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Homo sapiens protein phosphatase 4 regulatory subunit 4 (PPP4R4), transcript variant 1, mRNA

NCBI Reference Sequence: NM_058237.2

FASTA Graphics 

LOCUS       NM_058237               3857 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens protein phosphatase 4 regulatory subunit 4 (PPP4R4),
            transcript variant 1, mRNA.
ACCESSION   NM_058237
VERSION     NM_058237.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   3  (bases 1 to 3857)
  AUTHORS   St-Denis,N., Gupta,G.D., Lin,Z.Y., Gonzalez-Badillo,B., Veri,A.O.,
            Knight,J.D.R., Rajendran,D., Couzens,A.L., Currie,K.W., Tkach,J.M.,
            Cheung,S.W.T., Pelletier,L. and Gingras,A.C.
  TITLE     Phenotypic and Interaction Profiling of the Human Phosphatases
            Identifies Diverse Mitotic Regulators
  JOURNAL   Cell Rep 17 (9), 2488-2501 (2016)
   PUBMED   27880917
REFERENCE   4  (bases 1 to 3857)
  AUTHORS   Chen,G.I., Tisayakorn,S., Jorgensen,C., D'Ambrosio,L.M.,
            Goudreault,M. and Gingras,A.C.
  TITLE     PP4R4/KIAA1622 forms a novel stable cytosolic complex with
            phosphoprotein phosphatase 4
  JOURNAL   J Biol Chem 283 (43), 29273-29284 (2008)
   PUBMED   18715871
  REMARK    GeneRIF: PP4R4 forms a novel cytosolic complex with PP4c,
            independent from the complexes containing PP4R1, PP4R2.PP4R3, and
            alpha4, and the regulatory subunits of PP4c have evolved different
            modes of interaction with the catalytic subunit
REFERENCE   5  (bases 1 to 3857)
  AUTHORS   Namciu,S.J., Friedman,R.D., Marsden,M.D., Sarausad,L.M.,
            Jasoni,C.L. and Fournier,R.E.
  TITLE     Sequence organization and matrix attachment regions of the human
            serine protease inhibitor gene cluster at 14q32.1
  JOURNAL   Mamm Genome 15 (3), 162-178 (2004)
   PUBMED   15014966
REFERENCE   6  (bases 1 to 3857)
  AUTHORS   Andrade,M.A., Petosa,C., O'Donoghue,S.I., Muller,C.W. and Bork,P.
  TITLE     Comparison of ARM and HEAT protein repeats
  JOURNAL   J Mol Biol 309 (1), 1-18 (2001)
   PUBMED   11491282
  REMARK    Review article
REFERENCE   7  (bases 1 to 3857)
  AUTHORS   Groves,M.R., Hanlon,N., Turowski,P., Hemmings,B.A. and Barford,D.
  TITLE     The structure of the protein phosphatase 2A PR65/A subunit reveals
            the conformation of its 15 tandemly repeated HEAT motifs
  JOURNAL   Cell 96 (1), 99-110 (1999)
   PUBMED   9989501
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB480905.1, BC068491.1 and
            AB046842.1.
            
            On Nov 23, 2018 this sequence version replaced NM_058237.1.
