LOCUS NM_002972 8019 bp mRNA linear PRI 08-OCT-2024
DEFINITION Homo sapiens SET binding factor 1 (SBF1), transcript variant 1,
mRNA.
ACCESSION NM_002972 NM_197971 XM_037447
VERSION NM_002972.4
KEYWORDS RefSeq; MANE Select.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 8019)
AUTHORS Khamse,S., Alizadeh,S., Bernhart,S.H., Afshar,H., Delbari,A. and
Ohadi,M.
TITLE A (GCC) repeat in SBF1 reveals a novel biological phenomenon in
human and links to late onset neurocognitive disorder
JOURNAL Sci Rep 12 (1), 15480 (2022)
PUBMED 36104480
REMARK GeneRIF: A (GCC) repeat in SBF1 reveals a novel biological
phenomenon in human and links to late onset neurocognitive
disorder.
Publication Status: Online-Only
REFERENCE 2 (bases 1 to 8019)
AUTHORS Gang,Q., Bettencourt,C., Holton,J., Lovejoy,C., Chelban,V.,
Oconnor,E., Yuan,Y., Reilly,M.M., Hanna,M. and Houlden,H.
TITLE A novel frameshift deletion in autosomal recessive SBF1-related
syndromic neuropathy with necklace fibres
JOURNAL J Neurol 267 (9), 2705-2712 (2020)
PUBMED 32444983
REMARK GeneRIF: A novel frameshift deletion in autosomal recessive
SBF1-related syndromic neuropathy with necklace fibres.
REFERENCE 3 (bases 1 to 8019)
AUTHORS Flusser,H., Halperin,D., Kadir,R., Shorer,Z., Shelef,I. and
Birk,O.S.
TITLE Novel SBF1 splice-site null mutation broadens the clinical spectrum
of Charcot-Marie-Tooth type 4B3 disease
JOURNAL Clin Genet 94 (5), 473-479 (2018)
PUBMED 30039846
REMARK GeneRIF: The novel SBF1 null mutation highlights distinct severe
phenotypic manifestations, broadening the clinical spectrum of
SBF1-related neuropathies: cerebellar and pyramidal signs evident
in the first months of life with peripheral polyneuropathy emerging
only toward the end of the first decade, together with unique MRI
findings.
REFERENCE 4 (bases 1 to 8019)
AUTHORS Manole,A., Horga,A., Gamez,J., Raguer,N., Salvado,M., San
Millan,B., Navarro,C., Pittmann,A., Reilly,M.M. and Houlden,H.
TITLE SBF1 mutations associated with autosomal recessive axonal
neuropathy with cranial nerve involvement
JOURNAL Neurogenetics 18 (1), 63-67 (2017)
PUBMED 28005197
REMARK GeneRIF: Novel sequence variants in SBF1 (c.1168C>G and
c.2209_2210del) as the potential causative mutations in two
siblings with severe axonal neuropathy, hearing loss, facial
weakness and bulbar features.
REFERENCE 5 (bases 1 to 8019)
AUTHORS Zhu,M.H., Ji,S.L., Zhang,C.Y., Cui,L., Xiong,L. and Zheng,H.L.
TITLE DNA microarray reveals ZNF195 and SBF1 are potential biomarkers for
gemcitabine sensitivity in head and neck squamous cell carcinoma
cell lines
JOURNAL Int J Clin Exp Pathol 7 (4), 1514-1523 (2014)
PUBMED 24817947
REMARK GeneRIF: ZNF195 and SBF1 are potential biomarkers for gemcitabine
sensitivity in head and neck squamous cell carcinoma cell lines.
Publication Status: Online-Only
REFERENCE 6 (bases 1 to 8019)
AUTHORS Firestein,R. and Cleary,M.L.
TITLE Pseudo-phosphatase Sbf1 contains an N-terminal GEF homology domain
that modulates its growth regulatory properties
JOURNAL J Cell Sci 114 (Pt 16), 2921-2927 (2001)
PUBMED 11686296
REFERENCE 7 (bases 1 to 8019)
AUTHORS Firestein,R., Cui,X., Huie,P. and Cleary,M.L.
