LOCUS NM_020873 5960 bp mRNA linear PRI 01-JUL-2024
DEFINITION Homo sapiens leucine rich repeat neuronal 1 (LRRN1), transcript
variant 3, mRNA.
ACCESSION NM_020873
VERSION NM_020873.7
KEYWORDS RefSeq; MANE Select.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5960)
AUTHORS Antikainen,A.A., Haukka,J.K., Kumar,A., Syreeni,A.,
Hagg-Holmberg,S., Ylinen,A., Kilpelainen,E., Kytola,A., Palotie,A.,
Putaala,J., Thorn,L.M., Harjutsalo,V., Groop,P.H. and Sandholm,N.
CONSRTM FinnDiane Study Group
TITLE Whole-genome sequencing identifies variants in ANK1, LRRN1, HAS1,
and other genes and regulatory regions for stroke in type 1
diabetes
JOURNAL Sci Rep 14 (1), 13453 (2024)
PUBMED 38862513
REMARK GeneRIF: Whole-genome sequencing identifies variants in ANK1,
LRRN1, HAS1, and other genes and regulatory regions for stroke in
type 1 diabetes.
Publication Status: Online-Only
REFERENCE 2 (bases 1 to 5960)
AUTHORS Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
TITLE A reference map of the human binary protein interactome
JOURNAL Nature 580 (7803), 402-408 (2020)
PUBMED 32296183
REFERENCE 3 (bases 1 to 5960)
AUTHORS Liu,B., Zhang,Y., Fan,Y., Wang,S., Li,Z., Deng,M., Li,C., Wang,J.,
Ma,R., Wang,X., Wang,Y., Xu,L., Hou,K., Che,X., Liu,Y. and Qu,X.
TITLE Leucine-rich repeat neuronal protein-1 suppresses apoptosis of
gastric cancer cells through regulation of Fas/FasL
JOURNAL Cancer Sci 110 (7), 2145-2155 (2019)
PUBMED 31087525
REMARK GeneRIF: Study found that LRRN1 is upregulated in gastric cancer
(GC) tissue samples and its expression is closely related to poor
prognosis. Knocking down LRRN1 expression promoted GC cell
apoptosis, and activated the Fas/FasL pathway.
REFERENCE 4 (bases 1 to 5960)
AUTHORS Liao,C.H., Wang,Y.H., Chang,W.W., Yang,B.C., Wu,T.J., Liu,W.L.,
Yu,A.L. and Yu,J.
TITLE Leucine-Rich Repeat Neuronal Protein 1 Regulates Differentiation of
Embryonic Stem Cells by Post-Translational Modifications of
Pluripotency Factors
JOURNAL Stem Cells 36 (10), 1514-1524 (2018)
PUBMED 29893054
REMARK GeneRIF: Authors found that LRRN1 contributes to pluripotency of
hESC by preventing translocation of OCT4, NANOG, and SOX2 from
nucleus to cytoplasm, thereby lessening their post-translational
modification and degradation.
REFERENCE 5 (bases 1 to 5960)
AUTHORS Satoh,S., Takatori,A., Ogura,A., Kohashi,K., Souzaki,R.,
Kinoshita,Y., Taguchi,T., Hossain,M.S., Ohira,M., Nakamura,Y. and
Nakagawara,A.
TITLE Neuronal leucine-rich repeat 1 negatively regulates anaplastic
lymphoma kinase in neuroblastoma
JOURNAL Sci Rep 6, 32682 (2016)
PUBMED 27604320
REMARK GeneRIF: NLRR1 appears to be an extracellular negative regulator of
ALK signalling in neuroblastoma and neuronal development.
Publication Status: Online-Only
REFERENCE 6 (bases 1 to 5960)
AUTHORS Sousa,I., Clark,T.G., Holt,R., Pagnamenta,A.T., Mulder,E.J.,
Minderaa,R.B., Bailey,A.J., Battaglia,A., Klauck,S.M., Poustka,F.
and Monaco,A.P.
