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UI-R-CV1-bsx-g-08-0-UI.s1 UI-R-CV1 Rattus norvegicus cDNA clone UI-R-CV1-bsx-g-08-0-UI 3', mRNA sequence

GenBank: BG375157.1

FASTA Graphics 

LOCUS       BG375157                 417 bp    mRNA    linear   EST 27-JAN-2011
DEFINITION  UI-R-CV1-bsx-g-08-0-UI.s1 UI-R-CV1 Rattus norvegicus cDNA clone
            UI-R-CV1-bsx-g-08-0-UI 3', mRNA sequence.
ACCESSION   BG375157
VERSION     BG375157.1
DBLINK      BioSample: SAMN00163264
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            The sequence contained an oligo-dT track that was present in the
            oligonucleotide that was used to prime the synthesis of first
            strand cDNA and therefore this may represent a bonafide poly A
            tail. The sequence tag present in the cDNA between the NotI site
            and the oligo-dT track served to verify it as a clone from the
            normalized rat eye library cDNA Library Preparation: M.B. Soares
            Lab Clone distribution: clones will be available through Research
            Genetics (www.resgen.com)
            Seq primer: M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-CV1-bsx-g-08-0-UI"
                     /clone_lib="SAMN00163264 UI-R-CV1"
                     /dev_stage="ADULT"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-CV1 library is a normalized library constructed
                     from rat eye tissue. For a detailed description of the
                     library from which this clone was derived, please visit
                     our web site at ratest.eng.uiowa.edu. The subtraction has
                     been previously described in (Bonaldo, Lennon and Soares,
                     Genome Research 6:791-806, 1996);
                     TAG_LIB=UI-R-CV1;
                     TAG_SEQ=CAGCC;
                     TAG_TISSUE=rat eye"
ORIGIN      
        1 tttttttttt tttttgttgc tgttgttact tgtttttgtt ttgaatatca ggttccactg
       61 cagtctagat tgtccttgaa ctcagtgctt agcccaagac gtccttgaat tcacagtgat
      121 cttcctgctt agtcttccaa tcactgagac tagaaatgtg cacccaccag acttcattat
      181 cagttgcttt aacaccggcc accatgtatg taccatgcct gcatataaaa gaagagggaa
      241 ggccagcttc aattgccctt cactgtggta aagaccctgc agtcagcgct gtttgcccct
      301 gaacacagat aagcacatca cagggaagca aagaaggttc ttggaggcgc agtggaactc
      361 tggtttgcaa agcaaggagg ggagggaggg agggaacctc ttgaagctta ccggcta
//
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