LOCUS NM_009661 3273 bp mRNA linear ROD 03-JUN-2024
DEFINITION Mus musculus arachidonate 8-lipoxygenase (Alox8), mRNA.
ACCESSION NM_009661 XM_126384
VERSION NM_009661.4
KEYWORDS RefSeq; RefSeq Select.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 3273)
AUTHORS Schafer,M., Reisch,F., Labuz,D., Machelska,H., Stehling,S.,
Puschel,G.P., Rothe,M., Heydeck,D. and Kuhn,H.
TITLE Humanization of the Reaction Specificity of Mouse Alox15b Inversely
Modified the Susceptibility of Corresponding Knock-In Mice in Two
Different Animal Inflammation Models
JOURNAL Int J Mol Sci 24 (13), 11034 (2023)
PUBMED 37446212
REMARK GeneRIF: Humanization of the Reaction Specificity of Mouse Alox15b
Inversely Modified the Susceptibility of Corresponding Knock-In
Mice in Two Different Animal Inflammation Models.
Publication Status: Online-Only
REFERENCE 2 (bases 1 to 3273)
AUTHORS Yli-Karjanmaa,M., Larsen,K.S., Fenger,C.D., Kristensen,L.K.,
Martin,N.A., Jensen,P.T., Breton,A., Nathanson,L., Nielsen,P.V.,
Lund,M.C., Carlsen,S.L., Gramsbergen,J.B., Finsen,B., Stubbe,J.,
Frich,L.H., Stolp,H., Brambilla,R., Anthony,D.C., Meyer,M. and
Lambertsen,K.L.
TITLE TNF deficiency causes alterations in the spatial organization of
neurogenic zones and alters the number of microglia and neurons in
the cerebral cortex
JOURNAL Brain Behav Immun 82, 279-297 (2019)
PUBMED 31505254
REFERENCE 3 (bases 1 to 3273)
AUTHORS Xiao,Y., Song,C., Lin,Q., Shi,X., Yu,W., Huang,X., Wang,H.,
Chen,Y., Wang,R., Geng,X., Qin,M., Hu,K., Fan,Y., Qiao,Y., Gao,E.,
Zhao,W. and Chang,J.
TITLE Cardioprotection of (+/-)-sodium 5-bromo-2-(alpha-hydroxypentyl)
benzoate (BZP) on mouse myocardium I/R injury through inhibiting
12/15-LOX-2 activity
JOURNAL J Mol Cell Cardiol 135, 52-66 (2019)
PUBMED 31362020
REMARK GeneRIF: Our findings, for the first time, strongly indicate that
1a may serve as a potent and promising cardioprotective agent in
treatment of ischemia-reperfusion (I/R) injury, at least partially
through targeting 12/15-LOX-2.
REFERENCE 4 (bases 1 to 3273)
AUTHORS van Eif,V.W.W., Stefanovic,S., van Duijvenboden,K., Bakker,M.,
Wakker,V., de Gier-de Vries,C., Zaffran,S., Verkerk,A.O.,
Boukens,B.J. and Christoffels,V.M.
TITLE Transcriptome analysis of mouse and human sinoatrial node cells
reveals a conserved genetic program
JOURNAL Development 146 (8) (2019)
PUBMED 30936179
REMARK Publication Status: Online-Only
REFERENCE 5 (bases 1 to 3273)
AUTHORS Alfardan,R., Guo,C., Toth,L.A. and Nie,D.
TITLE Impaired Recovery from Influenza A/X-31(H3N2) Infection in Mice
with 8-Lipoxygenase Deficiency
JOURNAL Med Sci (Basel) 7 (4), 60 (2019)
PUBMED 31013822
REMARK Publication Status: Online-Only
REFERENCE 6 (bases 1 to 3273)
AUTHORS Kim,E., Muga,S.J. and Fischer,S.M.
TITLE Identification and characterization of a phorbol ester-responsive
element in the murine 8S-lipoxygenase gene
JOURNAL J Biol Chem 279 (12), 11188-11197 (2004)
PUBMED 14711820
REFERENCE 7 (bases 1 to 3273)
AUTHORS Krieg,P., Kinzig,A., Heidt,M., Marks,F. and Furstenberger,G.
