LOCUS NM_001270632 4086 bp mRNA linear ROD 08-DEC-2024
DEFINITION Rattus norvegicus brain-derived neurotrophic factor (Bdnf),
transcript variant 4, mRNA.
ACCESSION NM_001270632
VERSION NM_001270632.1
KEYWORDS RefSeq.
SOURCE Rattus norvegicus (Norway rat)
ORGANISM Rattus norvegicus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Rattus.
REFERENCE 1 (bases 1 to 4086)
AUTHORS Balanya-Segura,M., Polishchuk,A., Just-Borras,L., Cilleros-Mane,V.,
Silvera,C., Ardevol,A., Tomas,M., Lanuza,M.A., Hurtado,E. and
Tomas,J.
TITLE Molecular Adaptations of BDNF/NT-4 Neurotrophic and Muscarinic
Pathways in Ageing Neuromuscular Synapses
JOURNAL Int J Mol Sci 25 (15), 8018 (2024)
PUBMED 39125587
REMARK GeneRIF: Molecular Adaptations of BDNF/NT-4 Neurotrophic and
Muscarinic Pathways in Ageing Neuromuscular Synapses.
Publication Status: Online-Only
REFERENCE 2 (bases 1 to 4086)
AUTHORS Harada,H., Mori,M., Murata,Y., Kohno,Y., Terada,K., Ohe,K. and
Enjoji,M.
TITLE Divergent effects of chronic continuous and intermittent social
defeat stress on emotional behaviors: Impact on phosphorylated CREB
and BDNF protein levels in the rat hippocampus
JOURNAL Neurosci Lett 835, 137851 (2024)
PUBMED 38838971
REMARK GeneRIF: Divergent effects of chronic continuous and intermittent
social defeat stress on emotional behaviors: Impact on
phosphorylated CREB and BDNF protein levels in the rat hippocampus.
REFERENCE 3 (bases 1 to 4086)
AUTHORS Hamze,M., Brier,C., Buhler,E., Zhang,J., Medina,I. and Porcher,C.
TITLE Regulation of Neuronal Chloride Homeostasis by Pro- and Mature
Brain-Derived Neurotrophic Factor (BDNF) via KCC2 Cation-Chloride
Cotransporters in Rat Cortical Neurons
JOURNAL Int J Mol Sci 25 (11), 6253 (2024)
PUBMED 38892438
REMARK GeneRIF: Regulation of Neuronal Chloride Homeostasis by Pro- and
Mature Brain-Derived Neurotrophic Factor (BDNF) via KCC2
Cation-Chloride Cotransporters in Rat Cortical Neurons.
Publication Status: Online-Only
REFERENCE 4 (bases 1 to 4086)
AUTHORS Avarlaid,A., Falkenberg,K., Lehe,K., Mudo,G., Belluardo,N., Di
Liberto,V., Frinchi,M., Tuvikene,J. and Timmusk,T.
TITLE An upstream enhancer and MEF2 transcription factors fine-tune the
regulation of the Bdnf gene in cortical and hippocampal neurons
JOURNAL J Biol Chem 300 (6), 107411 (2024)
PUBMED 38796067
REMARK GeneRIF: An upstream enhancer and MEF2 transcription factors
fine-tune the regulation of the Bdnf gene in cortical and
hippocampal neurons.
REFERENCE 5 (bases 1 to 4086)
AUTHORS Campbell,T.S., Donoghue,K. and Roth,T.L.
TITLE Unlocking the epigenome: Stress and exercise induced Bdnf
regulation in the prefrontal cortex
JOURNAL Neurotoxicol Teratol 103, 107353 (2024)
PUBMED 38648864
REMARK GeneRIF: Unlocking the epigenome: Stress and exercise induced Bdnf
regulation in the prefrontal cortex.
REFERENCE 6 (bases 1 to 4086)
AUTHORS Ernfors,P., Van De Water,T., Loring,J. and Jaenisch,R.
