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GenBank: CA449274.1
FASTA Graphics
LOCUS CA449274 710 bp mRNA linear EST 08-JAN-2011 DEFINITION UI-H-EI1-ayt-a-07-0-UI.s1 NCI_CGAP_EI1 Homo sapiens cDNA clone UI-H-EI1-ayt-a-07-0-UI 3', mRNA sequence. ACCESSION CA449274 VERSION CA449274.1 DBLINK BioSample: SAMN00153784 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 710) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Dr. Jose Mercuende cDNA Library preparation: Dr. M. Bento Soares, University of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa DNA Sequencing by: Dr. M. Bento Soares, University of Iowa Clone Distribution: Clone distribution information can be obtained from Dr. M. Bento Soares, bento-soares@uiowa.edu Seq primer: M13 FORWARD POLYA=Yes. FEATURES Location/Qualifiers source 1..710 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="UI-H-EI1-ayt-a-07-0-UI" /tissue_type="Chondrosarcoma" /clone_lib="SAMN00153784 NCI_CGAP_EI1" /dev_stage="Adult" /lab_host="DH10B (Life Technologies)" /note="Organ: Left Pelvis; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; NCI_CGAP_EI1 is a normalized cDNA library containing the following tissue(s): Chondrosarcoma. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is ACACTTGCAC. TAG_LIB=UI-H-EI1; TAG_SEQ=ACACTTGCAC; TAG_TISSUE=chondrosarcoma" ORIGIN 1 tttttttttt ttttttttag tgtttttacc atttttaata gtaacacaat aaatgagttt 61 taataacatt aggaggcaat tcaagacata aaacagaacc tatttgtcct gtttgataag 121 gcacaaggaa gagggcttcc tggaataaag ggattctcac agcagatgaa cacatttctg 181 atttatctct ggtcagaggt gaatacaccg aaagataggc ctgagaggag gtgagaagga 241 gcaattaggg atggtgtata taaggcagct ttggttaaca tcaacgaagc tcagttctag 301 cttcacaatc caggaataat cctatgcggc tggtagttct tgggatatat tgcagtgtaa 361 gtggggaggt ggtaaagaga ctgcactgaa tgtcattctt gacacatcca agactaaaga 421 gtccctcctc tccatatata ttgtcattct gattcttccc tttccaatac ttattacaga 481 caccaaaggc ccaattccaa gagtccccca catggacctc ccagtaatat ttgccagagg 541 tgaaagtctg agcaccccat gcaagaaaac ttgtaggtgt tgcagtgata tggggcacat 601 tttgacggtc acatccaata cacatgcttc tcaaatctcc acatcggaag atatgactgt 661 tggcttcatt atgaggcaga gtaatatcca ctcagaattg gatgagcctg //
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