CONSRTM International Molecular Genetic Study of Autism Consortium (IMGSAC)
TITLE Polymorphisms in leucine-rich repeat genes are associated with
autism spectrum disorder susceptibility in populations of European
ancestry
JOURNAL Mol Autism 1 (1), 7 (2010)
PUBMED 20678249
REMARK GeneRIF: Observational study of gene-disease association. (HuGE
Navigator)
Publication Status: Online-Only
REFERENCE 7 (bases 1 to 5960)
AUTHORS Hossain,M.S., Ozaki,T., Wang,H., Nakagawa,A., Takenobu,H.,
Ohira,M., Kamijo,T. and Nakagawara,A.
TITLE N-MYC promotes cell proliferation through a direct transactivation
of neuronal leucine-rich repeat protein-1 (NLRR1) gene in
neuroblastoma
JOURNAL Oncogene 27 (46), 6075-6082 (2008)
PUBMED 18591937
REMARK GeneRIF: N-MYC promotes cell proliferation through a direct
transactivation of neuronal leucine-rich repeat protein-1 (NLRR1)
gene in neuroblastoma.
REFERENCE 8 (bases 1 to 5960)
AUTHORS Zhang,Z. and Henzel,W.J.
TITLE Signal peptide prediction based on analysis of experimentally
verified cleavage sites
JOURNAL Protein Sci 13 (10), 2819-2824 (2004)
PUBMED 15340161
REFERENCE 9 (bases 1 to 5960)
AUTHORS Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
TITLE The secreted protein discovery initiative (SPDI), a large-scale
effort to identify novel human secreted and transmembrane proteins:
a bioinformatics assessment
JOURNAL Genome Res 13 (10), 2265-2270 (2003)
PUBMED 12975309
REMARK Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE 10 (bases 1 to 5960)
AUTHORS Ohira,M., Morohashi,A., Nakamura,Y., Isogai,E., Furuya,K.,
Hamano,S., Machida,T., Aoyama,M., Fukumura,M., Miyazaki,K.,
Suzuki,Y., Sugano,S., Hirato,J. and Nakagawara,A.
TITLE Neuroblastoma oligo-capping cDNA project: toward the understanding
of the genesis and biology of neuroblastoma
JOURNAL Cancer Lett 197 (1-2), 63-68 (2003)
PUBMED 12880961
REMARK Review article
COMMENT VALIDATED REFSEQ: This record has undergone validation or
preliminary review. The reference sequence was derived from
DA736067.1, AC023484.5, AK291717.1, BC034947.2, BU685265.1,
AU154359.1, CX867219.1 and BC012463.1.
On May 17, 2019 this sequence version replaced NM_020873.6.
Transcript Variant: Variants 1, 2 and 3 encode the same protein.
Sequence Note: This RefSeq record was created from transcript and
genomic sequence data to make the sequence consistent with the
reference genome assembly. The genomic coordinates used for the
transcript record were based on transcript alignments.
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: SRR1660805.151344.1,
SRR1660809.78102.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns
SAMEA1968189, SAMEA1968968
[ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
MANE Ensembl match :: ENST00000319331.4/ ENSP00000314901.3
RefSeq Select criteria :: based on conservation, expression,
longest protein
##RefSeq-Attributes-END##
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-105 DA736067.1 1-105
106-106 AC023484.5 129349-129349
107-316 DA736067.1 107-316
317-317 AC023484.5 129560-129560
318-416 DA736067.1 318-416
417-621 AK291717.1 1-205
622-2512 AC023484.5 174309-176199
2513-3112 AK291717.1 2097-2696
3113-3521 BC034947.2 2676-3084
