LOCUS NM_001412371 2570 bp mRNA linear ROD 12-JUN-2024
DEFINITION Mus musculus epsin 1 (Epn1), transcript variant 38, mRNA.
ACCESSION NM_001412371 XM_030242113
VERSION NM_001412371.1
KEYWORDS RefSeq.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 2570)
AUTHORS Dong,Y., Wang,B., Cui,K., Cai,X., Bhattacharjee,S., Wong,S.,
Cowan,D.B. and Chen,H.
TITLE Epsins Negatively Regulate Aortic Endothelial Cell Function by
Augmenting Inflammatory Signaling
JOURNAL Cells 10 (8), 1918 (2021)
PUBMED 34440686
REMARK GeneRIF: Epsins Negatively Regulate Aortic Endothelial Cell
Function by Augmenting Inflammatory Signaling.
Publication Status: Online-Only
REFERENCE 2 (bases 1 to 2570)
AUTHORS Song,K., Cai,X., Dong,Y., Wu,H., Wei,Y., Shankavaram,U.T., Cui,K.,
Lee,Y., Zhu,B., Bhattacharjee,S., Wang,B., Zhang,K., Wen,A.,
Wong,S., Yu,L., Xia,L., Welm,A.L., Bielenberg,D.R.,
Camphausen,K.A., Kang,Y. and Chen,H.
TITLE Epsins 1 and 2 promote NEMO linear ubiquitination via LUBAC to
drive breast cancer development
JOURNAL J Clin Invest 131 (1) (2021)
PUBMED 32960814
REMARK GeneRIF: Epsins 1 and 2 promote NEMO linear ubiquitination via
LUBAC to drive breast cancer development.
REFERENCE 3 (bases 1 to 2570)
AUTHORS Dong,Y., Lee,Y., Cui,K., He,M., Wang,B., Bhattacharjee,S., Zhu,B.,
Yago,T., Zhang,K., Deng,L., Ouyang,K., Wen,A., Cowan,D.B., Song,K.,
Yu,L., Brophy,M.L., Liu,X., Wylie-Sears,J., Wu,H., Wong,S., Cui,G.,
Kawashima,Y., Matsumoto,H., Kodera,Y., Wojcikiewicz,R.J.H.,
Srivastava,S., Bischoff,J., Wang,D.Z., Ley,K. and Chen,H.
TITLE Epsin-mediated degradation of IP3R1 fuels atherosclerosis
JOURNAL Nat Commun 11 (1), 3984 (2020)
PUBMED 32770009
REMARK GeneRIF: Epsin-mediated degradation of IP3R1 fuels atherosclerosis.
Publication Status: Online-Only
REFERENCE 4 (bases 1 to 2570)
AUTHORS Milesi,C., Alberici,P., Pozzi,B., Oldani,A., Beznoussenko,G.V.,
Raimondi,A., Soppo,B.E., Amodio,S., Caldieri,G., Malabarba,M.G.,
Bertalot,G., Confalonieri,S., Parazzoli,D., Mironov,A.A.,
Tacchetti,C., Di Fiore,P.P., Sigismund,S. and Offenhauser,N.
TITLE Redundant and nonredundant organismal functions of EPS15 and
EPS15L1
JOURNAL Life Sci Alliance 2 (1), e201800273 (2019)
PUBMED 30692166
REMARK Publication Status: Online-Only
REFERENCE 5 (bases 1 to 2570)
AUTHORS Wu,H., Rahman,H.N.A., Dong,Y., Liu,X., Lee,Y., Wen,A., To,K.H.,
Xiao,L., Birsner,A.E., Bazinet,L., Wong,S., Song,K., Brophy,M.L.,
Mahamud,M.R., Chang,B., Cai,X., Pasula,S., Kwak,S., Yang,W.,
Bischoff,J., Xu,J., Bielenberg,D.R., Dixon,J.B., D'Amato,R.J.,
Srinivasan,R.S. and Chen,H.
TITLE Epsin deficiency promotes lymphangiogenesis through regulation of
VEGFR3 degradation in diabetes
JOURNAL J Clin Invest 128 (9), 4025-4043 (2018)
PUBMED 30102256
REMARK GeneRIF: Diabetes attenuated VEGF-C-induced lymphangiogenesis in
corneal micropocket and Matrigel plug assays in WT mice but not in
mice with inducible lymphatic-specific deficiency of epsins 1 and 2
(LEC-iDKO).