TITLE Set domain-dependent regulation of transcriptional silencing and
growth control by SUV39H1, a mammalian ortholog of Drosophila
Su(var)3-9
JOURNAL Mol Cell Biol 20 (13), 4900-4909 (2000)
PUBMED 10848615
REFERENCE 8 (bases 1 to 8019)
AUTHORS Laporte,J., Blondeau,F., Buj-Bello,A., Tentler,D., Kretz,C.,
Dahl,N. and Mandel,J.L.
TITLE Characterization of the myotubularin dual specificity phosphatase
gene family from yeast to human
JOURNAL Hum Mol Genet 7 (11), 1703-1712 (1998)
PUBMED 9736772
REMARK GeneRIF: SBF1 (MTMR5) belongs to the myotubularin family of
phosphoinositides phosphatases-like
REFERENCE 9 (bases 1 to 8019)
AUTHORS Cui,X., De Vivo,I., Slany,R., Miyamoto,A., Firestein,R. and
Cleary,M.L.
TITLE Association of SET domain and myotubularin-related proteins
modulates growth control
JOURNAL Nat Genet 18 (4), 331-337 (1998)
PUBMED 9537414
REFERENCE 10 (bases 1 to 8019)
AUTHORS Bird,T.D.
TITLE Charcot-Marie-Tooth Neuropathy Type 4 - RETIRED CHAPTER, FOR
HISTORICAL REFERENCE ONLY
JOURNAL (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
Amemiya A (Eds.);
GENEREVIEWS(R);
(1993)
PUBMED 20301641
COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from HY035781.1, BC087612.1,
AL096767.14 and BM975530.1.
This sequence is a reference standard in the RefSeqGene project.
On Nov 22, 2018 this sequence version replaced NM_002972.3.
Summary: This gene encodes a member of the protein-tyrosine
phosphatase family. However, the encoded protein does not appear to
be a catalytically active phosphatase because it lacks several
amino acids in the catalytic pocket. This protein contains a
Guanine nucleotide exchange factor (GEF) domain which is necessary
for its role in growth and differentiation. Mutations in this gene
have been associated with Charcot-Marie-Tooth disease 4B3.
Pseudogenes of this gene have been defined on chromosomes 1 and 8.
[provided by RefSeq, Dec 2014].
Sequence Note: This RefSeq record was created from transcript and
genomic sequence data to make the sequence consistent with the
reference genome assembly. The genomic coordinates used for the
transcript record were based on transcript alignments.
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: BC087612.1, SRR14038194.2905869.1
[ECO:0000332]
RNAseq introns :: mixed sample support SAMEA1965299,
SAMEA1966682 [ECO:0006172]
##Evidence-Data-END##
##RefSeq-Attributes-START##
MANE Ensembl match :: ENST00000380817.8/ ENSP00000370196.2
RefSeq Select criteria :: based on conservation, expression
##RefSeq-Attributes-END##
COMPLETENESS: full length.
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-25 HY035781.1 10-34
26-6024 BC087612.1 1-5999
6025-7720 AL096767.14 20728-22423 c
7721-8019 BM975530.1 15-313 c
FEATURES Location/Qualifiers
source 1..8019
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="22"
/map="22q13.33"
gene 1..8019
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/note="SET binding factor 1"
/db_xref="GeneID:6305"
