLE     RIKEN Mouse ESTs (Konno,H., et al. 1999)
  JOURNAL   Unpublished (1999)
COMMENT     Contact: Yoshihide Hayashizaki
            Omics Science Center, RIKEN Yokohama Institute
            The Institute of Physical and Chemical Research (RIKEN)
            1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
            Email: URL:http://www.osc.riken.jp/
            Sasaki,N., Izawa,M., Watahiki,M., Ozawa,K., Tanaka,T., Yoneda,Y.,
            Matsuura,S., Carninci,P., Muramatsu,M., Okazaki,Y. and
            Hayashizaki,Y.
             Transcriptional sequencing: A method for DNA sequencing using RNA
            polymerase. Proc. Natl. Acad. Sci. U.S.A. 95 (7), 3455-3460 (1998)
             Itoh,M., Kitsunai,T., Akiyama,J., Shibata,K., Izawa,M., Kawai,J.,
            Tomaru,Y., Carninci,P., Shibata,Y., Ozawa,Y., Muramatsu,M.,
            Okazaki,Y. and Hayashizaki,Y.
             Automated filtration-based high-throughput plasmid preparation
            system. Genome Res. 9 (5), 463-470 (1999)
             Carninci,P. and Hayashizaki,Y.
             High-efficiency full-length cDNA cloning. Methods Enzymol. 303,
            19-44 (1999)
             Please visit our web site (http://genome.rtc.riken.go.jp) for
            further details.
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /clone="2410151F13"
                     /cell_type="ES cells"
                     /clone_lib="SAMN00157071 RIKEN full-length enriched, ES
                     cells"
                     /lab_host="SOLR"
                     /note="Site_1: XhoI; Site_2: BamHI; cDNA library was
                     prepared and sequenced in Mouse Genome Encyclopedia
                     Project of Genome Exploration Research Group in Riken
                     Genomic Sciences Center and Genome Science Laboratory in
                     RIKEN. Division of Experimental Animal Research in Riken
                     contributed to prepare mouse tissues. 1st strand cDNA was
                     primed with a primer [5'
                     GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was
                     prepared by using trehalose thermo-activated reverse
                     transcriptase and subsequently enriched for full-length by
                     cap-trapper. cDNA went through one round of normalization
                     to Rot = 5.0 and subtraction to Rot = 25.0. Second strand
                     cDNA was prepared with the primer adapter of sequence [5'
                     GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']."
ORIGIN      
        1 accctcccct tcccaccact gacccctctt attccctccc ttattccatc ctaccaatct
       61 acttcctatc ttttcacacc ccccaagtgg ccatctaatt tcctcttccc atggttctgc
      121 gccacctctt cttgctcgat ttgtattctg gacctctcca acagcactcc tgttcttatc
      181 ccctgaccca tttctccaac ccttaagatt gtattttatt tccttttgat ttcctgcaag
      241 tatcttggtt tcagttttct ttctggctgt aagactccat tgtatctccg aaccacattt
      301 tatctttttt acgggccagt tggctgcttc tgttcttttg cctattgcat tcgctacagc
      361 aataaacatg gatgtgt
//
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