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IP05681.5prime IP Drosophila melanogaster iPCR Amplified Library Drosophila melanogaster cDNA clone IP05681 5, mRNA sequence

GenBank: EL875725.1

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LOCUS       EL875725                 527 bp    mRNA    linear   EST 06-MAR-2011
DEFINITION  IP05681.5prime IP Drosophila melanogaster iPCR Amplified Library
            Drosophila melanogaster cDNA clone IP05681 5, mRNA sequence.
ACCESSION   EL875725
VERSION     EL875725.1
DBLINK      BioSample: SAMN00152711
KEYWORDS    EST.
SOURCE      Drosophila melanogaster (fruit fly)
  ORGANISM  Drosophila melanogaster
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Diptera; Brachycera;
            Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Hoskins,R.A., Stapleton,M., George,R.A., Yu,C., Wan,K.H.,
            Carlson,J.W. and Celniker,S.E.
  TITLE     Rapid and efficient cDNA library screening by self-ligation of
            inverse PCR products (SLIP)
  JOURNAL   Nucleic Acids Res. 33 (21), e185 (2005)
   PUBMED   16326860
COMMENT     Contact: Carlson, J.
            BDGP
            Lawrence Berkeley National Lab
            One Cyclotron Rd, Berkeley, CA 94720, USA
            Fax: 510 486 7571
            Email: http://www.fruitfly.org/EST/index.shtml, cdna@fruitfly.org
            Based upon one or more reads of this clone where vector sequence
            was present at both ends, this clone has been determined to contain
            contain a cDNA insert on the order of 600-1000 bases.
            Plate: IP.56  row: G  column: 9
            High quality sequence stop: 526.
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Drosophila melanogaster"
                     /mol_type="mRNA"
                     /db_xref="taxon:7227"
                     /clone="IP05681"
                     /sex="male and female"
                     /clone_lib="SAMN00152711 IP Drosophila melanogaster iPCR
                     Amplified Library"
                     /dev_stage="mixed"
                     /lab_host="DH5-alpha"
                     /note="Organ: mixed; Vector: pOT2; Site_1: EcoRI; Site_2:
                     Xho1; iPCR amplified cDNA clones using gene specific
                     primers from a pool of GH (head), LD (embryo), LP (larva
                     and pupa), and SD (S2 cell line) libraries. Since clones
                     in this library have been enhanced for specific targets by
                     PCR, they cannot be used to quantify mRNA expression
                     levels."
ORIGIN      
        1 aatatgtctg acaaggataa gaaagataaa ccggaacagt tggatgatga ttccagtgac
       61 gacgatgcca accagagagc agttcgagat cagtattccg atcctaccat cagtgcatcc
      121 ccctcgattc tgcgacaccc caacgctccc cgtcctgata tttcaagtgc cccctcggtt
      181 gtcattgcgt tgccaagtag cgagaccctc gtcgtgaccg aggaaactcc ggaggagcga
      241 ctccggcgaa gaatgaggtt tctcgagttg cggcgtgagc actacaacta cgtcagccat
      301 agcgagcaat gcaacatggc ggatacggag gatgaagatc acacggagaa tcacccaaag
      361 tgtgaagcca attgcaagcc ggaatagaga ttggtttcat ttttttttaa tttttaatta
      421 aaactaccga aactaccttt caattaaatt agcataaagt aaaaaaaaaa aaaaaaaaaa
      481 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa
//
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