3522-3523 AC023484.5 177209-177210
3524-3663 BC034947.2 3085-3224
3664-3664 AC023484.5 177351-177351
3665-3743 BC034947.2 3226-3304
3744-4150 BU685265.1 17-423 c
4151-4340 AU154359.1 3-192 c
4341-4675 AC023484.5 178028-178362
4676-5353 CX867219.1 160-837
5354-5603 BC012463.1 292-541
5604-5872 AC023484.5 179291-179559
5873-5960 BC012463.1 811-898
FEATURES Location/Qualifiers
source 1..5960
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="3"
/map="3p26.2"
gene 1..5960
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="leucine rich repeat neuronal 1"
/db_xref="GeneID:57633"
/db_xref="HGNC:HGNC:20980"
/db_xref="MIM:619623"
exon 1..489
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/inference="alignment:Splign:2.1.0"
exon 490..5960
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/inference="alignment:Splign:2.1.0"
misc_feature 723..725
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="upstream in-frame stop codon"
CDS 768..2918
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="fibronectin type III, immunoglobulin and leucine
rich repeat domains 3; leucine-rich repeat neuronal
protein 1; neuronal leucine-rich repeat protein 1"
/codon_start=1
/product="leucine-rich repeat neuronal protein 1
precursor"
/protein_id="NP_065924.3"
/db_xref="CCDS:CCDS33685.1"
/db_xref="GeneID:57633"
/db_xref="HGNC:HGNC:20980"
/db_xref="MIM:619623"
/translation="MARMSFVIAACQLVLGLLMTSLTESSIQNSECPQLCVCEIRPWF
TPQSTYREATTVDCNDLRLTRIPSNLSSDTQVLLLQSNNIAKTVDELQQLFNLTELDF
SQNNFTNIKEVGLANLTQLTTLHLEENQITEMTDYCLQDLSNLQELYINHNQISTISA
HAFAGLKNLLRLHLNSNKLKVIDSRWFDSTPNLEILMIGENPVIGILDMNFKPLANLR
SLVLAGMYLTDIPGNALVGLDSLESLSFYDNKLVKVPQLALQKVPNLKFLDLNKNPIH
KIQEGDFKNMLRLKELGINNMGELVSVDRYALDNLPELTKLEATNNPKLSYIHRLAFR
SVPALESLMLNNNALNAIYQKTVESLPNLREISIHSNPLRCDCVIHWINSNKTNIRFM
EPLSMFCAMPPEYKGHQVKEVLIQDSSEQCLPMISHDSFPNRLNVDIGTTVFLDCRAM
AEPEPEIYWVTPIGNKITVETLSDKYKLSSEGTLEISNIQIEDSGRYTCVAQNVQGAD
TRVATIKVNGTLLDGTQVLKIYVKQTESHSILVSWKVNSNVMTSNLKWSSATMKIDNP
HITYTARVPVDVHEYNLTHLQPSTDYEVCLTVSNIHQQTQKSCVNVTTKNAAFAVDIS
DQETSTALAAVMGSMFAVISLASIAVYFAKRFKRKNYHHSLKKYMQKTSSIPLNELYP
PLINLWEGDSEKDKDGSADTKPTQVDTSRSYYMW"
sig_peptide 768..842
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/inference="COORDINATES: ab initio prediction:SignalP:6.0"
mat_peptide 843..2915
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/product="Leucine-rich repeat neuronal protein 1.
/id=PRO_0000014847"
/note="propagated from UniProtKB/Swiss-Prot (Q6UXK5.1)"
misc_feature 984..1052
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="propagated from UniProtKB/Swiss-Prot (Q6UXK5.1);
Region: LRR 1"
misc_feature 1053..1118
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="propagated from UniProtKB/Swiss-Prot (Q6UXK5.1);
Region: LRR 2"
misc_feature 1053..1055
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000255; propagated from
UniProtKB/Swiss-Prot (Q6UXK5.1); glycosylation site"
misc_feature 1116..1118
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000255; propagated from
UniProtKB/Swiss-Prot (Q6UXK5.1); glycosylation site"
misc_feature 1125..1190
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="propagated from UniProtKB/Swiss-Prot (Q6UXK5.1);
Region: LRR 3"
