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AGENCOURT_6715286 NIH_MGC_100 Homo sapiens cDNA clone IMAGE:5798264 5', mRNA sequence

GenBank: BM928522.1

FASTA Graphics 

LOCUS       BM928522                1069 bp    mRNA    linear   EST 10-JAN-2011
DEFINITION  AGENCOURT_6715286 NIH_MGC_100 Homo sapiens cDNA clone IMAGE:5798264
            5', mRNA sequence.
ACCESSION   BM928522
VERSION     BM928522.1
DBLINK      BioSample: SAMN00164142
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1069)
  CONSRTM   National Institutes of Health, Mammalian Gene Collection (MGC)
  TITLE     NIH-MGC EST Sequencing Project
  JOURNAL   Unpublished
  REMARK    http://mgc.nci.nih.gov/
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: CGAP (Stanford)
             cDNA Library Preparation: Rubin Laboratory
             cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
             DNA Sequencing by: Agencourt Bioscience Corporation
             Clone distribution: MGC clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            http://image.llnl.gov
            
            Plate: LLCM2025  row: p  column: 09
            High quality sequence stop: 713.
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:5798264"
                     /tissue_type="hepatocellular carcinoma, cell line"
                     /clone_lib="SAMN00164142 NIH_MGC_100"
                     /lab_host="DH10B (phage-resistant)"
                     /note="Organ: liver; Vector: pOTB7; Site_1: XhoI; Site_2:
                     EcoRI; cDNA made by oligo-dT priming. Directionally cloned
                     into EcoRI/XhoI sites using the following 5' adaptor:
                     GGCACGAG(G). Size-selected >500bp for average insert size
                     1.8kb. Library constructed by Ling Hong in the laboratory
                     of Gerald M. Rubin (University of California, Berkeley)
                     using ZAP-cDNA synthesis kit (Stratagene) and Superscript
                     II RT (Life Technologies). Note: this is a NIH_MGC
                     Library."
ORIGIN      
        1 tggcgatttc ggcacgaggc agcagccttg gtgctcaggt gccagatgca aagaaagcat
       61 gtacctacat cagatctaac cttgatccca gcaatgtgga ttccctcttc tacgctgccc
      121 aggccagcca ggccctctca ggatgtgaga tctctatttc aaatgagacc aaagatctgc
      181 ttctggcagc tgtcagtgag gactcatctg ttacccagat ctaccatgca gttgcagctc
      241 taagtggctt tggccttccc ttggcatccc aagaagcact cagtgccctt actgctcgtc
      301 tcagcaagga ggagactgtg ctggcaacag tccaggctct gcagacagca tcccacctgt
      361 cccagcaggc tgacctgagg agcatcgtgg aggagattga ggaccttgtt gctcgcctgg
      421 atgaactcgg gggcgtgtat ctccagtttg aagaaggact ggaaacaaca gcgttatttg
      481 tggctgccac ctacaagctc atggatcatg tggggactga gccatccatt aaggaggatc
      541 aggtcatcca gctgatgaac gcgatcttca gcaagaagaa ctttgagtcc ctctccgaag
      601 ccttcagcgt ggcctctgca gctgctgtgc tctcgcataa tcgctaccac gtgccagttg
      661 tggttgtgcc tgagggctct gcttccgaca ctcatgaaca ggctatcttg cggttgcaag
      721 tcaccaatgt tctgtctcag cctctgactc aggccactgt taaactagaa catgctaaat
      781 ctgttgcttc cagagccact gtcctccaga agaatccttc acccctgtag gggatgtttt
      841 tgaactaaat ttcatgaacg tcaaattttt ccgtggttat tatgacttcc ttggtcaagt
      901 tgaaggtgga caaccggtat attgcaaata cccgtagagc tccaaagtca agaatctccc
      961 cctggaattg gccatcccac aaaggtgaat cctttcccac ccggggatta aggagttcca
     1021 agcctttggc cccccaaaaa cttaccccgg gggggacaat accccagcc
//
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