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GenBank: CA567544.1
FASTA Graphics
LOCUS CA567544 516 bp mRNA linear EST 16-DEC-2010 DEFINITION K0415F12-5N NIA Mouse Mesenchymal Stem Cell cDNA Library (Long) Mus musculus cDNA clone NIA:K0415F12 IMAGE:30060551 5', mRNA sequence. ACCESSION CA567544 VERSION CA567544.1 DBLINK BioSample: SAMN00169960 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 516) AUTHORS Piao,Y., Kargul,G.J., Dudekula,D.B., Qian,Y., Luo,A., Carter,M.G., Umezawa,A. and Ko,M.S.H. TITLE Systematic Analyses of NIA Mouse Mesenchymal Stem Cell cDNA Library (Long) JOURNAL Unpublished COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: K0415 row: F column: 12 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..516 /organism="Mus musculus" /mol_type="mRNA" /strain="C3H/He" /db_xref="niaEST:K0415F12-5N" /db_xref="taxon:10090" /clone="NIA:K0415F12 IMAGE:30060551" /cell_line="9-15-C cells" /tissue_type="Mesenchymal stem cell" /clone_lib="SAMN00169960 NIA Mouse Mesenchymal Stem Cell cDNA Library (Long)" /lab_host="DH10B" /note="Vector: pSPORT1 (Invitrogen); Site_1: SalI; Site_2: NotI; Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Dr. Akihiro Umezawa (Keio University School of Medicine, Japan). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.2 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.5 kb. The library was constructed by Yulan Piao (NIA)." ORIGIN 1 taaaaaaaca aagcacactt ttctctggga acataattat tatcaccgac tatatccact 61 ccagtagtga cccacgtgac aggcccaaat gcttggatgc agtcccaagg cataaagttc 121 acagaaactt attctcctgg aaccatggca tcccgtataa tgaatgctcc aaacttgtcc 181 ttgcatatgg atctgtctgc tttctttcag tattgtttta ttatagatgc ctccgagcaa 241 tgacttgcca aatacccaag caaaattgaa ctttgcttcc tggccttcac cagtgtcctg 301 ggtagttctt gagccaaccc tgggcttgga attcagtaag aatgttactt atttagtaac 361 attcagaatt gcctctagta ttgtaagaga gatagtgaaa gaaatcaggc attactatct 421 attacataga tttagaaatc tcagagcaag cgaagcaaaa tatacttaga atccccatta 481 caatcaggta tggcagacta caatgcatat taagaa //
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