            
            Summary: The protein encoded by this gene is a HEAT-like
            repeat-containing protein. The HEAT repeat is a tandemly repeated,
            37-47 amino acid long module occurring in a number of cytoplasmic
            proteins. Arrays of HEAT repeats form a rod-like helical structure
            and appear to function as protein-protein interaction surfaces. The
            repeat-containing region of this protein has some similarity to the
            constant regulatory domain of the protein phosphatase 2A PR65/A
            subunit. The encoded protein binds protein serine/threonine
            phosphatase 4c in the cytoplasm. [provided by RefSeq, Jan 2017].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.137306.1,
                                           SRR1660803.23201.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2145245
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000304338.8/ ENSP00000305924.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                DB480905.1         11-41
            32-2879             BC068491.1         1-2848
            2880-3137           AB046842.1         2809-3066
            3138-3411           BC068491.1         3106-3379
            3412-3857           AB046842.1         3340-3785
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.12-q32.13"
     gene            1..3857
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /note="protein phosphatase 4 regulatory subunit 4"
                     /db_xref="GeneID:57718"
                     /db_xref="HGNC:HGNC:23788"
                     /db_xref="MIM:616790"
     exon            1..261
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    85..87
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /note="upstream in-frame stop codon"
     CDS             145..2766
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     HEAT-like repeat-containing protein;
                     serine/threonine-protein phosphatase 4 regulatory subunit
                     4; cilia and flagella associated protein 14"
                     /codon_start=1
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 4 isoform 1"
                     /protein_id="NP_478144.1"
                     /db_xref="CCDS:CCDS9921.1"
                     /db_xref="GeneID:57718"
                     /db_xref="HGNC:HGNC:23788"
                     /db_xref="MIM:616790"
                     /translation="MHPPPPAAAMDFSQNSLFGYMEDLQELTIIERPVRRSLKTPEEI
                     ERLTVDEDLSDIERAVYLLSAGQDVQGTSVIANLPFLMRQNPTETLRRVLPKVREALH
                     VAGVEMQLTAAMSFLTILQDESVSIHAYTHSFLQVILLHLEHRDTGVSNAWLETLLSV
                     IEVLPKETLRHEILNPLVSKAQLSQTVQSRLVSCKILGKLTNKFDAHTIKREILPLVK
                     SLCQDVEYEVRSCMCRQLENIAQGIGTELTKSVVLPELIELSRDEGSSVRLAAFETLV
                     NLLDIFDTDDRSQTILPLVKSFCEKSFKADESILISLSFHLGKLCHGLYGIFTPDQHL
                     RFLEFYKKLCTLGLQQENGHNENQIPPQILEQEKKYISVRKNCAYNFPAMIVFVDPKN
                     FHMELYSTFFCLCHDPEVPVRYTIAICFYEVSKLLNSGVYLIHKELITLLQDESLEVL
                     DALIDHLPEILELMSTGGESSVQENKLSSLPDLIPALTAAEQRAAASLKWRTHEKLLQ
                     KYACLPHVISSDQIYYRFLQRMFTIMMTNNVLPVQKAASRTLCIFLRYNRKQEQRHEV
                     IQKLIEQLGQGKSYWNRLRFLDTCEFIIEIFSKSFFCKYFFLPAIELTHDPVANVRMK
                     LCYLLPKVKSTLKIPADKHLLQQLEMCVRKLLCQEKDKDVLAIVKRTVLELDRMEMSM
                     DAFQKKFYEKDLLDQEKEREELLLLEMEQLEKEKQQNDGRPMSDKMFEKKRRDTKTPT
                     QSLPKNIPISVPGPSSVTPSTSKEIKKSKLIRSQSFNNQAFHAKYGNLEKCASKSSTT
                     GYTTSVSGLGKTSVLSLADDSFRTRNASSVPSSFSPNTPLPSTSRGTGNSVDPKSSGS
                     KDTQPRKATLKSRKSNP"
     misc_feature    757..873
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /note="Region: HEAT-like repeat region"
     misc_feature    781..897
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NUP7.1);
                     Region: HEAT 1"
     misc_feature    874..990