misc_feature 1197..1262
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="propagated from UniProtKB/Swiss-Prot (Q6UXK5.1);
Region: LRR 4"
misc_feature 1269..1334
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="propagated from UniProtKB/Swiss-Prot (Q6UXK5.1);
Region: LRR 5"
misc_feature 1341..1406
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="propagated from UniProtKB/Swiss-Prot (Q6UXK5.1);
Region: LRR 6"
misc_feature 1413..1478
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="propagated from UniProtKB/Swiss-Prot (Q6UXK5.1);
Region: LRR 7"
misc_feature 1485..1550
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="propagated from UniProtKB/Swiss-Prot (Q6UXK5.1);
Region: LRR 8"
misc_feature 1557..1622
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="propagated from UniProtKB/Swiss-Prot (Q6UXK5.1);
Region: LRR 9"
misc_feature 1704..1772
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="propagated from UniProtKB/Swiss-Prot (Q6UXK5.1);
Region: LRR 10"
misc_feature 1779..1844
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="propagated from UniProtKB/Swiss-Prot (Q6UXK5.1);
Region: LRR 11"
misc_feature 1920..1922
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000255; propagated from
UniProtKB/Swiss-Prot (Q6UXK5.1); glycosylation site"
misc_feature 2316..2318
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000255; propagated from
UniProtKB/Swiss-Prot (Q6UXK5.1); glycosylation site"
misc_feature 2661..2723
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="propagated from UniProtKB/Swiss-Prot (Q6UXK5.1);
transmembrane region"
misc_feature 2838..2915
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="propagated from UniProtKB/Swiss-Prot (Q6UXK5.1);
Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
regulatory 3716..3721
/regulatory_class="polyA_signal_sequence"
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="hexamer: AATAAA"
polyA_site 3746
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="major polyA site"
regulatory 3808..3813
/regulatory_class="polyA_signal_sequence"
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="hexamer: CATAAA"
polyA_site 3831
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="major polyA site"
regulatory 4322..4327
/regulatory_class="polyA_signal_sequence"
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
/note="hexamer: AGTAAA"
polyA_site 4346
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
polyA_site 5960
/gene="LRRN1"
/gene_synonym="FIGLER3; NLRR-1; NLRR1"
ORIGIN
1 gagcacaaag cggggcgcac cgcgggcgcc ggcaacgagc cggtgaacga ggcgaggccc
61 gtgcgcccgc ggctgcaagc gcccgcctgg cggggagagg ggccgacggc gtcagcccgg
121 gcggcggcat ccctaggcgc ctggggcgcc ttcctccgga cctggccgct cgctgccccg
181 ccctctgcac cccacttctc cgaccctcct tcccagtcct gcctccccct gccctggcct
241 ctgagagccg actgagccca gccccgtgca gcagcggttg cctgtgtcgc cgcctagtct
301 ccggtcttgg tgctctcccg ggggtgcccc aaggagccag tgcgcgctgc gggctgggaa
361 ggaggcgccg ctcagctagt cctcctcctc ctcctcgtct ttctcctcct cctgctgctg
421 ctgccgccgc cgccgccgtg ggtgccgggt ccgcgcgcac cccaacaccc ccaccagctg
481 ggcctcgggg cacatctcca gacttcaatt tggctgaaat aattcatgcc acggacctgt
541 gcacatgcct ggaattgaga gacacagtta aaagactcca agttgctttc tgccttttga