TITLE cDNA cloning of a 8-lipoxygenase and a novel epidermis-type
lipoxygenase from phorbol ester-treated mouse skin
JOURNAL Biochim Biophys Acta 1391 (1), 7-12 (1998)
PUBMED 9518531
REFERENCE 8 (bases 1 to 3273)
AUTHORS Jisaka,M., Kim,R.B., Boeglin,W.E., Nanney,L.B. and Brash,A.R.
TITLE Molecular cloning and functional expression of a phorbol
ester-inducible 8S-lipoxygenase from mouse skin
JOURNAL J Biol Chem 272 (39), 24410-24416 (1997)
PUBMED 9305900
REFERENCE 9 (bases 1 to 3273)
AUTHORS Furstenberger,G., Hagedorn,H., Jacobi,T., Besemfelder,E.,
Stephan,M., Lehmann,W.D. and Marks,F.
TITLE Characterization of an 8-lipoxygenase activity induced by the
phorbol ester tumor promoter 12-O-tetradecanoylphorbol-13-acetate
in mouse skin in vivo
JOURNAL J Biol Chem 266 (24), 15738-15745 (1991)
PUBMED 1874732
REFERENCE 10 (bases 1 to 3273)
AUTHORS Fischer,S.M., Baldwin,J.K., Jasheway,D.W., Patrick,K.E. and
Cameron,G.S.
TITLE Phorbol ester induction of 8-lipoxygenase in inbred SENCAR (SSIN)
but not C57BL/6J mice correlated with hyperplasia, edema, and
oxidant generation but not ornithine decarboxylase induction
JOURNAL Cancer Res 48 (3), 658-664 (1988)
PUBMED 3335028
COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from AL645527.20 and AK028724.1.
On Jan 26, 2013 this sequence version replaced NM_009661.3.
Summary: This gene belongs to the lipoxygenase (LOX) gene family
whose members encode enzymes that catalyze the addition of
molecular oxygen to polyunsaturated fatty acids (PUFAs) to yield
fatty acid hydroperoxides. The encoded enzyme preferentially
metabolizes arachidonic acid to yield 8-hydroxyeicosatetraenoic
acid (8-HETE), while metabolizing linoleic acid less efficiently.
The gene may also function as a tumor suppressor. This gene is
located in a cluster of related genes that spans approximately 75
kilobases on chromosome 11. [provided by RefSeq, Jan 2013].
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: AK028724.1, BC015253.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns
SAMN01164136 [ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
RefSeq Select criteria :: based on single protein-coding transcript
##RefSeq-Attributes-END##
COMPLETENESS: full length.
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-6 AL645527.20 114401-114406 c
7-3273 AK028724.1 3-3269
FEATURES Location/Qualifiers
source 1..3273
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6"
/db_xref="taxon:10090"
/chromosome="11"
/map="11 42.38 cM"
gene 1..3273
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/note="arachidonate 8-lipoxygenase"
/db_xref="GeneID:11688"
/db_xref="MGI:MGI:1098228"
exon 1..181
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/inference="alignment:Splign:2.1.0"
misc_feature 8
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/note="transcription initiation site (PMID 14711820); the
5' most initiation site has not been determined"
misc_feature 11..13
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/note="upstream in-frame stop codon"
CDS 35..2068
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/EC_number="1.13.11.58"
/note="arachidonate 15-lipoxygenase B; 15-lipoxygenase 2;
arachidonate 15-lipoxygenase type II; arachidonate
