LOCUS NM_001376520 5083 bp mRNA linear PRI 29-OCT-2024
DEFINITION Homo sapiens mediator complex subunit 23 (MED23), transcript
variant 8, mRNA.
ACCESSION NM_001376520
VERSION NM_001376520.1
KEYWORDS RefSeq.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5083)
AUTHORS Li,Y., Sun,Y., Jia,B., Ma,Z. and Zhou,R.
TITLE MED23 depletion induces premature senescence in NSCLC cells by
interacting with BCLAF1 and then suppressing NUPR1 expression
JOURNAL Biochem Biophys Res Commun 734, 150754 (2024)
PUBMED 39366174
REMARK GeneRIF: MED23 depletion induces premature senescence in NSCLC
cells by interacting with BCLAF1 and then suppressing NUPR1
expression.
REFERENCE 2 (bases 1 to 5083)
AUTHORS Bamaga,A., Muthaffar,O., Alyazidi,A., Bahowarth,S., Shawli,M.,
Alotibi,F., Alsehemi,M., Almohammal,M., Alawwadh,A. and Alghamdi,N.
TITLE MED23 pathogenic variant: genomic-phenotypic analysis
JOURNAL J Med Life 17 (5), 500-507 (2024)
PUBMED 39144687
REMARK GeneRIF: MED23 pathogenic variant: genomic-phenotypic analysis.
REFERENCE 3 (bases 1 to 5083)
AUTHORS Zhang,N., Liu,X., Huang,L., Zeng,J., Ma,C., Han,L., Li,W., Yu,J.
and Yang,M.
TITLE LINC00921 reduces lung cancer radiosensitivity by destabilizing
NUDT21 and driving aberrant MED23 alternative polyadenylation
JOURNAL Cell Rep 42 (12), 113479 (2023)
PUBMED 37999979
REMARK GeneRIF: LINC00921 reduces lung cancer radiosensitivity by
destabilizing NUDT21 and driving aberrant MED23 alternative
polyadenylation.
REFERENCE 4 (bases 1 to 5083)
AUTHORS Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
and Leonetti,M.D.
TITLE OpenCell: Endogenous tagging for the cartography of human cellular
organization
JOURNAL Science 375 (6585), eabi6983 (2022)
PUBMED 35271311
REFERENCE 5 (bases 1 to 5083)
AUTHORS Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
Harper,J.W. and Gygi,S.P.
TITLE Dual proteome-scale networks reveal cell-specific remodeling of the
human interactome
JOURNAL Cell 184 (11), 3022-3040 (2021)
PUBMED 33961781
REFERENCE 6 (bases 1 to 5083)
AUTHORS Ko,L., Cardona,G.R. and Chin,W.W.
TITLE Thyroid hormone receptor-binding protein, an LXXLL motif-containing
protein, functions as a general coactivator
JOURNAL Proc Natl Acad Sci U S A 97 (11), 6212-6217 (2000)
PUBMED 10823961
REFERENCE 7 (bases 1 to 5083)
AUTHORS Boyer,T.G., Martin,M.E., Lees,E., Ricciardi,R.P. and Berk,A.J.
TITLE Mammalian Srb/Mediator complex is targeted by adenovirus E1A
protein
JOURNAL Nature 399 (6733), 276-279 (1999)
PUBMED 10353252
REFERENCE 8 (bases 1 to 5083)
AUTHORS Naar,A.M., Beaurang,P.A., Zhou,S., Abraham,S., Solomon,W. and
Tjian,R.
TITLE Composite co-activator ARC mediates chromatin-directed
transcriptional activation
JOURNAL Nature 398 (6730), 828-832 (1999)
PUBMED 10235267
REFERENCE 9 (bases 1 to 5083)
AUTHORS Rachez,C., Lemon,B.D., Suldan,Z., Bromleigh,V., Gamble,M.,
Naar,A.M., Erdjument-Bromage,H., Tempst,P. and Freedman,L.P.
TITLE Ligand-dependent transcription activation by nuclear receptors
requires the DRIP complex
JOURNAL Nature 398 (6730), 824-828 (1999)
PUBMED 10235266
REFERENCE 10 (bases 1 to 5083)
AUTHORS Ryu,S., Zhou,S., Ladurner,A.G. and Tjian,R.
TITLE The transcriptional cofactor complex CRSP is required for activity
of the enhancer-binding protein Sp1
JOURNAL Nature 397 (6718), 446-450 (1999)
PUBMED 9989412
COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from AL121575.24.