/db_xref="HGNC:HGNC:10542"
/db_xref="MIM:603560"
exon 1..250
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
CDS 196..5877
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/note="isoform 1 is encoded by transcript variant 1;
myotubularin-related protein 5; DENN/MADD domain
containing 7A; inactive phosphatidylinositol 3-phosphatase
5"
/codon_start=1
/product="myotubularin-related protein 5 isoform 1"
/protein_id="NP_002963.2"
/db_xref="CCDS:CCDS14091.2"
/db_xref="GeneID:6305"
/db_xref="HGNC:HGNC:10542"
/db_xref="MIM:603560"
/translation="MARLADYFVLVAFGPHPRGSGEGQGQILQRFPEKDWEDNPFPQG
IELFCQPSGWQLCPERNPPTFFVAVLTDINSERHYCACLTFWEPAEPSQETTRVEDAT
EREEEGDEGGQTHLSPTAPAPSAQLFAPKTLVLVSRLDHTEVFRNSLGLIYAIHVEGL
NVCLENVIGNLLTCTVPLAGGSQRTISLGAGDRQVIQTPLADSLPVSRCSVALLFRQL
GITNVLSLFCAALTEHKVLFLSRSYQRLADACRGLLALLFPLRYSFTYVPILPAQLLE
VLSTPTPFIIGVNAAFQAETQELLDVIVADLDGGTVTIPECVHIPPLPEPLQSQTHSV
LSMVLDPELELADLAFPPPTTSTSSLKMQDKELRAVFLRLFAQLLQGYRWCLHVVRIH
PEPVIRFHKAAFLGQRGLVEDDFLMKVLEGMAFAGFVSERGVPYRPTDLFDELVAHEV
ARMRADENHPQRVLRHVQELAEQLYKNENPYPAVAMHKVQRPGESSHLRRVPRPFPRL
DEGTVQWIVDQAAAKMQGAPPAVKAERRTTVPSGPPMTAILERCSGLHVNSARRLEVV
RNCISYVFEGKMLEAKKLLPAVLRALKGRAARRCLAQELHLHVQQNRAVLDHQQFDFV
VRMMNCCLQDCTSLDEHGIAAALLPLVTAFCRKLSPGVTQFAYSCVQEHVVWSTPQFW
EAMFYGDVQTHIRALYLEPTEDLAPAQEVGEAPSQEDERSALDVASEQRRLWPTLSRE
KQQELVQKEESTVFSQAIHYANRMSYLLLPLDSSKSRLLRERAGLGDLESASNSLVTN
SMAGSVAESYDTESGFEDAETCDVAGAVVRFINRFVDKVCTESGVTSDHLKGLHVMVP
DIVQMHIETLEAVQRESRRLPPIQKPKLLRPRLLPGEECVLDGLRVYLLPDGREEGAG
GSAGGPALLPAEGAVFLTTYRVIFTGMPTDPLVGEQVVVRSFPVAALTKEKRISVQTP
VDQLLQDGLQLRSCTFQLLKMAFDEEVGSDSAELFRKQLHKLRYPPDIRATFAFTLGS
AHTPGRPPRVTKDKGPSLRTLSRNLVKNAKKTIGRQHVTRKKYNPPSWEHRGQPPPED
QEDEISVSEELEPSTLTPSSALKPSDRMTMSSLVERACCRDYQRLGLGTLSSSLSRAK
SEPFRISPVNRMYAICRSYPGLLIVPQSVQDNALQRVSRCYRQNRFPVVCWRSGRSKA
VLLRSGGLHGKGVVGLFKAQNAPSPGQSQADSSSLEQEKYLQAVVSSMPRYADASGRN
TLSGFSSAHMGSHVPSPRARVTTLSNPMAASASRRTAPRGKWGSVRTSGRSSGLGTDV
GSRLAGRDALAPPQANGGPPDPGFLRPQRAALYILGDKAQLKGVRSDPLQQWELVPIE
VFEARQVKASFKKLLKACVPGCPAAEPSPASFLRSLEDSEWLIQIHKLLQVSVLVVEL
LDSGSSVLVGLEDGWDITTQVVSLVQLLSDPFYRTLEGFRLLVEKEWLSFGHRFSHRG
AHTLAGQSSGFTPVFLQFLDCVHQVHLQFPMEFEFSQFYLKFLGYHHVSRRFRTFLLD
SDYERIELGLLYEEKGERRGQVPCRSVWEYVDRLSKRTPVFHNYMYAPEDAEVLRPYS
NVSNLKVWDFYTEETLAEGPPYDWELAQGPPEPPEEERSDGGAPQSRRRVVWPCYDSC
PRAQPDAISRLLEELQRLETELGQPAERWKDTWDRVKAAQRLEGRPDGRGTPSSLLVS
TAPHHRRSLGVYLQEGPVGSTLSLSLDSDQSSGSTTSGSRQAARRSTSTLYSQFQTAE
SENRSYEGTLYKKGAFMKPWKARWFVLDKTKHQLRYYDHRVDTECKGVIDLAEVEAVA
PGTPTMGAPKTVDEKAFFDVKTTRRVYNFCAQDVPSAQQWVDRIQSCLSDA"
misc_feature 3391..3540
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/note="propagated from UniProtKB/Swiss-Prot (O95248.4);
Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
misc_feature 3553..3555
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/note="Phosphoserine.
/evidence=ECO:0007744|PubMed:23186163; propagated from
UniProtKB/Swiss-Prot (O95248.4); phosphorylation site"
misc_feature 3604..3606
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/note="Phosphothreonine.