TITLE Complementary roles of BDNF and NT-3 in vestibular and auditory
development
JOURNAL Neuron 14 (6), 1153-1164 (1995)
PUBMED 7605630
REMARK Erratum:[Neuron 1995 Sep;15(3):739]
REFERENCE 7 (bases 1 to 4086)
AUTHORS Ohara,O., Gahara,Y., Teraoka,H. and Kitamura,T.
TITLE A rat brain-derived neurotrophic factor-encoding gene generates
multiple transcripts through alternative use of 5' exons and
polyadenylation sites
JOURNAL Gene 121 (2), 383-386 (1992)
PUBMED 1446835
REFERENCE 8 (bases 1 to 4086)
AUTHORS Maisonpierre,P.C., Le Beau,M.M., Espinosa,R. 3rd, Ip,N.Y.,
Belluscio,L., de la Monte,S.M., Squinto,S., Furth,M.E. and
Yancopoulos,G.D.
TITLE Human and rat brain-derived neurotrophic factor and neurotrophin-3:
gene structures, distributions, and chromosomal localizations
JOURNAL Genomics 10 (3), 558-568 (1991)
PUBMED 1889806
REFERENCE 9 (bases 1 to 4086)
AUTHORS Hallbook,F., Ibanez,C.F. and Persson,H.
TITLE Evolutionary studies of the nerve growth factor family reveal a
novel member abundantly expressed in Xenopus ovary
JOURNAL Neuron 6 (5), 845-858 (1991)
PUBMED 2025430
REFERENCE 10 (bases 1 to 4086)
AUTHORS Yancopoulos,G.D., Maisonpierre,P.C., Ip,N.Y., Aldrich,T.H.,
Belluscio,L., Boulton,T.G., Cobb,M.H., Squinto,S.P. and Furth,M.E.
TITLE Neurotrophic factors, their receptors, and the signal transduction
pathways they activate
JOURNAL Cold Spring Harb Symp Quant Biol 55, 371-379 (1990)
PUBMED 1966766
COMMENT VALIDATED REFSEQ: This record has undergone validation or
preliminary review. The reference sequence was derived from
EF125677.1 and CN544668.1.
Transcript Variant: This variant (4, also known as IIB) has an
alternate 5' exon and uses a downstream start codon, compared to
variant 1. The resulting isoform (3) has a shorter N-terminus,
compared to isoform 1. Variants 3-10 encode the same isoform 3.
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: EF125677.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns
SAMN12840113, SAMN12840116
[ECO:0000348]
##Evidence-Data-END##
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-162 EF125677.1 1-162
163-4029 EF125677.1 164-4030
4030-4086 CN544668.1 1-57 c
FEATURES Location/Qualifiers
source 1..4086
/organism="Rattus norvegicus"
/mol_type="mRNA"
/strain="Sprague-Dawley"
/db_xref="taxon:10116"
/chromosome="3"
/map="3q34"
gene 1..4086
/gene="Bdnf"
/note="brain-derived neurotrophic factor"
/db_xref="GeneID:24225"
/db_xref="RGD:2202"
exon 1..416
/gene="Bdnf"
/inference="alignment:Splign:2.1.0"
misc_feature 396..398
/gene="Bdnf"
/note="upstream in-frame stop codon"
exon 417..4074
/gene="Bdnf"
/inference="alignment:Splign:2.1.0"
CDS 438..1187
/gene="Bdnf"
/note="isoform 3 preproprotein is encoded by transcript
variant 4; brain derived neurothrophic factor; proBDNF;
neurotrophic factor BDNF precursor form"
/codon_start=1
/product="neurotrophic factor BDNF precursor form isoform
3 preproprotein"
/protein_id="NP_001257561.1"
/db_xref="GeneID:24225"
/db_xref="RGD:2202"
/translation="MTILFLTMVISYFGCMKAAPMKEANVHGQGNLAYPAVRTHGTLE
SVNGPRAGSRGLTTTSLADTFEHVIEELLDEDQKVRPNEENHKDADLYTSRVMLSSQV
PLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAADKKTAVD
MSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVR
ALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR"
sig_peptide 438..491
/gene="Bdnf"
/inference="COORDINATES: ab initio prediction:SignalP:6.0"
mat_peptide 492..1184
/gene="Bdnf"
/product="Neurotrophic factor BDNF precursor form.