Summary: The activation of gene transcription is a multistep
process that is triggered by factors that recognize transcriptional
enhancer sites in DNA. These factors work with co-activators to
direct transcriptional initiation by the RNA polymerase II
apparatus. The protein encoded by this gene is a subunit of the
CRSP (cofactor required for SP1 activation) complex, which, along
with TFIID, is required for efficient activation by SP1. This
protein is also a component of other multisubunit complexes e.g.
thyroid hormone receptor-(TR-) associated proteins which interact
with TR and facilitate TR function on DNA templates in conjunction
with initiation factors and cofactors. This protein also acts as a
metastasis suppressor. Several alternatively spliced transcript
variants encoding different isoforms have been described for this
gene. [provided by RefSeq, Jul 2012].
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
[ECO:0006172]
##Evidence-Data-END##
COMPLETENESS: full length.
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-232 AL121575.24 128316-128547 c
233-264 AL121575.24 127946-127977 c
265-352 AL121575.24 127701-127788 c
353-477 AL121575.24 125170-125294 c
478-589 AL121575.24 123656-123767 c
590-688 AL121575.24 122186-122284 c
689-790 AL121575.24 120933-121034 c
791-860 AL121575.24 120132-120201 c
861-973 AL121575.24 118712-118824 c
974-1069 AL121575.24 116212-116307 c
1070-1087 AL121575.24 115629-115646 c
1088-1129 AL121575.24 110351-110392 c
1130-1273 AL121575.24 108233-108376 c
1274-1419 AL121575.24 106784-106929 c
1420-1665 AL121575.24 105545-105790 c
1666-1808 AL121575.24 104483-104625 c
1809-1983 AL121575.24 103335-103509 c
1984-2147 AL121575.24 102523-102686 c
2148-2272 AL121575.24 100343-100467 c
2273-2478 AL121575.24 98861-99066 c
2479-2659 AL121575.24 98592-98772 c
2660-2830 AL121575.24 96823-96993 c
2831-3047 AL121575.24 96252-96468 c
3048-3284 AL121575.24 94404-94640 c
3285-3450 AL121575.24 93311-93476 c
3451-3523 AL121575.24 92693-92765 c
3524-3738 AL121575.24 91618-91832 c
3739-3859 AL121575.24 90627-90747 c
3860-3991 AL121575.24 89770-89901 c
3992-5083 AL121575.24 87060-88151 c
FEATURES Location/Qualifiers
source 1..5083
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="6"
/map="6q23.2"
gene 1..5083
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/note="mediator complex subunit 23"
/db_xref="GeneID:9439"
/db_xref="HGNC:HGNC:2372"
/db_xref="MIM:605042"
exon 1..232
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
CDS 194..4159
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/note="isoform h is encoded by transcript variant 8; 133
kDa transcriptional co-activator; 130 kDa transcriptional
co-activator; vitamin D3 receptor interacting protein;
mediator of RNA polymerase II transcription subunit 23;
activator-recruited cofactor 130 kDa component; cofactor
required for Sp1 transcriptional activation subunit 3"
/codon_start=1
/product="mediator of RNA polymerase II transcription
subunit 23 isoform h"
/protein_id="NP_001363449.1"
/db_xref="GeneID:9439"
/db_xref="HGNC:HGNC:2372"
/db_xref="MIM:605042"
/translation="METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAF
RQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLI
NSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQL
LAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARI
NSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQ
PYSRDMVCNMLGLNKQTLNIAQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFIS
GSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQND
NSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVE
TIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKS
SVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMH
HIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSD
PKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWAS
HTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPP
LFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNK
CIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDF
VKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCL
RFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDR
AFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVD
TMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQP
LVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDF
TACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHL
VGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDS
VKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVPVSLPVT
Q"
exon 233..264
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 265..352
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 353..477
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 478..589
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 590..688
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 689..790
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 791..860
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 861..973
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 974..1069
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 1070..1087
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 1088..1129
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 1130..1273
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 1274..1419
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 1420..1665
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 1666..1808
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 1809..1983
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 1984..2147
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 2148..2272
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 2273..2478
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 2479..2659
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 2660..2830
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 2831..3047
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 3048..3284
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 3285..3450
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 3451..3523
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 3524..3738
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 3739..3859
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 3860..3991
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
exon 3992..5083
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/inference="alignment:Splign:2.1.0"
regulatory 5064..5069
/regulatory_class="polyA_signal_sequence"
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/note="hexamer: AGTAAA"
polyA_site 5083
/gene="MED23"
/gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
MRT18; SUR-2; SUR2"
/note="major polyA site"
ORIGIN
1 gtcttccgta caggttcctg ctgttggctg ggctcgggag ggaacgctcc aggtaaagcg
61 ctgggggagg aagcgacgcc gaggagctac ggtttcctcc agaggtctcc gcccctctgc
121 ccctatattc ccagagctcg agtctgatcc gggccttgcc gggcaccctg gaaaggcggg
181 ggtgatagta cagatggaga cgcaactgca gagcattttc gaagaggtgg tgaaaacgga
241 agttatagaa gaggcttttc ctggcatgtt tatggatact cctgaagatg agaaaacaaa
301 actaattagc tgtttggggg ccttcagaca gttttggggt ggtctttctc aggagtctca
361 tgaacagtgt atccagtgga ttgttaagtt tattcatggt cagcatagtc ctaaaagaat
421 ttcttttctt tatgactgct tagcaatggc agttgagact ggtctccttc cacccaggct
481 ggtttgtgaa tccctgataa actctgacac tcttgagtgg gaaagaacac agctttgggc
541 cttaacattt aaactggttc ggaaaataat tgggggagtg gattacaagg gtgttcgaga
601 tctcttaaaa gtgattttgg agaagatttt gacaattcct aatacagtga gctctgctgt
661 tgtacagcag cttctggcag caagagaggt tatagcatat atcttggaaa gaaatgcctg
721 cttattacca gcctattttg cagtcactga gatcaggaaa ctgtatcctg aaggcaaact
781 tccacactgg ttacttggaa acctagtatc agactttgtg gataccttca ggcccacagc
841 aaggataaac tccatttgtg gtcgctgtag tcttctgcca gttgtaaata attcgggtgc
901 catttgtaat tcatggaaac tggatcctgc tactcttcgt tttcctttga aaggcctttt
961 gccatatgat aaggatctgt ttgaaccaca gactgctttg ttgagatatg tattggagca
1021 gccttattcc agggatatgg tctgcaatat gctaggttta aataagcaga ccttgaacat
1081 tgctcagttt gcaagttttc cacatatggt gctttctctt catcagaagt tagcagggcg
1141 aggactgatt aaaggcagag atcatcttat gtgggttctc ctgcaattca tttctggaag
1201 tattcagaaa aatgcactag ctgattttct ccctgtgatg aagctcttcg acttgctata
1261 cccagaaaaa gaatatatcc cagttcctga tattaacaaa ccccagtcaa cccatgcctt
1321 tgcaatgacc tgtatttgga ttcatctcaa tagaaaagct caaaatgaca actccaagct
1381 acagattcca atacctcatt ccctaagact tcaccatgag ttcctgcagc agagtctaag
1441 aaataaaagt ttacagatga atgactataa gattgctcta ttgtgtaatg catactctac