/evidence=ECO:0007744|PubMed:18669648,
ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569;
propagated from UniProtKB/Swiss-Prot (O95248.4);
phosphorylation site"
misc_feature 3859..3861
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/note="N6-methyllysine.
/evidence=ECO:0007744|PubMed:24129315; propagated from
UniProtKB/Swiss-Prot (O95248.4); methylation site"
misc_feature 5086..5163
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/note="propagated from UniProtKB/Swiss-Prot (O95248.4);
Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
misc_feature 5437..5541
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/note="propagated from UniProtKB/Swiss-Prot (O95248.4);
Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
misc_feature 5509..5511
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/note="Phosphoserine.
/evidence=ECO:0007744|PubMed:23186163; propagated from
UniProtKB/Swiss-Prot (O95248.4); phosphorylation site"
exon 251..336
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 337..474
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 475..633
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 634..744
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 745..850
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 851..983
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 984..1092
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 1093..1206
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 1207..1284
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 1285..1398
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 1399..1527
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 1528..1626
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 1627..1831
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 1832..1944
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 1945..2094
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 2095..2163
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 2164..2322
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 2323..2591
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 2592..2764
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 2765..2838
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 2839..3034
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 3035..3162
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 3163..3341
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 3342..3478
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 3479..3686
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 3687..3883
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 3884..4021
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 4022..4099
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 4100..4281
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 4282..4461
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 4462..4563
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 4564..4749
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 4750..4876
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 4877..5007
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 5008..5238
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 5239..5346
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 5347..5558
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 5559..5646
