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K0415F12-5N NIA Mouse Mesenchymal Stem Cell cDNA Library (Long) Mus musculus cDNA clone NIA:K0415F12 IMAGE:30060551 5', mRNA sequence

GenBank: CA567544.1

FASTA Graphics 

LOCUS       CA567544                 516 bp    mRNA    linear   EST 16-DEC-2010
DEFINITION  K0415F12-5N NIA Mouse Mesenchymal Stem Cell cDNA Library (Long) Mus
            musculus cDNA clone NIA:K0415F12 IMAGE:30060551 5', mRNA sequence.
ACCESSION   CA567544
VERSION     CA567544.1
DBLINK      BioSample: SAMN00169960
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Piao,Y., Kargul,G.J., Dudekula,D.B., Qian,Y., Luo,A., Carter,M.G.,
            Umezawa,A. and Ko,M.S.H.
  TITLE     Systematic Analyses of NIA Mouse Mesenchymal Stem Cell cDNA Library
            (Long)
  JOURNAL   Unpublished
COMMENT     Contact: Dawood B. Dudekula
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            Plate: K0415  row: F  column: 12
            Seq primer: M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C3H/He"
                     /db_xref="niaEST:K0415F12-5N"
                     /db_xref="taxon:10090"
                     /clone="NIA:K0415F12 IMAGE:30060551"
                     /cell_line="9-15-C cells"
                     /tissue_type="Mesenchymal stem cell"
                     /clone_lib="SAMN00169960 NIA Mouse Mesenchymal Stem Cell
                     cDNA Library (Long)"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1 (Invitrogen); Site_1: SalI; Site_2:
                     NotI; Mouse cDNA project by the Laboratory of Genetics,
                     National Institute on Aging (NIA), Intramural Research
                     Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is
                     a long-transcript enriched cDNA library (Ref. Genome Res.
                     11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were
                     obtained from Dr. Akihiro Umezawa (Keio University School
                     of Medicine, Japan). Double-stranded cDNAs were
                     synthesized with an Oligo(dT) primer [Invitrogen:
                     5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from
                     2.2 ug of total RNA, treated with T4 DNA polymerase, and
                     purified by ethanol-precipitation. The cDNAs were ligated
                     to Lone-linker LL-Sal4, purified by phenol/chloroform, and
                     separated from free linkers by Centricon 100. Then, the
                     cDNAs were amplified by long-range high fidelity PCR using
                     Ex Taq polymerase (Takara) with a primer Sal4-S. The
                     products were purified by phenol/chloroform and Centricon
                     100. The cDNAs were digested with SalI and NotI enzymes
                     and cloned into SalI/NotI site of pSPORT1 plasmid vector.
                     The DH10B E. coli host was transformed with the ligation
                     mixture by the standard chemical method. The average
                     insert size is about 2.5 kb. The library was constructed
                     by Yulan Piao (NIA)."
ORIGIN      
        1 taaaaaaaca aagcacactt ttctctggga acataattat tatcaccgac tatatccact
       61 ccagtagtga cccacgtgac aggcccaaat gcttggatgc agtcccaagg cataaagttc
      121 acagaaactt attctcctgg aaccatggca tcccgtataa tgaatgctcc aaacttgtcc
      181 ttgcatatgg atctgtctgc tttctttcag tattgtttta ttatagatgc ctccgagcaa
      241 tgacttgcca aatacccaag caaaattgaa ctttgcttcc tggccttcac cagtgtcctg
      301 ggtagttctt gagccaaccc tgggcttgga attcagtaag aatgttactt atttagtaac
      361 attcagaatt gcctctagta ttgtaagaga gatagtgaaa gaaatcaggc attactatct
      421 attacataga tttagaaatc tcagagcaag cgaagcaaaa tatacttaga atccccatta
      481 caatcaggta tggcagacta caatgcatat taagaa
//
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