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GenBank: BG375157.1
FASTA Graphics
LOCUS BG375157 417 bp mRNA linear EST 27-JAN-2011 DEFINITION UI-R-CV1-bsx-g-08-0-UI.s1 UI-R-CV1 Rattus norvegicus cDNA clone UI-R-CV1-bsx-g-08-0-UI 3', mRNA sequence. ACCESSION BG375157 VERSION BG375157.1 DBLINK BioSample: SAMN00163264 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 417) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org The sequence contained an oligo-dT track that was present in the oligonucleotide that was used to prime the synthesis of first strand cDNA and therefore this may represent a bonafide poly A tail. The sequence tag present in the cDNA between the NotI site and the oligo-dT track served to verify it as a clone from the normalized rat eye library cDNA Library Preparation: M.B. Soares Lab Clone distribution: clones will be available through Research Genetics (www.resgen.com) Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..417 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-CV1-bsx-g-08-0-UI" /clone_lib="SAMN00163264 UI-R-CV1" /dev_stage="ADULT" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; The UI-R-CV1 library is a normalized library constructed from rat eye tissue. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996); TAG_LIB=UI-R-CV1; TAG_SEQ=CAGCC; TAG_TISSUE=rat eye" ORIGIN 1 tttttttttt tttttgttgc tgttgttact tgtttttgtt ttgaatatca ggttccactg 61 cagtctagat tgtccttgaa ctcagtgctt agcccaagac gtccttgaat tcacagtgat 121 cttcctgctt agtcttccaa tcactgagac tagaaatgtg cacccaccag acttcattat 181 cagttgcttt aacaccggcc accatgtatg taccatgcct gcatataaaa gaagagggaa 241 ggccagcttc aattgccctt cactgtggta aagaccctgc agtcagcgct gtttgcccct 301 gaacacagat aagcacatca cagggaagca aagaaggttc ttggaggcgc agtggaactc 361 tggtttgcaa agcaaggagg ggagggaggg agggaacctc ttgaagctta ccggcta //
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