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 5647..5778
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
exon 5779..8019
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/inference="alignment:Splign:2.1.0"
regulatory 6192..6197
/regulatory_class="polyA_signal_sequence"
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/note="hexamer: TATAAA"
polyA_site 6213
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/note="major polyA site"
regulatory 7996..8001
/regulatory_class="polyA_signal_sequence"
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/note="hexamer: ATTAAA"
polyA_site 8019
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
ORIGIN
1 gggcgggccg gctggctggg aagatggcgg cgggaacctg ggccgccgcc gccgccgccg
61 ccgccgccgc ggagcgaacc aggggtgtcc ggggtgcgcg gtccagggcc ggggccgggc
121 catgagcgcg ccgtcctcga gtccccgagc cgcggagccc gcccgcgccc ctcgggccgc
181 cccgcgtccc tcgccatggc gcggctcgcg gactacttcg tgctggtggc gttcgggccg
241 cacccgcgcg ggagtgggga aggccagggc cagattctgc agcgcttccc agagaaggac
301 tgggaggaca acccattccc ccagggcatc gagctgtttt gccagcccag cgggtggcag
361 ctgtgtcccg agaggaatcc accgaccttc tttgttgctg tcctcaccga catcaactcc
421 gagcgccact actgcgcctg cttgaccttc tgggagccag cggagccttc acaggaaacg
481 acgcgcgtgg aggatgccac agagagggag gaagaggggg atgagggagg ccagacccac
541 ctgtctccca cagcacctgc cccatctgcc cagctgtttg caccgaagac gctggtactg
601 gtgtcgcgac tcgaccacac ggaggtgttc aggaacagcc ttggcctcat ctatgccatc
661 cacgtggagg gcctgaatgt gtgcctggag aacgtgattg ggaacctgct gacgtgcact
721 gtgcccctgg ctgggggctc gcagaggacg atctctttgg gggctggtga ccggcaggtc
781 atccagactc cactggccga ctcgctgccc gtcagccgct gcagcgtggc cctgctcttc
841 cgccagctag gcatcaccaa cgtgctgtct ttgttctgtg ccgccctcac ggagcacaag
901 gttctcttcc tgtcccggag ctaccagcgg ctcgccgatg cctgtagggg cctcctggca
961 ctgctgtttc ctctcagata cagcttcacc tatgtgccca tcctgccggc tcagctgctg
1021 gaggtcctca gcacacccac gcccttcatc attggggtca acgcggcctt ccaggcagag
1081 acccaggagc tgctcgatgt gattgttgct gatctggatg gagggacggt caccattcct
1141 gagtgtgtgc acattccacc cttgccagag ccactgcaga gtcagacgca cagtgtgctg
1201 agcatggtcc tggacccgga gctggagttg gctgacctcg ccttccctcc gcccacgaca
1261 tccacctcct ccctgaagat gcaggacaag gagctgcgcg cggtcttcct gcggctgttc
1321 gctcagctgc tgcagggcta tcgctggtgc ctgcacgtcg tgcgcatcca cccggagcct
1381 gtcatccgct tccataaggc agccttcctg ggccagcgtg ggctggtaga ggacgatttc
1441 ctgatgaagg tgctggaggg catggccttt gctggctttg tgtcagagcg tggggtccca
1501 taccgcccta cggacctgtt cgatgagctg gtggcccacg aggtggcaag gatgcgggcg
1561 gatgagaacc acccccagcg tgtcctgcgt cacgtccagg aactggcaga gcagctctac
1621 aagaacgaga acccgtaccc agccgtggcg atgcacaagg tacagaggcc cggtgagagc
1681 agccacctgc gacgggtgcc ccgacccttc ccccggctgg atgagggcac cgtgcagtgg
1741 atcgtggacc aggctgcagc caagatgcag ggtgcacccc cagctgtgaa ggccgagagg
1801 aggaccaccg tgccctcagg gccccccatg actgccatac tggagcggtg cagtgggctg
1861 catgtcaaca gcgcccggcg gctggaggtt gtgcgcaact gcatctccta cgtgtttgag
1921 gggaaaatgc ttgaggccaa gaagctgctc ccagccgtgt tgagggccct gaaggggcga
1981 gctgcccgcc gctgcctcgc ccaggagctg cacctgcatg tgcagcagaa ccgtgcggtc
2041 ctggaccacc agcagtttga ctttgtcgtc cgtatgatga actgctgcct gcaggactgc
2101 acttctctgg acgagcatgg cattgcggcg gctctgctgc ctctggtcac agccttctgc
2161 cggaagctga gcccgggggt gacgcagttt gcatacagct gtgtgcagga gcacgtggtg
2221 tggagcacgc cacagttctg ggaggccatg ttctatgggg atgtgcagac tcacatccgg
2281 gccctctacc tggagcccac ggaggacctg gcccccgccc aggaggttgg ggaggcacct