1501 aaattcagaa tgttttacat tacccatggg agctctggta gaaactattt atggaaatgg
1561 aattatgagg atacctctcc ctggaacaaa ctgtatggct tcaggatcta ttaccccctt
1621 acctatgaac ctcctggatt cactgacagt tcatgccaaa atgagcctta ttcacagcat
1681 tgcaaccagg gtgataaaac ttgctcatgc aaagtccagt gtggccttgg ctccagccct
1741 agtggaaact tacagtcgtt tattggtcta tatggaaata gagtctttgg gcatcaaagg
1801 atttatcagt cagcttttgc caactgtttt caaatcacat gcatggggga tcttacacac
1861 actccttgag atgtttagct accggatgca tcatattcag cctcattaca gagttcagct
1921 cctgagtcat cttcatactt tggctgcagt tgcacaaaca aaccagaacc agctccatct
1981 ttgtgtcgag agcactgctc tcaggcttat aacagcatta ggtagctcag aggtacaacc
2041 gcagtttaca cgcttcctta gtgatcccaa aacagtgctc tcagcagaat ctgaagaact
2101 gaaccgagcc ttgatattga ccttggctag agcaactcat gtaacagatt tttttacagg
2161 ctctgattca attcagggaa cttggtgtaa agacatactt cagaccatca tgagtttcac
2221 tcctcataat tgggcttcac acaccctgag ctgttttcca ggcccactac aggcattctt
2281 caaacaaaat aatgtgcctc aggaaagccg ttttaatctg aaaaaaaatg tggaggagga
2341 gtataggaag tggaagtcaa tgagcaacga aaacgacatt attacccact tctctatgca
2401 gggctcccct cctctctttc tttgtcttct ctggaaaatg ctcttggaaa cagatcatat
2461 taatcagatt ggctatagag tattagagag aattggagcc agggccttgg tagcccatgt
2521 gaggacattt gcagatttcc tggtatatga gttttctaca tcagcagggg gtcagcaact
2581 caataaatgc attgaaattc ttaatgacat ggtatggaag tataacattg ttacactgga
2641 cagattaatt ctctgcctgg ccatgcgtag tcacgaagga aatgaagccc aggtttgtta
2701 tttcataatt cagttgctgt tactcaaacc aaacgatttt agaaatcgag taagtgactt
2761 tgtgaaggaa aattccccag agcactggtt acagaatgac tggcacacca agcacatgaa
2821 ttatcacaag aaatatccag agaagttgta ttttgagggc ctcgcggaac aggtggatcc
2881 tcctgtacag atccagtctc cctatctgcc catctatttt gggaatgtgt gtcttcgatt
2941 ccttccagta tttgatatag taatccacag atttttagag ttgcttccgg tatccaaatc
3001 actggagact ctactggatc atctaggagg cttatataaa tttcatgatc gtccagtgac
3061 ttatctgtat aacactctgc actattatga aatgcacctg agagaccgcg catttctcaa
3121 acgaaaactc gtccatgcga tcattggctc tctgaaggat aatcgaccgc agggctggtg
3181 tctaagtgac acttacctga aatgcgctat gaatgcacga gaggaaaatc cttgggttcc
3241 agatgacacc tactattgca gattgattgg cagactagtc gatacgatgg ctggcaaatc
3301 tcctggtccc tttccaaact gtgactggag attcaatgag tttcccaacc cagctgccca
3361 tgctctccat gttacttgtg tggagctcat ggccttggca gtttcaggca aagaagttgg
3421 gaatgccctt ctaaatgttg tcctaaaaag tcagccttta gtgccaagag agaacattac
3481 agcatggatg aatgcaattg gtttgatcat cactgcccta ccagagccat attggattgt
3541 tcttcatgat cgaattgtga gtgtcatcag cagccccagc ttgacgtctg aaacagagtg
3601 ggttggctat ccattccgcc tctttgattt cactgcctgt catcagtcct actctgagat
3661 gagttgtagc tatacgttag ctcttgcaca tgctgtgtgg caccattcta gcatcggaca
3721 actttctctc attccaaagt ttcttactga agtacttctt cctatagtga agaccgaatt
3781 ccagttgctt tatgtatacc atcttgttgg accattttta caaagatttc agcaagagag
3841 aactcgttgt atgatagaga ttggtgtggc gttttatgac atgctgctga atgttgacca
3901 gtgtagcacc catttaaatt acatggatcc catctgtgac ttcctctatc acatgaagta
3961 tatgtttact ggtgacagcg tgaaagagca agtagagaag attatctgta acttaaaacc
4021 agctttaaaa cttcgtcttc gattcatcac acacattagc aagatggagc cagctgcagt
4081 gcctccacaa gccatgaaca gtgggtctcc agcacctcag tctaatcagg tgcccgtgtc
4141 tttaccagta actcagtgaa gccagactgt actgtggaga aagtagaaat atatccgtct
4201 ttgagagtgg actcaaacct tttaaatctg aatgcgatca cactgtagtg atatagtaga
4261 agcagtgatg atattcagat tgttttattt tgattcaaat gatgaatctg ttgacacctt
4321 ataactccca tctccctatc tatatttttg tctctaagag atcaagtaag atacatgttg
4381 ttcatattca taacaactta ttagagctat gtaggaacat agaggtatct gtgatcaaat
4441 gacttgtcat tggcactttc agtctttcct attctagccg ttgtttttgt ttgtattttt
4501 tattattaat gacatattta gtcttcagat atattatttt aaaggcacca cacatgtaaa
4561 acagttatta caaatatttt atacataaat ctcttggtag attttcagaa taatagagat
4621 ttatatcaag aaaaggaatc tagattttgt tgcattgtag gagcaaacaa ggcttcatat
4681 tctttttaaa atgacttgaa attttattat aaatttccat agtttctatc aaggtcaact
4741 ggcagtttta ttacctttgc tatggacttg aacttactgt ataaaattag ttggaagcca
4801 tgtaagaaaa taacatctga aaattcttga acttaaaatc atgcttaaat ggttgattct
4861 ccaatttctt tttttaaaaa taagttaatt gcttttgcag gttgacactc tcacctgaca
4921 gatgatgtaa ttcttcaatt tttataatct taaaattttt aaattttata tttgtaaata
4981 cagtacacat tttatttctt ggattttgag agacattgtt aattttgggg gaattggcat
5041 tgcgaaagac ttgaaaacta atgagtaaag tctgctgaat gaa
//