LOCUS H69281 328 bp mRNA linear EST 28-JAN-2011
DEFINITION yr87a03.s1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone
IMAGE:212236 3', mRNA sequence.
ACCESSION H69281
VERSION H69281.1
DBLINK BioSample: SAMN00154876
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 328)
AUTHORS Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
and Marra,M.
TITLE Generation and analysis of 280,000 human expressed sequence tags
JOURNAL Genome Res. 6 (9), 807-828 (1996)
PUBMED 8889549
COMMENT Contact: Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: est@watson.wustl.edu
Insert Size: 1077
High quality sequence stops: 274
Source: IMAGE Consortium, LLNL
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Insert Length: 1077 Std Error: 0.00
Seq primer: Promega -21m13
High quality sequence stop: 274.
FEATURES Location/Qualifiers
source 1..328
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="GDB:3785077"
/db_xref="taxon:9606"
/clone="IMAGE:212236"
/sex="male"
/clone_lib="SAMN00154876 Soares fetal liver spleen 1NFLS"
/dev_stage="20 week-post conception fetus"
/lab_host="DH10B (ampicillin resistant)"
/note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
1st strand cDNA was primed with a Pac I - oligo(dT) primer
[5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Pac I and cloned into the Pac I
and Eco RI sites of the modified pT7T3 vector. Library
went through one round of normalization. Library
constructed by Bento Soares and M.Fatima Bonaldo."
ORIGIN
1 nagnttcttg gaacaagtat ttatttaact ttttattatg gaatatttca aatacacata
61 aaagtagaaa gantagcata ataaaccccc atgaacccat catccagctt caacaactaa
121 catttttccc gatttaattt caactattca tcaccccccc atcttttgct tgagtatttt
181 aaagcaaatt cagacatcat atcatttcac ttatgtatat ctnagtaaat gtctctacca
241 gatgaggttt tgctttgtaa ttnaatcaca tgtcaaacct aacaaantta attctaatca
301 tttaatatgg attnaacatc taggtctt
//