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UI-H-EI1-ayt-a-07-0-UI.s1 NCI_CGAP_EI1 Homo sapiens cDNA clone UI-H-EI1-ayt-a-07-0-UI 3', mRNA sequence

GenBank: CA449274.1

FASTA Graphics 

LOCUS       CA449274                 710 bp    mRNA    linear   EST 08-JAN-2011
DEFINITION  UI-H-EI1-ayt-a-07-0-UI.s1 NCI_CGAP_EI1 Homo sapiens cDNA clone
            UI-H-EI1-ayt-a-07-0-UI 3', mRNA sequence.
ACCESSION   CA449274
VERSION     CA449274.1
DBLINK      BioSample: SAMN00153784
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 710)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: Dr. Jose Mercuende
             cDNA Library preparation: Dr. M. Bento Soares, University of Iowa
             cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa
             DNA Sequencing by: Dr. M. Bento Soares, University of Iowa
             Clone Distribution: Clone distribution information can be obtained
            from Dr. M. Bento Soares, bento-soares@uiowa.edu
            
            Seq primer: M13 FORWARD
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="UI-H-EI1-ayt-a-07-0-UI"
                     /tissue_type="Chondrosarcoma"
                     /clone_lib="SAMN00153784 NCI_CGAP_EI1"
                     /dev_stage="Adult"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Organ: Left Pelvis; Vector: pT7T3-Pac (Pharmacia)
                     with a modified polylinker; Site_1: EcoR I; Site_2: Not I;
                     NCI_CGAP_EI1 is a normalized cDNA library containing the
                     following tissue(s): Chondrosarcoma. The library was
                     constructed according to Bonaldo, Lennon and Soares,
                     Genome Research, 6:791-806, 1996. First strand cDNA
                     synthesis was primed with an oligo-dT primer containing a
                     Not I site. Double stranded cDNA was ligated to an EcoR I
                     adaptor, digested with Not I, and cloned directionally
                     into pT7T3-Pac vector. The oligonucleotide used to prime
                     the synthesis of first-strand cDNA contains a library tag
                     sequence that is located between the Not I site and the
                     (dT)18 tail. The sequence tag for this library is
                     ACACTTGCAC.
                     TAG_LIB=UI-H-EI1;
                     TAG_SEQ=ACACTTGCAC;
                     TAG_TISSUE=chondrosarcoma"
ORIGIN      
        1 tttttttttt ttttttttag tgtttttacc atttttaata gtaacacaat aaatgagttt
       61 taataacatt aggaggcaat tcaagacata aaacagaacc tatttgtcct gtttgataag
      121 gcacaaggaa gagggcttcc tggaataaag ggattctcac agcagatgaa cacatttctg
      181 atttatctct ggtcagaggt gaatacaccg aaagataggc ctgagaggag gtgagaagga
      241 gcaattaggg atggtgtata taaggcagct ttggttaaca tcaacgaagc tcagttctag
      301 cttcacaatc caggaataat cctatgcggc tggtagttct tgggatatat tgcagtgtaa
      361 gtggggaggt ggtaaagaga ctgcactgaa tgtcattctt gacacatcca agactaaaga
      421 gtccctcctc tccatatata ttgtcattct gattcttccc tttccaatac ttattacaga
      481 caccaaaggc ccaattccaa gagtccccca catggacctc ccagtaatat ttgccagagg
      541 tgaaagtctg agcaccccat gcaagaaaac ttgtaggtgt tgcagtgata tggggcacat
      601 tttgacggtc acatccaata cacatgcttc tcaaatctcc acatcggaag atatgactgt
      661 tggcttcatt atgaggcaga gtaatatcca ctcagaattg gatgagcctg
//
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