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GenBank: BF551957.1
FASTA Graphics
LOCUS BF551957 428 bp mRNA linear EST 26-JAN-2011 DEFINITION UI-R-C2p-oc-f-11-0-UI.r1 UI-R-C2p Rattus norvegicus cDNA clone UI-R-C2p-oc-f-11-0-UI 5', mRNA sequence. ACCESSION BF551957 VERSION BF551957.1 DBLINK BioSample: SAMN00155992 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 428) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org cDNA Library Preparation: M.B. Soares Lab Clone distribution: clones will be available through Research Genetics (www.resgen.com) This clone is also available through the I.M.A.G.E. Consortium at LLNL (info@image.llnl.gov). IMAGE ID= 1787268 The following repetitive elements were found in this cDNA sequence: 341-361, >AT_rich#Low_complexity Seq primer: M13 Forward. FEATURES Location/Qualifiers source 1..428 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-C2p-oc-f-11-0-UI" /clone_lib="SAMN00155992 UI-R-C2p" /dev_stage="adult" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; The UI-R-C2p library is a subtracted library derived from the UI-R-C1 library, which is a subtracted library derived from the UI-R-C0 library. The UI-R-C0 library consisted of a mixture of individually tagged normalized libraries constructed from rat placenta, adult lung, brain, liver, kidney, heart, spleen, ovary, muscle, 8, 12 and 18-day embryo. The tag is a string of 3-5 nucleotides present between the Not I site and the oligo-dT track which allows identification of the library of origin of a clone within the mixture. The subtracted library (UI-R-C2p) was constructed as follows: PCR amplified cDNA inserts from UI-R-C1 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the UI-R-C1 library in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (Life Technologies) to generate the UI-R-C2p library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996)" ORIGIN 1 cacgaggaca gtttcttgtg cttggcctct cacacttgca gccttcctgc cctctcacct 61 tacctctgca tttaaatagt gcctttccct gtcagtgtga ggaccttctt agtgaatgtt 121 ggaggactgg cttgaagctt tttcctggga catcagcaca cggactgttc ttattagctg 181 gcaccccagg ctgctggagt tcctatcttg gcagtggacc atcaaacacc ctatttttaa 241 gagtctctga cttagttgta aacatgtgtt gtgactaggc tctctgtctc tttactcata 301 tttactgaag tggtttaact gttgaaaagt tgttatgatg aaaaaattaa aaaaaataaa 361 agcttggcct ccgggattgt tattttccac ttgtctaact caaaaataca tgatctcaga 421 aagaaagg //
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