Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
GenBank: BF404868.1
FASTA Graphics
LOCUS BF404868 507 bp mRNA linear EST 26-JAN-2011 DEFINITION UI-R-CA1-bic-f-04-0-UI.s1 UI-R-CA1 Rattus norvegicus cDNA clone UI-R-CA1-bic-f-04-0-UI 3', mRNA sequence. ACCESSION BF404868 VERSION BF404868.1 DBLINK BioSample: SAMN00161733 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 507) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org The sequence contained an oligo-dT track that was present in the oligonucleotide that was used to prime the synthesis of first strand cDNA and therefore this may represent a bonafide poly A tail. The sequence tag present in the cDNA between the NotI site and the oligo-dT track served to identify it as a clone from the normalized cerebellum library cDNA Library Preparation: M.B. Soares Lab Clone distribution: clones will be available through Research Genetics (www.resgen.com) Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..507 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-CA1-bic-f-04-0-UI" /clone_lib="SAMN00161733 UI-R-CA1" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; The UI-R-CA1 library is a subtracted library derived from the following tissues: thalamus, cerebellum, hypothalamus, medulla, pons, midbrain, cerebral cortex, corpus striatum, testis, and hippocampus. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996); TAG_LIB=UI-R-CA1; TAG_SEQ=CGGAAC; TAG_TISSUE=cerebellum" ORIGIN 1 tttttttttt tttttttaac ccagaaggca cagacctttt attacgttac atcttagaga 61 gcttaaacat gatattgtta cagctctcat cattctcttc taccagagcc agttggttgt 121 cttttactcc tatgtaagtg ccaggaagat tcttgcattc gaatgaaacg aagtcagagg 181 actttttgtg aagaacaaag aaggcctgat ctggcggaga gacatcaccc ttttggagct 241 ccacagagta gtccttgtca ttggcattca gccagatgtc tgtgtctttg ataggactca 301 tgttcaccat cagcttcttc ccatccacac cgtctcctga ttgtgcagga aaggaagact 361 cgtagtaacg gagtagcacc ttatcttttt cttggntttt cccacaatct tcaacattga 421 tcacgtaaca tccattctcc aaaacaaaac taacagattg gtcgttgtat gtgctcaggg 481 cacaagattc tgttagcagt gaagttc //
Whole sequence Selected region from: to:
All features Gene, RNA, and CDS features only
Show reverse complement Show gap features
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on