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GenBank: BQ833497.1
FASTA Graphics
LOCUS BQ833497 646 bp mRNA linear EST 30-JAN-2011 DEFINITION LL6in21885 AFT024-subtracted library Mus musculus cDNA 5' similar to HSPC253, mRNA sequence. ACCESSION BQ833497 VERSION BQ833497.1 DBLINK BioSample: SAMN00153884 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 646) AUTHORS Hackney,J.A., Charbord,P., Brunk,B.P., Stoeckert,C.J., Lemischka,I.R. and Moore,K.A. TITLE A molecular profile of a hematopoietic stem cell niche JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (20), 13061-13066 (2002) PUBMED 12226475 COMMENT Contact: Moore, Kateri A. Department of Molecular Biology Princeton University 217 Lewis Thomas Laboratory, Washington Road, Princeton, NJ 08544, USA Tel: 609 258 0605 Fax: 609 258 2759 Email: kmoore@molbio.princeton.edu These ESTs are derived from a subtracted cDNA library enriched for gene products expressed by a hematopoietic stem cell-supporting stromal cell line, AFT024. Seq primer: M13Reverse or T7. FEATURES Location/Qualifiers source 1..646 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="taxon:10090" /cell_line="AFT024" /cell_type="Stromal cell" /tissue_type="Fetal Liver" /clone_lib="SAMN00153884 AFT024-subtracted library" /dev_stage="Embryonic day 14-14.5" /lab_host="DH10B" /note="Organ: Fetal Liver; Vector: Sport 1; Site_1: Sal I; Site_2: Not I; Two directionally cloned cDNA libraries were made from a hematopoietic stem cell-supporting stromal cell line (AFT024) and from a non-supporting stromal cell line (2018). Subtractive hybridization was performed by hybridization of the target, AFT024, single stranded cDNA library in pSport1 to biotinylated RNA transcribed from the driver, 2018 cDNA library in pSport2 with inserts cloned in the complementary orientation. The AFT024-subtracted library contains 4.2x105 clones and is depleted of common housekeeping gene products eg. beta-actin and enriched for transcripts specific to AFT024. For detailed protocols and additional information please see our website at http://stromalcell.princeton.edu." ORIGIN 1 cggaagtcgt gcaagttttc agcccggact acggaagaca tgcggaaggc tggttctagg 61 gctagggctg aggccgaagg tcctcaccgt gctatggagg gcggggaggt gactggcgac 121 cggctgaagg cagacacatc tgacatgtca tttgaggagc tgttgagatt acagggccaa 181 gggagaccca aggcacacaa acaattggta gctggaaaca gcactagaac gcgaagcccc 241 caacaacccg tgtgtgtcgc cgataagcac cggcctctgg aaatgtcagc taaagtccgg 301 gtgccattct tacgtcaggt ggtgcccatc agtaaaaagg tcgcccgaga cccccgcttt 361 gatgatctgt caggggacta taaccctgaa gtgtttgaca aaacttacca gttcctgaat 421 gatatccgag ccaaagagaa gcagcttgtg aaaaagcagt tgaagaagca ccgctcaggg 481 gaggagcatg acaaactgca acagctgctt cagcggatgg agcaacaaga gatggcacag 541 caggaacgca agcagcagca ggagctgcgc ttggccttga agcaggagcg ccgggctcag 601 gcacagcagg tccatcggac atacttcctg aagaaatctg agcagg //
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