LOCUS NM_001178428 2277 bp mRNA linear PLN 17-DEC-2024
DEFINITION Saccharomyces cerevisiae S288C AAA family ATPase SEC18 (SEC18),
partial mRNA.
ACCESSION NM_001178428
VERSION NM_001178428.3
DBLINK BioProject: PRJNA128
KEYWORDS RefSeq.
SOURCE Saccharomyces cerevisiae S288C
ORGANISM Saccharomyces cerevisiae S288C
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (bases 1 to 2277)
AUTHORS Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
Weng,S. and Cherry,J.M.
TITLE New data and collaborations at the Saccharomyces Genome Database:
updated reference genome, alleles, and the Alliance of Genome
Resources
JOURNAL Genetics 220 (4) (2022)
PUBMED 34897464
REFERENCE 2 (bases 1 to 2277)
AUTHORS Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
Oliver,S.G.
TITLE Life with 6000 genes
JOURNAL Science 274 (5287), 546 (1996)
PUBMED 8849441
REFERENCE 3 (bases 1 to 2277)
AUTHORS Feldmann,H., Aigle,M., Aljinovic,G., Andre,B., Baclet,M.C.,
Barthe,C., Baur,A., Becam,A.M., Biteau,N., Boles,E. et al.
TITLE Complete DNA sequence of yeast chromosome II
JOURNAL EMBO J. 13 (24), 5795-5809 (1994)
PUBMED 7813418
REFERENCE 4 (bases 1 to 2277)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (17-DEC-2024) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 5 (bases 1 to 2277)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (04-MAY-2012) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Protein update by submitter
REFERENCE 6 (bases 1 to 2277)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (31-MAR-2011) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Sequence update by submitter
REFERENCE 7 (bases 1 to 2277)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (11-DEC-2009) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
COMMENT REVIEWED REFSEQ: This record has been curated by SGD. This record
is derived from an annotated genomic sequence (NC_001134).
On Jul 30, 2012 this sequence version replaced NM_001178428.2.
##Genome-Annotation-Data-START##
Annotation Provider :: SGD
Annotation Status :: Full Annotation
Annotation Version :: R64-4-1
URL :: http://www.yeastgenome.org/
##Genome-Annotation-Data-END##
COMPLETENESS: incomplete on both ends.
FEATURES Location/Qualifiers
source 1..2277
/organism="Saccharomyces cerevisiae S288C"
/mol_type="mRNA"
/strain="S288C"
/db_xref="taxon:559292"
/chromosome="II"
gene <1..>2277
/gene="SEC18"
/locus_tag="YBR080C"
/gene_synonym="ANU4"
/db_xref="GeneID:852372"
CDS 1..2277
/gene="SEC18"
/locus_tag="YBR080C"
/gene_synonym="ANU4"
/experiment="EXISTENCE:direct assay:GO:0000149 SNARE
binding [PMID:27364524]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:23613772]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006888 endoplasmic
reticulum to Golgi vesicle-mediated transport
[PMID:9382859]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:10387016]"
/experiment="EXISTENCE:direct assay:GO:0035494 SNARE
complex disassembly [PMID:18650938|PMID:9425154]"
/experiment="EXISTENCE:direct assay:GO:0042144 vacuole
fusion, non-autophagic [PMID:9685264|PMID:8670830]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:direct assay:GO:0048280 vesicle
fusion with Golgi apparatus [PMID:9382859]"
/experiment="EXISTENCE:direct assay:GO:0070300
phosphatidic acid binding [PMID:27364524]"
/experiment="EXISTENCE:mutant phenotype:GO:0000045
autophagosome assembly [PMID:11694599]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:11694599]"
/experiment="EXISTENCE:mutant phenotype:GO:0043001 Golgi
to plasma membrane protein transport [PMID:2071670]"
/experiment="EXISTENCE:mutant phenotype:GO:0048219
inter-Golgi cisterna vesicle-mediated transport
[PMID:2071670|PMID:10637300]"
/note="AAA ATPase and SNARE disassembly chaperone;
required for vesicular transport between ER and Golgi, the