REFERENCE 6 (bases 1 to 2570)
AUTHORS Pasula,S., Cai,X., Dong,Y., Messa,M., McManus,J., Chang,B., Liu,X.,
Zhu,H., Mansat,R.S., Yoon,S.J., Hahn,S., Keeling,J., Saunders,D.,
Ko,G., Knight,J., Newton,G., Luscinskas,F., Sun,X., Towner,R.,
Lupu,F., Xia,L., Cremona,O., De Camilli,P., Min,W. and Chen,H.
TITLE Endothelial epsin deficiency decreases tumor growth by enhancing
VEGF signaling
JOURNAL J Clin Invest 122 (12), 4424-4438 (2012)
PUBMED 23187125
REMARK GeneRIF: Loss of epsins 1 and 2 impaired endocytosis and
degradation of VEGFR2, resulting in excessive VEGF signaling and
compromised tumor vascular function.
REFERENCE 7 (bases 1 to 2570)
AUTHORS Chen,H., Ko,G., Zatti,A., Di Giacomo,G., Liu,L., Raiteri,E.,
Perucco,E., Collesi,C., Min,W., Zeiss,C., De Camilli,P. and
Cremona,O.
TITLE Embryonic arrest at midgestation and disruption of Notch signaling
produced by the absence of both epsin 1 and epsin 2 in mice
JOURNAL Proc Natl Acad Sci U S A 106 (33), 13838-13843 (2009)
PUBMED 19666558
REMARK GeneRIF: epsin has a role as a specialized endocytic adaptor, with
a critical role in the activation of Notch signaling in mammals
REFERENCE 8 (bases 1 to 2570)
AUTHORS Wang,H., Traub,L.M., Weixel,K.M., Hawryluk,M.J., Shah,N.,
Edinger,R.S., Perry,C.J., Kester,L., Butterworth,M.B., Peters,K.W.,
Kleyman,T.R., Frizzell,R.A. and Johnson,J.P.
TITLE Clathrin-mediated endocytosis of the epithelial sodium channel.
Role of epsin
JOURNAL J Biol Chem 281 (20), 14129-14135 (2006)
PUBMED 16574660
REMARK GeneRIF: epsin is an accessory protein linking ENaC to the
clathrin-based endocytic machinery thereby regulating the activity
of this ion channel at the cell surface
REFERENCE 9 (bases 1 to 2570)
AUTHORS Chen,H., Slepnev,V.I., Di Fiore,P.P. and De Camilli,P.
TITLE The interaction of epsin and Eps15 with the clathrin adaptor AP-2
is inhibited by mitotic phosphorylation and enhanced by
stimulation-dependent dephosphorylation in nerve terminals
JOURNAL J Biol Chem 274 (6), 3257-3260 (1999)
PUBMED 9920862
REFERENCE 10 (bases 1 to 2570)
AUTHORS Yamabhai,M., Hoffman,N.G., Hardison,N.L., McPherson,P.S.,
Castagnoli,L., Cesareni,G. and Kay,B.K.
TITLE Intersectin, a novel adaptor protein with two Eps15 homology and
five Src homology 3 domains
JOURNAL J Biol Chem 273 (47), 31401-31407 (1998)
PUBMED 9813051
COMMENT VALIDATED REFSEQ: This record has undergone validation or
preliminary review. The reference sequence was derived from
AC162893.6.
On Sep 29, 2022 this sequence version replaced XM_030242113.2.
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: SRR7345562.1959776.1,
SRR7652917.1056212.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns
SAMN00849374, SAMN00849375
[ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: full length.