2341 tcccaggagg acgagcgctc tgccctagac gtggcttctg agcagcggcg cttgtggcca
2401 actctgagtc gtgagaagca gcaggagctg gtgcagaagg aggagagcac ggtgttcagc
2461 caggccatcc actatgccaa ccgcatgagc tacctcctcc tgcccctgga cagcagcaag
2521 agccgcctac ttcgggagcg tgccgggctg ggcgacctgg agagcgccag caacagcctg
2581 gtcaccaaca gcatggctgg cagtgtggcc gagagctatg acacggagag cggcttcgag
2641 gatgcagaga cctgcgacgt agctggggct gtggtccgct tcatcaaccg ctttgtggac
2701 aaggtctgca cggagagtgg ggtcaccagc gaccacctca aggggctgca tgtcatggtg
2761 ccagacattg tccagatgca catcgagacc ctggaggccg tgcagcggga gagccggagg
2821 ctgccgccca tccagaagcc caagctgctg cggccgcgcc tgctgccggg tgaggagtgt
2881 gtgctggacg gcctgcgcgt ctacctgctg ccggatgggc gtgaggaggg cgcggggggc
2941 agtgctgggg gaccagcatt gctcccagct gagggcgccg tcttcctcac cacgtaccgg
3001 gtcatcttca cggggatgcc cacggacccc ctggttgggg agcaggtggt ggtccgctcc
3061 ttcccggtgg ctgcgctgac caaggagaag cgcatcagcg tccagacccc tgtggaccag
3121 ctcctgcagg acgggctcca gctgcgctcc tgcacattcc agctgctgaa aatggccttt
3181 gacgaggagg tggggtctga cagcgccgag ctcttccgta agcagctgca taagctgcgg
3241 tacccgccgg acatcagggc cacctttgcg ttcaccttgg gctctgccca cacacctggc
3301 cggccaccgc gagtcaccaa ggacaagggt ccttccctca gaaccctgtc ccggaacctg
3361 gtcaagaacg ccaagaagac catcgggcgg cagcatgtca ctcgcaagaa gtacaacccc
3421 cccagctggg agcaccgggg ccagccgccc cctgaggacc aggaggacga gatctcagtg
3481 tcggaggagc tggagcccag cacgctgacc ccgtcctcag ccctgaagcc ctccgaccgc
3541 atgaccatga gcagcctggt ggaaagggct tgctgtcgcg actaccagcg cctcggtctg
3601 ggcaccctga gcagcagcct gagccgggcc aagtctgagc ccttccgcat ttctccggtc
3661 aaccgcatgt atgccatctg ccgcagctac ccagggctgc tgatcgtgcc ccagagtgtc
3721 caggacaacg ccctgcagcg cgtgtcccgc tgctaccgcc agaaccgctt ccccgtggtc
3781 tgctggcgca gcgggcggtc caaggcggtg ctgctgcgct ctggaggcct gcatggcaaa
3841 ggtgtcgtcg gcctcttcaa ggcccagaac gcaccttctc caggccagtc ccaggcggac
3901 tcgagtagcc tggagcagga gaagtacctg caggctgtgg tcagctccat gccccgctac
3961 gccgacgcgt cgggacgcaa cacgcttagc ggcttctcct cagcccacat gggcagtcac
4021 gttcccagcc ccagagccag ggtcaccacg ctgtccaacc ccatggcggc ctcggcctcc
4081 agacggaccg caccccgagg taagtggggc agtgtccgga ccagtggacg cagcagtggc
4141 cttggcaccg atgtgggctc ccggctagct ggcagagacg cgctggcccc accccaggcc
4201 aacgggggcc ctcccgaccc gggcttcctg cgtccgcagc gagcagccct ctatatcctt
4261 ggggacaaag cccagctcaa gggtgtgcgg tcagaccccc tgcagcagtg ggagctggtg
4321 cccattgagg tattcgaggc acggcaggtg aaggctagct tcaagaagct gctgaaagca
4381 tgtgtcccag gctgccccgc tgctgagccc agcccagcct ccttcctgcg ctcactggag
4441 gactcagagt ggctgatcca gatccacaag ctgctgcagg tgtctgtgct ggtggtggag
4501 ctcctggatt caggctcctc cgtgctggtg ggcctggagg atggctggga catcaccacc
4561 caggtggtat ccttggtgca gctgctctca gaccccttct accgcacgct ggagggcttt
4621 cgcctgctgg tggagaagga gtggctgtcc ttcggccatc gcttcagcca ccgtggagct
4681 cacaccctgg ccgggcagag cagcggcttc acacccgtct tcctgcagtt cctggactgc
4741 gtacaccagg tccacctgca gttccccatg gagtttgagt tcagccagtt ctacctcaag
4801 ttcctcggct accaccatgt gtcccgccgt ttccggacct tcctgctcga ctctgactat
4861 gagcgcattg agctggggct gctgtatgag gagaaggggg aacgcagggg ccaggtgccg
4921 tgcaggtctg tgtgggagta tgtggaccgg ctgagcaaga ggacgcctgt gttccacaat
4981 tacatgtatg cgcccgagga cgcagaggtc ctgcggccct acagcaacgt gtccaacctg
5041 aaggtgtggg acttctacac tgaggagacg ctggccgagg gccctcccta tgactgggaa
5101 ctggcccagg ggccccctga acccccagag gaagaacggt ctgatggagg cgctccccag