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /note="Region: HEAT-like repeat region"
     misc_feature    898..1014
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NUP7.1);
                     Region: HEAT 2"
     misc_feature    1318..1425
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NUP7.1);
                     Region: HEAT 3"
     misc_feature    2281..2442
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NUP7.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    2467..2469
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8C0Y0; propagated from
                     UniProtKB/Swiss-Prot (Q6NUP7.1); phosphorylation site"
     misc_feature    2533..2535
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q8C0Y0; propagated from
                     UniProtKB/Swiss-Prot (Q6NUP7.1); phosphorylation site"
     misc_feature    2608..2763
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NUP7.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     exon            262..335
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            336..438
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            439..586
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            587..660
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            661..767
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            768..875
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            876..997
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            998..1120
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            1121..1290
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..1410
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1488
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            1489..1572
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1755
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1861
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            1862..2009
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            2010..2154
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            2155..2196
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            2197..2271
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            2272..2341
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            2342..2428
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            2429..2522
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            2523..2593
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            2594..2741
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            2742..3857
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3836..3841
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /note="hexamer: AATAAA"
     polyA_site      3857
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /note="major polyA site"
ORIGIN      
        1 cttgctcccg ccgcctggca gcctcacgct cggctccagc ggccaagagc cggagaaagt
       61 cctgctggtg ggcggccgcg gggctgaggg cgtccggcat cccggggccg ctccggcccg
      121 ggcggcgaga gtgcccggcg gtccatgcat ccgccgccgc ccgccgccgc gatggatttc
      181 agtcagaaca gcctgttcgg ttacatggag gacctgcagg agctcaccat catcgagagg
      241 ccggtccgcc ggagcctcaa gacaccggaa gaaatagaaa gattgacagt cgatgaagac
      301 ctcagtgata ttgaaagggc tgtttatctg ctcagtgctg gtcaagatgt ccaaggaaca
      361 agtgtgattg caaatctccc atttttgatg cgacagaatc ccactgagac gcttcggaga
      421 gtgttgccaa aagtcagaga agccctgcat gttgcaggag tggaaatgca gttaacggct
      481 gcgatgtcat ttctgaccat tctgcaggac gaatcagtgt caattcatgc atatacccac
      541 tcattcctcc aagtcattct cctgcatctg gagcacaggg acacaggtgt cagcaatgca
      601 tggctggaaa ctcttctgtc tgttatagaa gtattgccaa aagaaaccct acggcatgag
      661 attttgaatc cacttgtttc caaggcacaa ctttcccaaa cagtccagtc tcgtttagtt
      721 agttgtaaaa ttttaggaaa attgaccaac aaatttgatg cccacaccat taagcgagaa
