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GenBank: BM928522.1
FASTA Graphics
LOCUS BM928522 1069 bp mRNA linear EST 10-JAN-2011 DEFINITION AGENCOURT_6715286 NIH_MGC_100 Homo sapiens cDNA clone IMAGE:5798264 5', mRNA sequence. ACCESSION BM928522 VERSION BM928522.1 DBLINK BioSample: SAMN00164142 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1069) CONSRTM National Institutes of Health, Mammalian Gene Collection (MGC) TITLE NIH-MGC EST Sequencing Project JOURNAL Unpublished REMARK http://mgc.nci.nih.gov/ COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: CGAP (Stanford) cDNA Library Preparation: Rubin Laboratory cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Agencourt Bioscience Corporation Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLCM2025 row: p column: 09 High quality sequence stop: 713. FEATURES Location/Qualifiers source 1..1069 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:5798264" /tissue_type="hepatocellular carcinoma, cell line" /clone_lib="SAMN00164142 NIH_MGC_100" /lab_host="DH10B (phage-resistant)" /note="Organ: liver; Vector: pOTB7; Site_1: XhoI; Site_2: EcoRI; cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library." ORIGIN 1 tggcgatttc ggcacgaggc agcagccttg gtgctcaggt gccagatgca aagaaagcat 61 gtacctacat cagatctaac cttgatccca gcaatgtgga ttccctcttc tacgctgccc 121 aggccagcca ggccctctca ggatgtgaga tctctatttc aaatgagacc aaagatctgc 181 ttctggcagc tgtcagtgag gactcatctg ttacccagat ctaccatgca gttgcagctc 241 taagtggctt tggccttccc ttggcatccc aagaagcact cagtgccctt actgctcgtc 301 tcagcaagga ggagactgtg ctggcaacag tccaggctct gcagacagca tcccacctgt 361 cccagcaggc tgacctgagg agcatcgtgg aggagattga ggaccttgtt gctcgcctgg 421 atgaactcgg gggcgtgtat ctccagtttg aagaaggact ggaaacaaca gcgttatttg 481 tggctgccac ctacaagctc atggatcatg tggggactga gccatccatt aaggaggatc 541 aggtcatcca gctgatgaac gcgatcttca gcaagaagaa ctttgagtcc ctctccgaag 601 ccttcagcgt ggcctctgca gctgctgtgc tctcgcataa tcgctaccac gtgccagttg 661 tggttgtgcc tgagggctct gcttccgaca ctcatgaaca ggctatcttg cggttgcaag 721 tcaccaatgt tctgtctcag cctctgactc aggccactgt taaactagaa catgctaaat 781 ctgttgcttc cagagccact gtcctccaga agaatccttc acccctgtag gggatgtttt 841 tgaactaaat ttcatgaacg tcaaattttt ccgtggttat tatgacttcc ttggtcaagt 901 tgaaggtgga caaccggtat attgcaaata cccgtagagc tccaaagtca agaatctccc 961 cctggaattg gccatcccac aaaggtgaat cctttcccac ccggggatta aggagttcca 1021 agcctttggc cccccaaaaa cttaccccgg gggggacaat accccagcc //
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