/id=PRO_0000447540"
/note="propagated from UniProtKB/Swiss-Prot (P23363.1)"
misc_feature 612..617
/gene="Bdnf"
/note="Cleavage, by MBTPS1.
/evidence=ECO:0000250|UniProtKB:P23560; propagated from
UniProtKB/Swiss-Prot (P23363.1); cleavage site"
misc_feature 804..806
/gene="Bdnf"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000255; propagated from
UniProtKB/Swiss-Prot (P23363.1); glycosylation site"
ORIGIN
1 gctttggcaa agccatccgc acgtgacaaa acgtaaggaa gtggaagaaa ccgtctagag
61 caatatcaag taccacttaa ttagagaata tttttttaac cttttcctcc tgctgcgccg
121 ggtgtgtgat ccgggcgagc agagtccatt cagcaccttg gacagagcca gcggatttgt
181 ccgaggtggt agtacttcat ccaggtattc ttttcctcgc tgtcaagcca acccggtgtc
241 gcccttaaaa agcgtctttt ccgaggttcg gctcacactg agatcggggc tggagagaga
301 gtcagatttt ggagcggagc gtttggagag ccagccccag tttggtcccc tcattgagct
361 cgctgaagtt ggcttcctag cggtgtaggc tggaatagac tcttggcaag ctccggttcc
421 accaggtgag aagagtgatg accatccttt tccttactat ggttatttca tacttcggtt
481 gcatgaaggc tgcgcccatg aaagaagcaa acgtccacgg acaaggcaac ttggcctacc
541 cagctgtgcg gacccatggg actctggaga gcgtgaatgg gcccagggca ggttcgagag
601 gtctgacgac gacgtccctg gctgacactt ttgagcacgt gatcgaagag ctgctggatg
661 aggaccagaa ggttcggccc aacgaagaaa accataagga cgcggacttg tacacttccc
721 gggtgatgct cagcagtcaa gtgcctttgg agcctcctct gctctttctg ctggaggaat
781 acaaaaatta cctggatgcc gcaaacatgt ctatgagggt tcggcgccac tccgaccccg
841 cccgccgtgg ggagctgagc gtgtgtgaca gtattagcga gtgggtcaca gcggcagata
901 aaaagactgc agtggacatg tccggtggga cggtcacagt cctggagaaa gtcccggtat
961 caaaaggcca actgaagcaa tatttctacg agaccaagtg taatcccatg ggttacacga
1021 aggaaggctg caggggcata gacaaaaggc actggaactc gcaatgccga actacccaat
1081 cgtatgttcg ggcccttact atggatagca aaaagagaat tggctggcgg ttcataagga
1141 tagacacttc ctgtgtatgt acactgacca ttaaaagggg aagatagtgg atttatgttg
1201 tatagattat attgagacaa aaattatcta tttgtatata tacataacag ggtaaattat
1261 tcagttaaga aaaagataat tttatgaact gcatgtataa atgaagttta tacagtacag
1321 tggttctaca atctatttat tggacatatc catgaccaga aagaaacagt catttgcgca
1381 caactttaaa agtctgcatt acattcctcg ataatgttgt ggtttgttgc cgttgccaag
1441 aattgaaaac aaaaagttaa aaaaaataat aaattgcatg ctgctttaat tgtgaattga
1501 taataaactg tccctctttc agaaaacaga caaaaaaaca aaaaacaaaa aaaagcaaaa
1561 acaaaaattt gaaccaaaac attccgttta cattttagac actaagtatc ttcgttcttg
1621 ttagtactct gttctactgc tttcaacttc tcatagcgtt ggaattaaaa ctatgtcaag
1681 gtgctgttgt cattgcttta ctggcttagg ggatggggaa cgggaggggt agatttctgt
1741 ttgttttgtg ttttatttcg tttgtttgtt tgttttgttt ttttagttcc acccggagta
1801 gggatggaga aaatttcttc actctccatt ctggttgata aagcgttaca tttgtatgtt
