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UI-R-CA1-bic-f-04-0-UI.s1 UI-R-CA1 Rattus norvegicus cDNA clone UI-R-CA1-bic-f-04-0-UI 3', mRNA sequence

GenBank: BF404868.1

FASTA Graphics 

LOCUS       BF404868                 507 bp    mRNA    linear   EST 26-JAN-2011
DEFINITION  UI-R-CA1-bic-f-04-0-UI.s1 UI-R-CA1 Rattus norvegicus cDNA clone
            UI-R-CA1-bic-f-04-0-UI 3', mRNA sequence.
ACCESSION   BF404868
VERSION     BF404868.1
DBLINK      BioSample: SAMN00161733
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            The sequence contained an oligo-dT track that was present in the
            oligonucleotide that was used to prime the synthesis of first
            strand cDNA and therefore this may represent a bonafide poly A
            tail. The sequence tag present in the cDNA between the NotI site
            and the oligo-dT track served to identify it as a clone from the
            normalized cerebellum library cDNA Library Preparation: M.B. Soares
            Lab Clone distribution: clones will be available through Research
            Genetics (www.resgen.com)
            Seq primer: M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-CA1-bic-f-04-0-UI"
                     /clone_lib="SAMN00161733 UI-R-CA1"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-CA1 library is a subtracted library derived from
                     the following tissues: thalamus, cerebellum, hypothalamus,
                     medulla, pons, midbrain, cerebral cortex, corpus striatum,
                     testis, and hippocampus. For a detailed description of the
                     library from which this clone was derived, please visit
                     our web site at ratest.eng.uiowa.edu. The subtraction has
                     been previously described in (Bonaldo, Lennon and Soares,
                     Genome Research 6:791-806, 1996);
                     TAG_LIB=UI-R-CA1;
                     TAG_SEQ=CGGAAC;
                     TAG_TISSUE=cerebellum"
ORIGIN      
        1 tttttttttt tttttttaac ccagaaggca cagacctttt attacgttac atcttagaga
       61 gcttaaacat gatattgtta cagctctcat cattctcttc taccagagcc agttggttgt
      121 cttttactcc tatgtaagtg ccaggaagat tcttgcattc gaatgaaacg aagtcagagg
      181 actttttgtg aagaacaaag aaggcctgat ctggcggaga gacatcaccc ttttggagct
      241 ccacagagta gtccttgtca ttggcattca gccagatgtc tgtgtctttg ataggactca
      301 tgttcaccat cagcttcttc ccatccacac cgtctcctga ttgtgcagga aaggaagact
      361 cgtagtaacg gagtagcacc ttatcttttt cttggntttt cccacaatct tcaacattga
      421 tcacgtaaca tccattctcc aaaacaaaac taacagattg gtcgttgtat gtgctcaggg
      481 cacaagattc tgttagcagt gaagttc
//
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