      781 atacttcctc tggtaaaatc actctgtcaa gatgtagaat atgaagttcg atcttgtatg
      841 tgtcggcaat tagaaaatat agcccagggc attgggacag aacttacaaa aagtgtggtg
      901 ctccctgaat taatagaact ttctagggat gaaggcagca gtgtacgact tgcagctttt
      961 gaaactttgg ttaatctgct tgatatattt gatacagatg acagaagtca aactatactt
     1021 cccttagtga aatcattttg tgaaaaatct ttcaaagcag atgaatcaat tcttatttct
     1081 ttatctttcc atttaggaaa actatgtcat ggactatatg gaattttcac tccagatcag
     1141 cacttgagat ttttggaatt ttataagaaa ctttgtacat tgggtttgca acaagaaaat
     1201 ggacacaatg aaaaccagat tccaccccaa atcctagagc aggagaagaa atatatttca
     1261 gtacggaaga actgtgctta taactttccg gccatgattg tttttgttga tcctaaaaac
     1321 ttccacatgg aactctattc tacattcttc tgcctttgcc atgaccctga agtaccagtc
     1381 agatacacta ttgctatttg cttttatgaa gtatctaagc ttctgaattc tggagtatat
     1441 ttaatacata aagaactaat aacattatta caagatgaat cactggaggt actagatgct
     1501 cttatagatc atcttccaga aatcttggaa cttatgtcta ctggtggaga aagcagtgtt
     1561 caagaaaata agttatcttc tctgcctgac ttgattccag cactcacagc tgctgaacag
     1621 cgagctgcag cctctttaaa atggagaact catgagaagc tacttcagaa atatgcctgc
     1681 ctgccacatg tcatatcaag cgatcagatt tattaccgtt tcttacaaag aatgttcaca
     1741 atcatgatga caaataatgt tttacctgtc caaaaggcgg cttcacgaac tctatgcatt
     1801 tttctgcgtt ataatcgtaa acaagaacag agacatgagg tcattcaaaa attaattgaa
     1861 caattgggcc aaggaaaaag ttactggaat agacttcgat ttttggatac ctgtgaattt
     1921 attatagaga tattttcaaa atcatttttc tgtaaatatt tctttctacc tgctattgaa
     1981 ctgacacatg atccagtagc aaatgtgaga atgaaacttt gctacctgtt gcccaaagtg
     2041 aaatctactc tgaagattcc tgctgataag catctacttc agcagttaga aatgtgtgtg
     2101 aggaaactcc tgtgtcaaga aaaagataaa gatgttctgg ctattgtaaa aagaactgta
     2161 ttagagttgg acagaatgga aatgtctatg gatgcttttc agaaaaagtt ttatgagaaa
     2221 gatttgttgg atcaagagaa agaaagagaa gaactacttc ttttggaaat ggaacaatta
     2281 gagaaagaaa agcaacagaa tgatggaagg cccatgagtg ataaaatgtt tgaaaagaaa
     2341 cgtagagaca ctaagacacc aacgcaaagt ctgcccaaga acatccccat ttctgttcct
     2401 ggaccctctt ctgtcacccc atcgacaagt aaagaaatca agaaatccaa actgattcga
     2461 agccagtctt ttaataatca agcttttcat gcaaaatatg gcaacttaga gaaatgtgct
     2521 agtaaaagtt ctacaacagg atatacaact tctgtctcag ggttaggaaa gacttctgtg
     2581 ctttcactag ctgatgattc attccggact cgtaatgcca gtagcgttcc atcttccttt
     2641 tctcctaata ctcccttacc gagtacttcc cgtgggacag gtaactcagt tgaccccaag
     2701 agcagtggaa gtaaagatac acaaccacgg aaggctacct taaaatccag aaaatccaat
     2761 ccttaaatca actgcttgat gaaggaggca aaacaaaggc agcaggagat aatgtgattg
     2821 gtacacaaaa gctaaagcag tggctggggc tttgttttta aattttgggt tttttttttt
     2881 gttgttgtta atgagcagaa agagagacat aatgacagct gatgttaaac ttttcatatt
     2941 tcaaattaga ttccctagga ggtataatat atatttcttg agtaataatg tggttacgga
     3001 attccaatgt tatagtgaag tgtaatgaaa aacatctcta ggaatgtgct ttaaccactg
     3061 ctgcaaaaga gacaagtctg catttatttg tgcaggaaac cagccattta attgttctag
     3121 agttttagca tttaaaaatc gtatgaaagt ctacatcagc tgaattgtcc tagcttgata
     3181 agcacttgga gggggacttg gaaggtgaga aagatactgc attttctcat gagtctccaa
     3241 gcccacttga aaagtcacac tgaaaggatg caaatagccg tctgcgattt tggcggccct
     3301 ccggattgtg gtgacaatat gttgtacccg gacattccca aatttaaatt tggcagtgaa
     3361 aattccacaa agatttctgg taactttgag ctataaatac cttaaaaata atagattgat
     3421 gattttcttt atttcctaga gaatttattt tataaatact ttgtttacat tttagattgt
     3481 acttgtcttt atttaaaatg atgaatctag tctgaatcag ctgttattcc aagctatcat
     3541 gcttaagcta tgtcaacagc atttatttgt actaatgcta atatttccat caaaatttgc
     3601 ctgtggaata tatacagttc ttaattttaa actgtcagtt cttgagatat accgtgtgaa
     3661 gctattgtca gcttctctat ttcaaaatgc aatcagcata taactatatt gatattagaa
     3721 gatatttgtt ttttaaaata tattgatgta tgataaagat gatctaattt gtgaatgcat
     3781 atgtatgtgt ggttactttt tataatgtga aataatgaat aatgaattta ctcaaaataa
     3841 aacataggtt aatgaga
//
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