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-390 AC162893.6 47117-47506
391-494 AC162893.6 48886-48989
495-747 AC162893.6 50598-50850
748-997 AC162893.6 56787-57036
998-1122 AC162893.6 59709-59833
1123-1206 AC162893.6 60767-60850
1207-1510 AC162893.6 61822-62125
1511-1621 AC162893.6 62511-62621
1622-1705 AC162893.6 62759-62842
1706-1963 AC162893.6 64022-64279
1964-2570 AC162893.6 64440-65046
FEATURES Location/Qualifiers
source 1..2570
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6"
/db_xref="taxon:10090"
/chromosome="7"
/map="7 2.94 cM"
gene 1..2570
/gene="Epn1"
/gene_synonym="Ibp1"
/note="epsin 1"
/db_xref="GeneID:13854"
/db_xref="MGI:MGI:1333763"
exon 1..390
/gene="Epn1"
/gene_synonym="Ibp1"
/inference="alignment:Splign:2.1.0"
exon 391..494
/gene="Epn1"
/gene_synonym="Ibp1"
/inference="alignment:Splign:2.1.0"
misc_feature 403..405
/gene="Epn1"
/gene_synonym="Ibp1"
/note="upstream in-frame stop codon"
exon 495..747
/gene="Epn1"
/gene_synonym="Ibp1"
/inference="alignment:Splign:2.1.0"
CDS 520..2172
/gene="Epn1"
/gene_synonym="Ibp1"
/note="isoform 7 is encoded by transcript variant 38;
EPS-15-interacting protein 1; intersectin-EH-binding
protein 1"
/codon_start=1
/product="epsin-1 isoform 7"
/protein_id="NP_001399300.1"
/db_xref="GeneID:13854"
/db_xref="MGI:MGI:1333763"
/translation="MSTSSLRRQMKNIVHNYSEAEIKVREATSNDPWGPSSSLMSEIA
DLTYNVVAFSEIMSMIWKRLNDHGKNWRHVYKAMTLMEYLIKTGSERVSQQCKENMYA
VQTLKDFQYVDRDGKDQGVNVREKAKQLVALLRDEDRLREERAHALKTKEKLAQTATA
SSAAVGSGPPPEAEQAWPQSSGEEELQLQLALAMSKEEADQEERIRRGDDLRLQMAIE
ESKRETGGKEESSLMDLADVFTTPAPPQASDPWGGPASVPTAVPVAAAASDPWGGPAV
PPAADPWGGAAPTPASGDPWRPAAPTGPSVDPWGGTPAPAAGEGPTPDPWGSSDGGAP
VSGPPSSDPWAPAPAFSDPWGGSPAKPSSNGTAVGGFDTEPDEFSDFDRLRTALPTSG
SSTGELELLAGEVPARSPGAFDMSGVGGSLAESVGSPPPAATPTPTPPTRKTPESFLG
PNAALVDLDSLVSRPGPTPPGSKASNPFLPSGAPPTGPSVTNPFQPAPPATLTLNQLR
LSPVPPVPGAPPTYISPLGGGPGLPPMMPPGPPAPNTNPFLL"
exon 748..997
/gene="Epn1"
/gene_synonym="Ibp1"
/inference="alignment:Splign:2.1.0"
exon 998..1122
/gene="Epn1"
/gene_synonym="Ibp1"
/inference="alignment:Splign:2.1.0"
exon 1123..1206
/gene="Epn1"
/gene_synonym="Ibp1"
/inference="alignment:Splign:2.1.0"
exon 1207..1510
/gene="Epn1"
/gene_synonym="Ibp1"
/inference="alignment:Splign:2.1.0"
exon 1511..1621
/gene="Epn1"
/gene_synonym="Ibp1"
/inference="alignment:Splign:2.1.0"
exon 1622..1705
/gene="Epn1"
/gene_synonym="Ibp1"
/inference="alignment:Splign:2.1.0"
exon 1706..1963
/gene="Epn1"
/gene_synonym="Ibp1"
/inference="alignment:Splign:2.1.0"
exon 1964..2570
/gene="Epn1"
/gene_synonym="Ibp1"
/inference="alignment:Splign:2.1.0"
regulatory 2551..2556
/regulatory_class="polyA_signal_sequence"
/gene="Epn1"
/gene_synonym="Ibp1"
/note="hexamer: AATAAA"
polyA_site 2570
/gene="Epn1"
/gene_synonym="Ibp1"
/note="major polyA site"
ORIGIN
1 ggttgcttcc cgtccgctcg gcggctcccc tcccccgccc ggctctctgc gcccctcccg
61 ggcgccgggg cggcggggcc gtcgctgtgc ggccctccgt gcgtctgtgc gtgcgcacgt