'priming' step in homotypic vacuole fusion, autophagy, and
protein secretion; releases Sec17p from SNAP complexes;
has similarity to mammalian N-ethylmaleimide-sensitive
factor (NSF)"
/codon_start=1
/product="AAA family ATPase SEC18"
/protein_id="NP_009636.3"
/db_xref="GeneID:852372"
/db_xref="SGD:S000000284"
/translation="MFKIPGFGKAAANHTPPDMTNMDTRTRHLKVSNCPNNSYALANV
AAVSPNDFPNNIYIIIDNLFVFTTRHSNDIPPGTIGFNGNQRTWGGWSLNQDVQAKAF
DLFKYSGKQSYLGSIDIDISFRARGKAVSTVFDQDELAKQFVRCYESQIFSPTQYLIM
EFQGHFFDLKIRNVQAIDLGDIEPTSAVATGIETKGILTKQTQINFFKGRDGLVNLKS
SNSLRPRSNAVIRPDFKFEDLGVGGLDKEFTKIFRRAFASRIFPPSVIEKLGISHVKG
LLLYGPPGTGKTLIARKIGTMLNAKEPKIVNGPEILSKYVGSSEENIRNLFKDAEAEY
RAKGEESSLHIIIFDELDSVFKQRGSRGDGTGVGDNVVNQLLAKMDGVDQLNNILVIG
MTNRKDLIDSALLRPGRFEVQVEIHLPDEKGRLQIFDIQTKKMRENNMMSDDVNLAEL
AALTKNFSGAEIEGLVKSASSFAINKTVNIGKGATKLNTKDIAKLKVTREDFLNALND
VTPAFGISEEDLKTCVEGGMMLYSERVNSILKNGARYVRQVRESDKSRLVSLLIHGPA
GSGKTALAAEIALKSGFPFIRLISPNELSGMSESAKIAYIDNTFRDAYKSPLNILVID
SLETLVDWVPIGPRFSNNILQMLKVALKRKPPQDRRLLIMTTTSAYSVLQQMDILSCF
DNEIAVPNMTNLDELNNVMIESNFLDDAGRVKVINELSRSCPNFNVGIKKTLTNIETA
RHDEDPVNELVELMTQSA"
ORIGIN
1 atgttcaaga tacctggttt tggaaaagct gctgcaaatc atactccacc agatatgaca
61 aacatggata cccgtacacg ccatttaaag gtgtcaaact gtccaaataa ctcctatgca
121 ctcgcaaacg tagctgctgt ctcaccaaat gatttcccta ataacattta tattattatc
181 gataatttat ttgttttcac aactagacac tccaacgaca ttccaccggg aaccattgga
241 tttaacggta accagcgtac ctggggtggt tggtccctaa atcaagacgt gcaagcaaaa
301 gcatttgatt tattcaagta ttccggtaag caatcgtatc ttggttcaat agatatagat
361 atctcattca gagctagagg taaggcggta agcacggtat tcgatcaaga tgagttagcc
421 aaacaatttg ttcgttgcta cgaatctcaa atattttctc ccacccagta ccttatcatg
481 gagttccaag gccatttctt tgacttaaaa attagaaatg tccaagcaat cgatttgggt
541 gatattgaac caacctccgc tgttgcaact gggatagaga caaagggaat tttgacaaaa
601 caaacacaaa ttaatttttt caaaggaaga gatggtttag ttaatttgaa atcatcaaat
661 tcattaagac caagatcaaa tgctgtgatc agaccggatt tcaagtttga agatttgggt
721 gtcggtggtt tggataaaga gtttactaaa attttcagaa gagcgtttgc aagtcgaatc
781 tttcctcctt cagttataga aaaactgggt atttctcatg ttaaaggttt gctattgtac
841 ggtcctccag gtactggtaa gaccttaatt gcaagaaaga ttggtacaat gctgaatgcc
901 aaagagccca aaatcgtcaa tggtccagaa attttgagta agtacgttgg ttcttcagaa
961 gaaaacattc gtaatttatt taaggatgca gaagcagaat atagggccaa gggtgaggaa
1021 tcttccttac atattattat tttcgatgag ctggattctg ttttcaagca gagaggttca
1081 agaggtgatg gtaccggtgt aggggacaat gtagttaatc aattgttagc taaaatggat
1141 ggtgttgatc aattgaataa tattttggtt attggtatga ccaatcgtaa agatttaata
1201 gacagtgctc ttttgcgtcc aggtagattt gaagtccaag ttgaaattca tttacccgat
1261 gaaaaaggaa gactccaaat tttcgacatt cagacgaaga aaatgaggga aaataatatg
1321 atgagcgacg atgttaactt agctgagtta gctgcgttaa caaaaaactt ctctggtgct
1381 gagattgagg gtttagtgaa gagtgcaagt tcttttgcaa tcaacaaaac cgtcaacatc
1441 gggaaaggtg ccacaaaact taacactaaa gatatagcaa aacttaaagt aacaagagaa
1501 gactttttaa atgcactcaa cgatgttact cccgcttttg ggattagtga agaagatttg
1561 aaaacatgtg tggaaggtgg aatgatgctt tattccgaac gagttaactc aatattgaag
1621 aacggagccc gttacgtccg ccaagttcgc gagagtgata aatccaggtt agtatctcta
1681 ttaatccacg gccctgcagg gtccggtaaa acagctttag ccgctgaaat tgctttaaaa
1741 tctggattcc cattcatcag gttaatttct cccaacgagt tgtcaggcat gtcagaaagc
1801 gcaaaaattg cctatattga taacactttc agagatgcgt ataaatctcc actaaacatt
1861 cttgttattg attcgttaga gactctagtt gattgggtac caattggtcc aagattctct
1921 aataacattt tacaaatgct aaaggttgca ttgaagcgta aacccccaca agaccgtcgt
1981 ttattgatca tgactactac atcagcttat tcggtacttc aacaaatgga tatcttgagt
2041 tgcttcgaca atgagatagc agttccaaat atgaccaatt tagatgaatt gaacaacgtc
2101 atgatagaat caaactttct tgacgatgct ggtagagtta aagttattaa tgaattatca
2161 aggagctgtc ctaacttcaa tgtcggtatt aaaaagacct tgaccaacat tgaaaccgca
2221 aggcacgatg aagatcccgt gaacgagctt gttgagttga tgacccaatc cgcataa
//