601 aaactcctga aaaccatccc tttggactct ggaattctac acagctcaac caagactttg
661 cttgaatgtt tacattttct gctcgctgtc ctacatatca caatatagtg ttcacgtttt
721 gttaaaactt tggggtgtca ggagttgagc ttgctcagca agccagcatg gctaggatga
781 gctttgttat agcagcttgc caattggtgc tgggcctact aatgacttca ttaaccgagt
841 cttccataca gaatagtgag tgtccacaac tttgcgtatg tgaaattcgt ccctggttta
901 ccccacagtc aacttacaga gaagccacca ctgttgattg caatgacctc cgcttaacaa
961 ggattcccag taacctctct agtgacacac aagtgcttct cttacagagc aataacatcg
1021 caaagactgt ggatgagctg cagcagcttt tcaacttgac tgaactagat ttctcccaaa
1081 acaactttac taacattaag gaggtcgggc tggcaaacct aacccagctc acaacgctgc
1141 atttggagga aaatcagatt accgagatga ctgattactg tctacaagac ctcagcaacc
1201 ttcaagaact ctacatcaac cacaaccaaa ttagcactat ttctgctcat gcttttgcag
1261 gcttaaaaaa tctattaagg ctccacctga actccaacaa attgaaagtt attgatagtc
1321 gctggtttga ttctacaccc aacctggaaa ttctcatgat cggagaaaac cctgtgattg
1381 gaattctgga tatgaacttc aaacccctcg caaatttgag aagcttagtt ttggcaggaa
1441 tgtatctcac tgatattcct ggaaatgctt tggtgggtct ggatagcctt gagagcctgt
1501 ctttttatga taacaaactg gttaaagtcc ctcaacttgc cctgcaaaaa gttccaaatt
1561 tgaaattctt agacctcaac aaaaacccca ttcacaaaat ccaagaaggg gacttcaaaa
1621 atatgcttcg gttaaaagaa ctgggaatca acaatatggg cgagctcgtt tctgtcgacc
1681 gctatgccct ggataacttg cctgaactca caaagctgga agccaccaat aaccctaaac
1741 tctcttacat ccaccgcttg gctttccgaa gtgtccctgc tctggaaagc ttgatgctga
1801 acaacaatgc cttgaatgcc atttaccaaa agacagtcga atccctcccc aatctgcgtg
1861 agatcagtat ccatagcaat cccctcaggt gtgactgtgt gatccactgg attaactcca
1921 acaaaaccaa catccgcttc atggagcccc tgtccatgtt ctgtgccatg ccgcccgaat
1981 ataaagggca ccaggtgaag gaagttttaa tccaggattc gagtgaacag tgcctcccaa
2041 tgatatctca cgacagcttc ccaaatcgtt taaacgtgga tatcggcacg acggttttcc
2101 tagactgtcg agccatggct gagccagaac ctgaaattta ctgggtcact cccattggaa
2161 ataagataac tgtggaaacc ctttcagata aatacaagct aagtagcgaa ggtaccttgg
2221 aaatatctaa catacaaatt gaagactcag gaagatacac atgtgttgcc cagaatgtcc
2281 aaggggcaga cactcgggtg gcaacaatta aggttaatgg gacccttctg gatggtaccc
2341 aggtgctaaa aatatacgtc aagcagacag aatcccattc catcttagtg tcctggaaag
2401 ttaattccaa tgtcatgacg tcaaacttaa aatggtcgtc tgccaccatg aagattgata
2461 accctcacat aacatatact gccagggtcc cagtcgatgt ccatgaatac aacctaacgc
2521 atctgcagcc ttccacagat tatgaagtgt gtctcacagt gtccaatatt catcagcaga
2581 ctcaaaagtc atgcgtaaat gtcacaacca aaaatgccgc cttcgcagtg gacatctctg
2641 atcaagaaac cagtacagcc cttgctgcag taatggggtc tatgtttgcc gtcattagcc
2701 ttgcgtccat tgctgtgtac tttgccaaaa gatttaagag aaaaaactac caccactcat
2761 taaaaaagta tatgcaaaaa acctcttcaa tcccactaaa tgagctgtac ccaccactca
2821 ttaacctctg ggaaggtgac agcgagaaag acaaagatgg ttctgcagac accaagccaa
2881 cccaggtcga cacatccaga agctattaca tgtggtaact cagaggatat tttgcttctg
2941 gtagtaagga gcacaaagac gtttttgctt tattctgcaa aagtgaacaa gttgaagact
3001 tttgtatttt tgactttgct agtttgtggc agagtggaga ggacgggtgg atatttcaaa
3061 tttttttagt atagcgtatc gcaagggttt gacacggctg ccagcgactc taggcttcca
3121 gtctgtgttt ggtttttatt cttatcatta ttatgattgt tattatatta ttattttatt
3181 ttagttgttg tgctaaactc aataatgctg ttctaactac agtgctcaat aaaatgatta
3241 atgacaggat ggggttcccc tgtgctttta ccagtagcat gaccccttct gaagccatcc