1861 gtaaaaaatg tttgcaaaat ccaatcagat gactggaaaa cgaataaaaa ttaaggcaac
1921 tgaataaaat gctcacacaa cactgcccat gatgtatctc cctggtcccc caggtcactc
1981 ttctggcatg ggtcagggaa agctgctttt attggaaaga ccagcatttg tttaaagcac
2041 attctttccc tccctcctcc cattttggtc cctttctttt ttgttttgtt ttaagaaaga
2101 aaattaagtt gcgcgctttg aaatatttta tcactgctgt gaacagatga acaatgtgtg
2161 tcatttcatg acactcgtgg aaaacagtga tttttttttt attttttgcc ctaaggagaa
2221 acaagtaaga ataaccgaaa atgttctttt tttttaaagg cataaacagt ggataagtta
2281 taatatggcc taacaatgtt tgcagataaa agatattgca tacagccaga tactagagca
2341 gggatccaca ctgccactga aatgcgactg aatggccctg tggaggctaa gtggagctga
2401 catactattt cctggcagag caggaggaat ttctgagtgg ccatcctgag gtctagatgg
2461 aggtggggaa tggtacttga gacattccta aaggaaggct cggaagcacc cttcagagca
2521 ggctctggaa tgatgtgtca agtttcttag gccttctgct ttaagtgcct acgttaccta
2581 acagtgctca agaggttctc aattggagaa ccacactcaa atccatttat ggcctccatc
2641 ccattttaaa taattatgga taaagttgga ttaacctgga gcagctttgg atccaaatat
2701 ggcatagcag tgatgctatc agtgcagcat gatgggaaat gtttgctgtg aagagactta
2761 actttctttg cgcttagact tcaggaagcc taggttttat ttatttattt ttttgagaca
2821 ttttggtgaa aggaaaaaag aaagaagaaa acaaacaaac aaaaccagaa aaagcaccaa
2881 aacttaggca gaatgagcaa tgtctgtctg taagggctag aatgacaagg cataggaagg
2941 tgctttcact gtgaaagaga caagaacaca ggaggaaata ctgcttaagt gaagagcaca
3001 gaaagctcct gatagttctg tccattcagc acaagggtcc cttctacact ttacctcttg
3061 gggttaggag aagtcaagct ggaagcctga atgaatggcc ccaatgagaa ctagtgttaa
3121 gcccatttcc ctagtgaggt tttccgccag cgcgaatgtg ttagtggtta cctgactggg
3181 ctcctgggca tcagaaaaag aggcaaacaa ttgcttcatc ttaggagtgg aaagggtgaa
3241 acaaagtggc tgtccactgt gactcaggga gtgaagatac catcagcaaa tagtttcttt
3301 ttgttcaact gttcctttag aactagtctg tcttctggag tcccactgaa tcccctgttt
3361 ttggaagact tcacgtagcc tagattgttt tgtgccgttt gacaacatta atctctgtca
3421 tcatttttaa cctattaagg aatgctttga atatctgcta tatgctaact ttttgcagct
3481 tcattctgag agacgttagt caaacaaata aaaggagccc catcacaatc tcacggtatt
3541 cgaagggcca ggtcgattag gtggcttcat aggagaccct ccgcaactgt gtggtcagtg
3601 gctggctctc atacccacta agatacatca tagctccatg tcggtggttt atgttgacct
3661 gagattgatt tgttaaaatc tctcctctgt ttctgttcgt tctgtttccg tcctgttctg
3721 ttctgttctg ttctgaaagt cttgctgtgg tctctttttg gcagaagtgt ttcatgcatg
3781 gcagcaggcc tgatgctttt tatagtgatt cccattgaaa ctgtaagtaa atgtctgtgg
3841 ccttgttctc tctatggtaa agatattatt caccatgtaa aacaagaaaa atatttattg
3901 tattttagta tatttatata attatgttat tgaaaaaaat tggcattaaa acttaaccac
3961 atcagaagcc tattgtaaat acaggttcta tttaagtgta ccaattaaca tataatatat
4021 gttttaaata tagaattttt aatgttttta aatatatttt caaagtacat aaaaaaaaaa
4081 aaaaaa
//