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214006 MARC 2BOV Bos taurus cDNA 5', mRNA sequence

GenBank: BE808737.1

FASTA Graphics 

LOCUS       BE808737                 536 bp    mRNA    linear   EST 08-MAY-2010
DEFINITION  214006 MARC 2BOV Bos taurus cDNA 5', mRNA sequence.
ACCESSION   BE808737
VERSION     BE808737.1
DBLINK      BioSample: SAMN00157377
KEYWORDS    EST.
SOURCE      Bos taurus (domestic cattle)
  ORGANISM  Bos taurus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Ruminantia;
            Pecora; Bovidae; Bovinae; Bos.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Smith,T.P.L., Grosse,W.M., Freking,B.A., Roberts,A.J., Stone,R.T.,
            Casas,E., Wray,J.E., White,J., Cho,J., Fahrenkrug,S.C.,
            Bennett,G.L., Heaton,M.P., Laegreid,W.W., Rohrer,G.A.,
            Chitko-McKown,C.G., Pertea,G., Holt,I., Karamycheva,S., Liang,F.,
            Quackenbush,J. and Keele,J.W.
  TITLE     Sequence evaluation of four pooled-tissue normalized bovine cDNA
            libraries and construction of a gene index for cattle
  JOURNAL   Genome Res. 11 (4), 626-630 (2001)
   PUBMED   11282978
COMMENT     Contact: Smith TPL
            USDA, ARS, US Meat Animal Research Center
            PO Box 166, Clay Center, NE 68933-0166, USA
            Tel: 402 762 4366
            Fax: 402 762 4390
            Email: Tim.Smith@ars.usda.gov
            Single pass sequencing. Bases called and alt_trimmed with phred
            v0.980904.e. Vector identified by cross_match with the -minscore 18
            and -minmatch 12 options.
            PCR PRimers
            FORWARD: AGGAAACAGCTATGACCAT
            BACKWARD: GTTTTCCCAGTCACGACG
            Plate: 69  row: L  column: 13
            Seq primer: ATTTAGGTGACACTATAG.
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Bos taurus"
                     /mol_type="mRNA"
                     /db_xref="taxon:9913"
                     /tissue_type="pooled"
                     /clone_lib="SAMN00157377 MARC 2BOV"
                     /lab_host="DH10B"
                     /note="Vector: pCMV SPORT6; Site_1: NotI; Site_2: SalI;
                     Library made from pooled tissue from testis, thymus,
                     semitendonosus muscle, longissimus muscle, pancreas,
                     adrenal, and endometrium."
ORIGIN      
        1 tactatattt attttgacaa agacatggac agggcgatgg agcactatgt ctctctgcct
       61 ggggacaagg ccgccgcgca catgcccctt ctcttcattt ccttctcgtc ggccaaggac
      121 ccaacttggg aggaccggta cccagaccgg tcgacagcga tcatcctagt gcccacctca
      181 tacgagtggt tcaaggagtg gcgggacgag ccccagggaa agcggagcag tgcctacgag
      241 aacctcaaga gctcctttgt tgaagccgct gtgtcggtct tcctgaagca gttcccacat
      301 ctggagggga aggtggacag tgtgactgga gggtccccac tgagcattca gttctacctg
      361 gcggctcccc agggggcctg ctacggggct gaccatgacc tgggccgcct gcatcctggt
      421 gcaatggcct ccataagggc ccagagcccc atcccgaacc tctacttgac aggccaagat
      481 gtcctcacct gtgggttgac tggagccctg caaggggcct tgctgtgcag cagtgc
//
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