15-lipoxygenase, second type; 8S-lipoxygenase;
arachidonate 8S-lipoxygenase; linoleate 9S-lipoxygenase
ALOX8"
/codon_start=1
/product="polyunsaturated fatty acid lipoxygenase ALOX8"
/protein_id="NP_033791.1"
/db_xref="CCDS:CCDS24886.1"
/db_xref="GeneID:11688"
/db_xref="MGI:MGI:1098228"
/translation="MAKCRVRVSTGEACGAGTWDKVSVSIVGTHGESPLVPLDHLGKE
FSAGAEEDFEVTLPQDVGTVLMLRVHKAPPEVSLPLMSFRSDAWFCRWFELEWLPGAA
LHFPCYQWLEGAGELVLREGAAKVSWQDHHPTLQDQRQKELESRQKMYSWKTYIEGWP
RCLDHETVKDLDLNIKYSAMKNAKLFFKAHSAYTELKVKGLLDRTGLWRSLREMRRLF
NFRKTPAAEYVFAHWQEDAFFASQFLNGINPVLIRRCHSLPNNFPVTDEMVAPVLGPG
TSLQAELEKGSLFLVDHGILSGVHTNILNGKPQFSAAPMTLLHQSSGSGPLLPIAIQL
KQTPGPDNPIFLPSDDTWDWLLAKTWVRNSEFYIHEAVTHLLHAHLIPEVFALATLRQ
LPRCHPLFKLLIPHIRYTLHINTLARELLVAPGKLIDKSTGLGTGGFSDLIKRNMEQL
NYSVLCLPEDIRARGVEDIPGYYYRDDGMQIWGAIKSFVSEIVSIYYPSDTSVQDDQE
LQAWVREIFSEGFLGRESSGMPSLLDTREALVQYITMVIFTCSAKHAAVSSGQFDSCV
WMPNLPPTMQLPPPTSKGQARPESFIATLPAVNSSSYHIIALWLLSAEPGDQRPLGHY
PDEHFTEDAPRRSVAAFQRKLIQISKGIRERNRGLALPYTYLDPPLIENSVSI"
exon 182..404
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/inference="alignment:Splign:2.1.0"
exon 405..486
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/inference="alignment:Splign:2.1.0"
exon 487..609
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/inference="alignment:Splign:2.1.0"
exon 610..713
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/inference="alignment:Splign:2.1.0"
exon 714..886
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/inference="alignment:Splign:2.1.0"
exon 887..1033
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/inference="alignment:Splign:2.1.0"
exon 1034..1237
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/inference="alignment:Splign:2.1.0"
exon 1238..1324
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/inference="alignment:Splign:2.1.0"
exon 1325..1494
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/inference="alignment:Splign:2.1.0"
exon 1495..1616
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/inference="alignment:Splign:2.1.0"
exon 1617..1717
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/inference="alignment:Splign:2.1.0"
exon 1718..1888
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/inference="alignment:Splign:2.1.0"
exon 1889..3273
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/inference="alignment:Splign:2.1.0"
regulatory 2889..2894
/regulatory_class="polyA_signal_sequence"
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/note="hexamer: TATAAA"
polyA_site 2907
/gene="Alox8"
/gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
/note="major polyA site"
ORIGIN
1 cagcttgcag tagagagcta aactggtcag gaggatggcg aaatgcaggg tgagagtatc
61 cacgggggaa gcctgtgggg ctggcacatg ggacaaagtg tctgtcagca tcgtgggaac
121 ccacggagag agccccttag tacctctgga ccatctgggc aaggagttca gcgccggtgc
181 tgaagaagac ttcgaggtga cgcttcccca ggacgtaggc actgtgctga tgctgcgagt
241 ccacaaagca cccccggaag tgtccctccc gcttatgtct ttccgttctg atgcctggtt
301 ctgccgctgg ttcgagctgg agtggctacc tggggctgca ctccacttcc cctgttatca
361 gtggctggaa ggggcggggg agctggtgct gagagaggga gcagcaaagg tgtcctggca
421 agaccatcac cctacactgc aggatcagcg ccagaaggag cttgagtcca ggcagaagat
481 gtacagctgg aagacttaca ttgaaggttg gcctcgctgc cttgaccacg agactgtgaa
