LOCUS N66737 189 bp mRNA linear EST 28-JAN-2011
DEFINITION yz33g02.s1 Morton Fetal Cochlea Homo sapiens cDNA clone
IMAGE:284882 3', mRNA sequence.
ACCESSION N66737
VERSION N66737.1
DBLINK BioSample: SAMN00154983
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 189)
AUTHORS Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
and Marra,M.
TITLE Generation and analysis of 280,000 human expressed sequence tags
JOURNAL Genome Res. 6 (9), 807-828 (1996)
PUBMED 8889549
COMMENT Contact: Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: est@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Seq primer: m13 -40 forward.
FEATURES Location/Qualifiers
source 1..189
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="GDB:3891999"
/db_xref="taxon:9606"
/clone="IMAGE:284882"
/tissue_type="cochlea"
/clone_lib="SAMN00154983 Morton Fetal Cochlea"
/dev_stage="16-22 week fetus"
/lab_host="SOLR cells (kanamycin resistant)"
/note="Organ: ear; Vector: pBluescript SK-; Site_1: EcoRI;
Site_2: XhoI; Reference: Genomics 23, 42-50 (1994) Cloned
unidirectionally. Primer: Oligo dT. Fetal cochlea, normal.
37% of inserts <0.5 kb, 56% 0.5-1.0 kb, 7% >1 kb. Uni-ZAP
XR Vector. Library constructed by N. Robertson, C. Morton.
5' adaptor sequence: 5' GAATTCGGCACGAG 3'
3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'"
ORIGIN
1 tttccaataa tcttttcatt tttaatatca attgatgttt taaaaaatac agaggtgttt
61 gacacagaat agcaccattg tgtaggacac acacagttcc tgcgcccggc acctgaaggg
121 aggtcttctg gcctgggctg ggggcagtca ctcagggggc atttgactca caccagttag
181 tttcctgcc
//