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LL6in21885 AFT024-subtracted library Mus musculus cDNA 5' similar to HSPC253, mRNA sequence

GenBank: BQ833497.1

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LOCUS       BQ833497                 646 bp    mRNA    linear   EST 30-JAN-2011
DEFINITION  LL6in21885 AFT024-subtracted library Mus musculus cDNA 5' similar
            to HSPC253, mRNA sequence.
ACCESSION   BQ833497
VERSION     BQ833497.1
DBLINK      BioSample: SAMN00153884
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Hackney,J.A., Charbord,P., Brunk,B.P., Stoeckert,C.J.,
            Lemischka,I.R. and Moore,K.A.
  TITLE     A molecular profile of a hematopoietic stem cell niche
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 99 (20), 13061-13066 (2002)
   PUBMED   12226475
COMMENT     Contact: Moore, Kateri A.
            Department of Molecular Biology
            Princeton University
            217 Lewis Thomas Laboratory, Washington Road, Princeton, NJ 08544,
            USA
            Tel: 609 258 0605
            Fax: 609 258 2759
            Email: kmoore@molbio.princeton.edu
            These ESTs are derived from a subtracted cDNA library enriched for
            gene products expressed by a hematopoietic stem cell-supporting
            stromal cell line, AFT024.
            Seq primer: M13Reverse or T7.
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /cell_line="AFT024"
                     /cell_type="Stromal cell"
                     /tissue_type="Fetal Liver"
                     /clone_lib="SAMN00153884 AFT024-subtracted library"
                     /dev_stage="Embryonic day 14-14.5"
                     /lab_host="DH10B"
                     /note="Organ: Fetal Liver; Vector: Sport 1; Site_1: Sal I;
                     Site_2: Not I; Two directionally cloned cDNA libraries
                     were made from a hematopoietic stem cell-supporting
                     stromal cell line (AFT024) and from a non-supporting
                     stromal cell line (2018). Subtractive hybridization was
                     performed by hybridization of the target, AFT024, single
                     stranded cDNA library in pSport1 to biotinylated RNA
                     transcribed from the driver, 2018 cDNA library in pSport2
                     with inserts cloned in the complementary orientation. The
                     AFT024-subtracted library contains 4.2x105 clones and is
                     depleted of common housekeeping gene products eg.
                     beta-actin and enriched for transcripts specific to
                     AFT024. For detailed protocols and additional information
                     please see our website at
                     http://stromalcell.princeton.edu."
ORIGIN      
        1 cggaagtcgt gcaagttttc agcccggact acggaagaca tgcggaaggc tggttctagg
       61 gctagggctg aggccgaagg tcctcaccgt gctatggagg gcggggaggt gactggcgac
      121 cggctgaagg cagacacatc tgacatgtca tttgaggagc tgttgagatt acagggccaa
      181 gggagaccca aggcacacaa acaattggta gctggaaaca gcactagaac gcgaagcccc
      241 caacaacccg tgtgtgtcgc cgataagcac cggcctctgg aaatgtcagc taaagtccgg
      301 gtgccattct tacgtcaggt ggtgcccatc agtaaaaagg tcgcccgaga cccccgcttt
      361 gatgatctgt caggggacta taaccctgaa gtgtttgaca aaacttacca gttcctgaat
      421 gatatccgag ccaaagagaa gcagcttgtg aaaaagcagt tgaagaagca ccgctcaggg
      481 gaggagcatg acaaactgca acagctgctt cagcggatgg agcaacaaga gatggcacag
      541 caggaacgca agcagcagca ggagctgcgc ttggccttga agcaggagcg ccgggctcag
      601 gcacagcagg tccatcggac atacttcctg aagaaatctg agcagg
//
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