541 agacttggac ctcaacatca agtactctgc gatgaagaat gccaaactct tctttaaagc
601 ccactccgcg tatacggagc tgaaagtcaa agggctcctg gaccgcacag gactctggag
661 gagtctgagg gagatgagaa ggctgtttaa cttccgcaag actccagcag cagagtatgt
721 gtttgcacac tggcaggaag atgccttctt cgcctcccag ttcctaaatg gcatcaaccc
781 ggtcctgatt cgccgctgtc acagtctccc aaacaacttc ccggtcactg atgaaatggt
841 ggccccagtg ctgggccctg gaaccagtct gcaggctgag ttggagaagg gctccctgtt
901 cttggtggat catggcattc tttctggagt ccacaccaac atcctcaatg gaaagcctca
961 gttctctgca gccccgatga ccctgttaca ccagagctca gggtccggac ccctgcttcc
1021 cattgccatc cagctcaaac agactcccgg gccagacaac cccatcttcc tgcccagcga
1081 tgacacgtgg gactggttgc tggccaagac ctgggttcgc aattctgagt tttacatcca
1141 tgaggctgtc acacatctgc tgcatgccca tctgattcca gaagtctttg ccttggccac
1201 attacgtcag ctgcctaggt gtcaccctct cttcaagcta ttgattcctc acattcggta
1261 cacactgcac atcaacacgc ttgcccggga gctgctcgtt gcccctggga agttgataga
1321 caagtccaca ggccttggca ctgggggatt ctctgacctg ataaagagaa acatggagca
1381 gctgaactac tctgtcctgt gtctccctga agatatccga gcccgaggtg tggaagacat
1441 cccaggctac tattaccgag atgatgggat gcagatctgg ggggcaataa agagctttgt
1501 ctctgaaata gtcagcatct actatccaag tgacacatcc gtccaagatg accaagagct
1561 ccaggcctgg gtgagggaga tcttctctga gggcttcctc ggccgagaaa gctcaggtat
1621 gccctccttg ttggataccc gggaagccct ggtccagtat atcaccatgg tgatattcac
1681 ctgctcagcc aagcatgcag ctgtcagttc aggccagttc gactcttgtg tttggatgcc
1741 caatctgcca cctaccatgc agctaccacc acctacttcc aaaggccagg cccggcctga
1801 gagtttcata gccacgctcc cagcagttaa ttcgtcaagt tatcacatca ttgctctctg
1861 gctgctaagc gcagaacctg gggaccaaag gcccctgggc cactatccag atgaacactt
1921 cacagaggat gccccccggc gaagcgtggc tgccttccag agaaagctga tccagatctc
1981 caagggcatc agggagagga accgaggcct ggcactgccc tacacctacc tggatcctcc
2041 cctcattgag aacagtgtct ccatctaaca tcttggagaa gacagtcctg tgtgacatat
2101 agaactcttg accatgcctc tccaggctaa gtccccgtat gcttctcctg gacaaccaag
2161 ccccatctta cacacacaca cacacacaca cacctaataa aatcgaaaca gaaaaaccta
2221 aactcccaca gaaggcaaga tctcacacag cagagagcca tccaaatgtt tggagaccct
2281 gagcttcagc tctgattaac ggctttgctg gtttgctttg ctttctattc cattaaccat
2341 ggacggtaac agaaagcaca gaaccctggt tcactgcaca aagccactga gatctcaccc
2401 tcacctgaca caaaggcagc tatcatacag gcttatcagg aacacaggaa tttgtccaat
2461 caaagcctac ccactaggtc catcgtgacc tacgacctca cactggcatg ctttagcttt
2521 gagaagggat tactggagtc aggtacgaag agaaggacag gacgaaggca tggctccatg
2581 tggaagaaca tatctgctct tccagatgac cagggtagct cacagccatg tgtcattcta
2641 actccagagg tctctagtgg ccatgaagac tccaggcatt caggggatat accagtagac
2701 accaaaatta tactttttaa gagagaggaa tgggctggag agatggctca gcggttaaga
2761 gcactgactg ctcttccaga gatcctgagt tcaattccca gcaaccacat ggtggctcac
2821 aaccatctgt aatgggattc gatgccctct tctggcgtgt ctgaagacag cgacagtgta
2881 tgcacatata taaaataaat aaatctttaa aaaacaaaac aagagagagg gacatgctac
2941 catttctacc tcacttcttc tcaaagccac ccctaaagtg aattgtgaac caggtcccct
3001 ttgcagagag ttagaagata ttctcaaacc tctaatacct tcacatctaa aatccatctt
3061 cattccaaaa ttccaatatt ttatatacac tctccagttt ggtgggtgag gggttgtttt
3121 ttgtttggtt tggtttggtt ggggttttgt ttttgttttt gattttgttt ttctctggtt
3181 cagactccat ggacgttcat taatgtcata aatgagttca ttccaagttg cctggtgatt
3241 ttcatattac aggcatcaaa gttttggatc ttc
//