121 gcgcacttct gcttcccgca gctcggcgcg cgccccgcga caccgaggcc gagcgggggc
181 agggggctga ccgtcatgac ccctggaagc ccggtataag cgggccgagg tgagcgaggg
241 gcgcaatgct ggcctccgtc gcggcaagtc cggtgttccg gcctgggccg tggcgcgcgc
301 gggtccagcc agcctcggag ccggtgttcc agccgctgat ggcttgtggc tgcttttgta
361 ccgctgtgcc cgggtcgggt ttgcctgccg gttggatcat ggtagggctc agtaaatctc
421 cctccatggc ttgttggaga ggcacagact ggtgatcgga gtctactctg gacccaggct
481 gctccagttg tgagcccctt gacaccacta ctgggcacaa tgtcgacctc atcgctgcgg
541 cgtcagatga agaatatcgt ccacaactac tcagaggctg agatcaaggt ccgcgaggcc
601 acaagcaatg atccctgggg cccatccagc tccctcatgt cagagattgc tgacctcacc
661 tacaatgttg tggccttctc cgagatcatg agcatgatct ggaagcggct caatgaccac
721 ggcaagaact ggaggcacgt ctacaaggcc atgacgttga tggagtacct catcaagact
781 ggctctgagc gtgtatcaca acagtgcaaa gagaacatgt atgctgtgca gacgctgaag
841 gacttccagt atgtggaccg tgatggcaag gaccagggtg tgaatgtgcg ggagaaggcc
901 aagcagctgg tggctttgct gcgtgatgag gatcggctcc gggaggagcg cgcccacgcg
961 ctcaagacca aggagaagct ggcacagact gccacggcct cctcagcagc cgtgggctct
1021 ggaccacctc ccgaggcgga gcaggcatgg ccacagagca gcggggagga ggagttgcag
1081 ctccagctgg ccctggctat gagcaaggag gaggccgacc aggaagagcg gatccgtcgt
1141 ggggatgacc tgaggctgca gatggcaata gaggagagca agagggagac tgggggcaag
1201 gaggagtcat ctctcatgga tcttgctgat gtcttcacaa ccccagcgcc tccacaggcc
1261 tcagaccctt gggggggccc ggcatctgtg cctactgctg tccctgtggc tgctgccgct
1321 tcagaccctt gggggggacc tgctgtccct ccagctgctg atccttgggg tggtgcagcc
1381 cccacaccag cctcagggga tccctggagg cctgcagccc ctacagggcc ctctgttgat
1441 ccttggggtg ggaccccagc tcctgcagct ggagaggggc ccacccctga cccgtgggga
1501 agctctgatg gtggggctcc tgtcagtggg cccccatctt ctgacccctg ggcacctgcc
1561 ccggctttct cagacccctg gggaggctca cctgcaaagc ccagcagcaa cggcactgca
1621 gttgggggct ttgacacaga gcctgatgag ttctcagact tcgaccgact ccgcactgcc
1681 ctgccaacct ctgggagcag cacgggggag ttggagttgc tcgcaggaga ggtgcctgct
1741 cgcagccctg gggcattcga catgagtggg gttgggggat ctctggctga gtctgtgggg
1801 agtcccccac ctgctgcaac cccaactccc acccccccaa cacggaagac tccggagtca
1861 ttcctaggcc ctaatgcagc ccttgtggat ctggactcgc tggtgagccg accaggcccc
1921 acaccaccag gatccaaggc ttccaaccca ttccttccaa gcggagctcc gcccacaggc
1981 ccctctgtca ccaatccttt ccagccagca ccccctgcaa cgctcaccct gaaccagctc
2041 cgtctcagtc ctgtgccccc agttcctgga gcgccaccca cctacatctc tcctcttggt
2101 ggaggccctg gcctgccccc catgatgccc ccaggtcccc cagcccccaa cactaacccc
2161 ttcctcctat aatccagggt aggagggagg cctggttcag ctggcttccc caatttctgc
2221 tccctgggag atcagtattg tgagtgcatg tgaaatgggg gggccctccc ccacagtgcc
2281 cttccccctc ctggggccca ctcacactac accctcttcc caagtcccca ccccacctcc
2341 ccttgagaaa aactggacat ggggatgggg aggggagccg gccagaggag gacccttttc
2401 tgtggcatta gatgggggag ggacagctgg ggttccccca cccattccac ctccctctaa
2461 actcatgaca acccccaaat cagtgtttga gcctcctcgt tcccttggca cccctggtga
2521 atccttggtg atgattttgg caacttcggg aataaatggc aattctcatg
//