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yr87a03.s1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE:212236 3', mRNA sequence

GenBank: H69281.1

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LOCUS       H69281                   328 bp    mRNA    linear   EST 28-JAN-2011
DEFINITION  yr87a03.s1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone
            IMAGE:212236 3', mRNA sequence.
ACCESSION   H69281
VERSION     H69281.1
DBLINK      BioSample: SAMN00154876
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
            Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
            Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
            Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
            Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
            Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
            and Marra,M.
  TITLE     Generation and analysis of 280,000 human expressed sequence tags
  JOURNAL   Genome Res. 6 (9), 807-828 (1996)
   PUBMED   8889549
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            Insert Size: 1077
            High quality sequence stops: 274
            Source: IMAGE Consortium, LLNL
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 1077   Std Error: 0.00
            Seq primer: Promega -21m13
            High quality sequence stop: 274.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:3785077"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:212236"
                     /sex="male"
                     /clone_lib="SAMN00154876 Soares fetal liver spleen 1NFLS"
                     /dev_stage="20 week-post conception fetus"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
                     with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
                     1st strand cDNA was primed with a Pac I - oligo(dT) primer
                     [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Pac I and cloned into the Pac I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization. Library
                     constructed by Bento Soares and M.Fatima Bonaldo."
ORIGIN      
        1 nagnttcttg gaacaagtat ttatttaact ttttattatg gaatatttca aatacacata
       61 aaagtagaaa gantagcata ataaaccccc atgaacccat catccagctt caacaactaa
      121 catttttccc gatttaattt caactattca tcaccccccc atcttttgct tgagtatttt
      181 aaagcaaatt cagacatcat atcatttcac ttatgtatat ctnagtaaat gtctctacca
      241 gatgaggttt tgctttgtaa ttnaatcaca tgtcaaacct aacaaantta attctaatca
      301 tttaatatgg attnaacatc taggtctt
//
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