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UI-R-C2p-oc-f-11-0-UI.r1 UI-R-C2p Rattus norvegicus cDNA clone UI-R-C2p-oc-f-11-0-UI 5', mRNA sequence

GenBank: BF551957.1

FASTA Graphics 

LOCUS       BF551957                 428 bp    mRNA    linear   EST 26-JAN-2011
DEFINITION  UI-R-C2p-oc-f-11-0-UI.r1 UI-R-C2p Rattus norvegicus cDNA clone
            UI-R-C2p-oc-f-11-0-UI 5', mRNA sequence.
ACCESSION   BF551957
VERSION     BF551957.1
DBLINK      BioSample: SAMN00155992
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            cDNA Library Preparation: M.B. Soares Lab Clone distribution:
            clones will be available through Research Genetics (www.resgen.com)
            This clone is also available through the I.M.A.G.E. Consortium at
            LLNL (info@image.llnl.gov). IMAGE ID= 1787268 The following
            repetitive elements were found in this cDNA sequence: 341-361,
            >AT_rich#Low_complexity
            Seq primer: M13 Forward.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-C2p-oc-f-11-0-UI"
                     /clone_lib="SAMN00155992 UI-R-C2p"
                     /dev_stage="adult"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-C2p library is a subtracted library derived from
                     the UI-R-C1 library, which is a subtracted library derived
                     from the UI-R-C0 library. The UI-R-C0 library consisted of
                     a mixture of individually tagged normalized libraries
                     constructed from rat placenta, adult lung, brain, liver,
                     kidney, heart, spleen, ovary, muscle, 8, 12 and 18-day
                     embryo. The tag is a string of 3-5 nucleotides present
                     between the Not I site and the oligo-dT track which allows
                     identification of the library of origin of a clone within
                     the mixture. The subtracted library (UI-R-C2p) was
                     constructed as follows: PCR amplified cDNA inserts from
                     UI-R-C1 clones from which 3' ESTs had been derived was
                     used as a driver in a hybridization with the UI-R-C1
                     library in the form of single-stranded circles. The
                     remaining single-stranded circles (subtracted library) was
                     purified by hydroxyapatite column chromatography,
                     converted to double-stranded circles and electroporated
                     into DH10B bacteria (Life Technologies) to generate the
                     UI-R-C2p library. This procedure has been previously
                     described (Bonaldo, Lennon and Soares, Genome Research 6:
                     791-806, 1996)"
ORIGIN      
        1 cacgaggaca gtttcttgtg cttggcctct cacacttgca gccttcctgc cctctcacct
       61 tacctctgca tttaaatagt gcctttccct gtcagtgtga ggaccttctt agtgaatgtt
      121 ggaggactgg cttgaagctt tttcctggga catcagcaca cggactgttc ttattagctg
      181 gcaccccagg ctgctggagt tcctatcttg gcagtggacc atcaaacacc ctatttttaa
      241 gagtctctga cttagttgta aacatgtgtt gtgactaggc tctctgtctc tttactcata
      301 tttactgaag tggtttaact gttgaaaagt tgttatgatg aaaaaattaa aaaaaataaa
      361 agcttggcct ccgggattgt tattttccac ttgtctaact caaaaataca tgatctcaga
      421 aagaaagg
//
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