3301 gtagaaagta ctttgtcctc caaaaagcta acatacggtt ttgaagcagc attgaaactt
3361 ttgtagcaat ctggtctata gacttttaac tcaagaagct aaggctagac ttgttacctt
3421 cgttgaatga tgttagttga ctgtactgta atgttgtatc aactgaattg aatgtttgcc
3481 tttaaacaat gaattttctt tttctttcct tttttttttt tttgttgtaa tagttaaaga
3541 ggcttagaac aagctaacag gcaatagaaa tatgtatatc agatttttta atgtaacaaa
3601 ctacatgtta attgttatct tattcttttt atctttagta gacactttta aaagaaaaga
3661 caagtttgtt gtgtttaact caccaacacg tggtgtataa tgaagacaga actataataa
3721 attagttttg ttctgatttt ttagaacact tgcaataatg tatcatttat agttcttgct
3781 agttgcagtg gtaatatttt tcacatccat aaaaacaact accaaaataa atcagctgta
3841 gcatgttgct ttttaaagct aggccctaaa aggttttaat tctttttcta agggaagaaa
3901 tgtctatttt aattaagata ttttaatgaa caggatttct gtattttaaa tagtactgac
3961 tagcacctaa tgggcagtgg gagggtggtt catatgaaga aaaaaaggtg tattgttgta
4021 tcccatgcat aaataaaggt aaatatatat atacaccaat atattcatat atactcacac
4081 acatcccaac ctgtcacaca caatgcgtgt gtatatatat atgaatatat tggatatgtc
4141 atttctgtaa gagttttgtt aaaacctgat tttcttttgt agtatccaca ttcttcatca
4201 aagtacaaaa acgtctgtgg agtgtcacaa actgtatgac atgttatttc tttttaacag
4261 ttgtctatat gcttagaccc gtgttagtct ctatatctgt gtggcaatat ctgctgagac
4321 aagtaaatga ttaatagaaa acaaaacaac ttccgtacag ttcaaacttt tcatccaaat
4381 atatatagac agttttggag aattgtttca agattataga ggaaacatgt aacatttaga
4441 gcagatggaa ctaggtttag gtagaaggcc agttccacaa agggcagagg gagggatggg
4501 atttaatagg taaagaagaa cccatttgaa aataaaggtt gtttcaaaag gcagctgccg
4561 ccaggcacac agcattccat cagacaggtg ccagacaagc aaaagcaagg aagttggcag
4621 aaagaaagtc caggtgatgt aggttgaggt atttctttct ttggcagtat cttgcttttg
4681 tgaatcactt tattaaaaaa aatcactttc ttcccattct taaggggttt tggcaaacag
4741 aatttcagat cttgaaacaa atgagctgca acagaaaaat aagtacctga gctcgaggta
4801 tcccttccca aagccttcac ttattggtga gaaaaacctg ggcccaggga gggcttgcag
4861 cttacccagt tcggactcct gccagttcag cgctctgcac tccattgatt gttctaggcc
4921 cggcagcctg ttaggttcct gtgcagggcc cctcttttgc agttctgaat aacctctagt
4981 gcccctgctt caaaaatgca gtacatcctc aagttcccat ttactcagga tacattttag
5041 cacagggcag atcagacggt gtctctactg ttagtattgc aaagtatgta tgggaaacac
5101 agagaattgg agctgcgttg aatgcaaact tgaggtgttt cccttgagga attcttgtct
5161 tcaaacgtct gcagagtaat ggaccatgtt acaactttcc tgttcatctg tgaaccatga
5221 aaatggatgg cactgatgca ttagaccctc agcagcctgc aattgcaaat ctgcgaggtt
5281 tcattcggcc cataaagcaa acatttgaac ttacacagaa tgagcactta aatacgggtg
5341 caataaatga agggaaaaac ctcagccgtt tctccattct gaagatatag caagcaccgg
5401 gaaatctaag atttttcatc aacaatatct tctgccagcc cagtttgggg ggaaaaaccc
5461 cttttacatt tttcttcagt aaagtactgg aacttacttt tccctgtctg tgctaatgag
5521 ctgattttca gctgatagaa aacaaaatga tagagtactt ttttccttgg ccaagtattt
5581 tctcatttgt atttaatttc ataaattaga cagccagtga aattagacct caaactaggt
5641 cctgatggat aatgaatgtt atgtcacctt taacagtgaa gtggttatta taggtcactt
5701 tctaatttca tattttccct tttgctttct gctgccttca gggtatatag tgtatctcta
5761 acctgatttt tcaaggttat ttttggagca gtttcttaaa acaggcattc cctaacttgc
5821 tcatttaatt aatgaaaaat tgaactgatg ccatggatat aaaaacaaat gtaatgtttg
5881 attgtcagtg tttctgattt ggcaaaaagg aatcatctct atttttttgc aaacaatatc
5941 aaagtgcata ttttctctca
//