5161 agcaggcgcc gcgtggtgtg gccctgttac gacagctgcc cgcgggccca gcctgacgcc
5221 atctcacgcc tgctggagga gctgcagagg ctggagacag agttgggcca acccgctgag
5281 cgctggaagg acacctggga ccgggtgaag gctgcacagc gcctcgaggg ccggccagac
5341 ggccgtggca cccctagctc cctccttgtg tccaccgcac cccaccaccg tcgctcgctg
5401 ggtgtgtacc tgcaggaggg gcccgtgggc tccaccctga gcctcagcct ggacagcgac
5461 cagagtagtg gctcaaccac atccggctcc cgtcaggctg cccgccgcag caccagcacc
5521 ctgtacagcc agttccagac agcagagagt gagaacaggt cctacgaggg cactctgtac
5581 aagaaggggg ccttcatgaa gccttggaag gcccgctggt tcgtgctgga caagaccaag
5641 caccagctgc gctactacga ccaccgtgtg gacacagagt gcaagggtgt catcgacttg
5701 gcggaggtgg aggctgtggc acctggcacg cccactatgg gtgcccctaa gactgtggac
5761 gagaaggcct tctttgacgt gaagacaacg cgtcgcgttt acaacttctg tgcccaggac
5821 gtgccctcgg cccagcagtg ggtggaccgg atccagagct gcctgtcgga cgcctgagcc
5881 tcccagccct gcccggctgc tctgcttccg gtcgttaccg accactaggg gtgggcaggg
5941 ccgccccggc catgtttaca gccccggccc tcgacagtat tgaggccccg agcccccagc
6001 acttgtgtgt acagcccccg tccccgcccc gccccgcccg gccggcccta acttattttg
6061 gcgtcacagc tgagcaccgt gccgggaggt ggccaaggta cagcccgcaa tgggcctgta
6121 aatagtccgg ccccgtcagc gtgtgctggt ccagccagcg gctgcaggcg agtttctaga
6181 accagagtct atataaagag agaactaacg ccacgctcct gtgcctgcct tccccactcc
6241 ccggctgcct gctctcggcc tacccagagg gtcccatctg cccctatcca ggcccacctg
6301 gcgggaggtt ggcatctttc tcgtgagcct ctcctggtgc ctgggtccac ccagctcggc
6361 ctgcatgtcc ctgggagtga ctttgctctg ggggcggatc gagcaggagg cttcactggg
6421 gacttgcttg attccctcca cgcctcaggg ctggtctagg ggccggcacg gctggagagg
6481 aagcccccat ccctacccag gggatgcaga agctgacctc acagaggctt gggggtgaaa
6541 gggtgggtgg tcatttgacc ccagaaggct gttgcaggtc cagaggacac ttgaggtgga
6601 cgtcagtttc tggctagacc cgagctgaag ggatggaggc cggaggcggg gggggggggg
6661 ggacagtggg ctcccagggg aatgcaggtt gaccacatct ggctcctgcc aggcaacgag
6721 cagcatctgg cagagtaagg ggccaacgcc catgggggat ggaccctctc agttcttggg
6781 aattctgccc caaaagtcct ttccctgggg tctcagaggg cccccgtcct tcccttcttg
6841 gtgtcactgt ggcccctcac tgctcttttc ctattcaaac ctgagtccca ccaggcccag
6901 ggcttcacct gctgagctgt tgtgtccttg cctgtgacga ggcctggcca ggggtgcagg
6961 agcagaaggt ggggagggtt atagacgctg caaaggccaa gagaacatct gagagtggca
7021 gctggtgacc tggccagagg ggctggtgag gggcagagaa cctggctaga ggctgggtcc
7081 ctcaggtggt cctctcaggt gggaggcgag cagcaggtgt gggtgagggg aaggttctga
7141 tgacagctgc agaggcaggg cccagtgctg gcaggtgggg ggccaagacc ctcccctggt
7201 gggacgttga agccaaggat ggccttggac cctgtcaggc ccagcatggt cccgccacct
7261 cccccacccc acaggtggtg ttgggacacc tgggcgagat gtgagggtgg gctcacttga
7321 gccactgaaa ccagccaggt cttccctcag gccggacaga tggcgcctga ccgaagttcc
7381 tggcacctgg aaaacccaca ggtcagagta aggggagaaa ggaccctgcc ctccctgttc
7441 cacgtctgtg gggggagagg acaaatgcca ggcacagggt aggcggcgag aacaaggcac
7501 tcaatgtgta gctggggcag agactcggcc tctggggagc tgagcgggtt ccctccaccc
7561 ccaaccgtgg tggaaagaca agctcgctgg ggcggggtgg gggtctggtc tccacctgcc
7621 cctcccactc agccactgag gacaaggtgg ggcccaggct tctgggaggg ggagctggca
7681 caaaaggaag tcctggggtt gatgtgtttg agcgttaggc gaagtggttc cccccatccc
7741 ccaaacggaa aaatgtcagt atttgctaag ctgtagagac ctgatgccgt gatgtggcct
7801 gttccgcctc cacccattac acggggataa cgctgggggg tggcgggccc acaaaagagg
7861 tgctggagga gactctccca cccctggccg ggccggggct ttggggccgg aaggttcaca
7921 gtacgcggtt tgtccgaacg tcacggcttt tattgggagt tgggggtttg gggtgccctg
7981 tcaggtgatc agaacattaa aaatggactc aacgtaaaa
//