LOCUS NM_058237 3857 bp mRNA linear PRI 19-SEP-2024
DEFINITION Homo sapiens protein phosphatase 4 regulatory subunit 4 (PPP4R4),
transcript variant 1, mRNA.
ACCESSION NM_058237
VERSION NM_058237.2
KEYWORDS RefSeq; MANE Select.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3857)
AUTHORS Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
Harper,J.W. and Gygi,S.P.
TITLE Dual proteome-scale networks reveal cell-specific remodeling of the
human interactome
JOURNAL Cell 184 (11), 3022-3040 (2021)
PUBMED 33961781
REFERENCE 2 (bases 1 to 3857)
AUTHORS Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
TITLE Architecture of the human interactome defines protein communities
and disease networks
JOURNAL Nature 545 (7655), 505-509 (2017)
PUBMED 28514442
REFERENCE 3 (bases 1 to 3857)
AUTHORS St-Denis,N., Gupta,G.D., Lin,Z.Y., Gonzalez-Badillo,B., Veri,A.O.,
Knight,J.D.R., Rajendran,D., Couzens,A.L., Currie,K.W., Tkach,J.M.,
Cheung,S.W.T., Pelletier,L. and Gingras,A.C.
TITLE Phenotypic and Interaction Profiling of the Human Phosphatases
Identifies Diverse Mitotic Regulators
JOURNAL Cell Rep 17 (9), 2488-2501 (2016)
PUBMED 27880917
REFERENCE 4 (bases 1 to 3857)
AUTHORS Chen,G.I., Tisayakorn,S., Jorgensen,C., D'Ambrosio,L.M.,
Goudreault,M. and Gingras,A.C.
TITLE PP4R4/KIAA1622 forms a novel stable cytosolic complex with
phosphoprotein phosphatase 4
JOURNAL J Biol Chem 283 (43), 29273-29284 (2008)
PUBMED 18715871
REMARK GeneRIF: PP4R4 forms a novel cytosolic complex with PP4c,
independent from the complexes containing PP4R1, PP4R2.PP4R3, and
alpha4, and the regulatory subunits of PP4c have evolved different
modes of interaction with the catalytic subunit
REFERENCE 5 (bases 1 to 3857)
AUTHORS Namciu,S.J., Friedman,R.D., Marsden,M.D., Sarausad,L.M.,
Jasoni,C.L. and Fournier,R.E.
TITLE Sequence organization and matrix attachment regions of the human
serine protease inhibitor gene cluster at 14q32.1
JOURNAL Mamm Genome 15 (3), 162-178 (2004)
PUBMED 15014966
REFERENCE 6 (bases 1 to 3857)
AUTHORS Andrade,M.A., Petosa,C., O'Donoghue,S.I., Muller,C.W. and Bork,P.
TITLE Comparison of ARM and HEAT protein repeats
JOURNAL J Mol Biol 309 (1), 1-18 (2001)
PUBMED 11491282
REMARK Review article
REFERENCE 7 (bases 1 to 3857)
AUTHORS Groves,M.R., Hanlon,N., Turowski,P., Hemmings,B.A. and Barford,D.
TITLE The structure of the protein phosphatase 2A PR65/A subunit reveals
the conformation of its 15 tandemly repeated HEAT motifs
JOURNAL Cell 96 (1), 99-110 (1999)
PUBMED 9989501
COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from DB480905.1, BC068491.1 and
AB046842.1.
On Nov 23, 2018 this sequence version replaced NM_058237.1.
Summary: The protein encoded by this gene is a HEAT-like
repeat-containing protein. The HEAT repeat is a tandemly repeated,
37-47 amino acid long module occurring in a number of cytoplasmic
proteins. Arrays of HEAT repeats form a rod-like helical structure
and appear to function as protein-protein interaction surfaces. The
repeat-containing region of this protein has some similarity to the
constant regulatory domain of the protein phosphatase 2A PR65/A
subunit. The encoded protein binds protein serine/threonine
phosphatase 4c in the cytoplasm. [provided by RefSeq, Jan 2017].
Transcript Variant: This variant (1) encodes the longest isoform
(1).
##Evidence-Data-START##
Transcript exon combination :: SRR1803613.137306.1,
SRR1660803.23201.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns
SAMEA1968968, SAMEA2145245
[ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
MANE Ensembl match :: ENST00000304338.8/ ENSP00000305924.3
RefSeq Select criteria :: based on conservation, expression,
longest protein
##RefSeq-Attributes-END##
COMPLETENESS: full length.
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-31 DB480905.1 11-41
32-2879 BC068491.1 1-2848
2880-3137 AB046842.1 2809-3066
3138-3411 BC068491.1 3106-3379
3412-3857 AB046842.1 3340-3785
FEATURES Location/Qualifiers
source 1..3857
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="14"
/map="14q32.12-q32.13"
gene 1..3857
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/note="protein phosphatase 4 regulatory subunit 4"
/db_xref="GeneID:57718"
/db_xref="HGNC:HGNC:23788"
/db_xref="MIM:616790"
exon 1..261
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
misc_feature 85..87
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/note="upstream in-frame stop codon"
CDS 145..2766
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/note="isoform 1 is encoded by transcript variant 1;
HEAT-like repeat-containing protein;
serine/threonine-protein phosphatase 4 regulatory subunit
4; cilia and flagella associated protein 14"
/codon_start=1
/product="serine/threonine-protein phosphatase 4
regulatory subunit 4 isoform 1"
/protein_id="NP_478144.1"
/db_xref="CCDS:CCDS9921.1"
/db_xref="GeneID:57718"
/db_xref="HGNC:HGNC:23788"
/db_xref="MIM:616790"
/translation="MHPPPPAAAMDFSQNSLFGYMEDLQELTIIERPVRRSLKTPEEI
ERLTVDEDLSDIERAVYLLSAGQDVQGTSVIANLPFLMRQNPTETLRRVLPKVREALH
VAGVEMQLTAAMSFLTILQDESVSIHAYTHSFLQVILLHLEHRDTGVSNAWLETLLSV
IEVLPKETLRHEILNPLVSKAQLSQTVQSRLVSCKILGKLTNKFDAHTIKREILPLVK
SLCQDVEYEVRSCMCRQLENIAQGIGTELTKSVVLPELIELSRDEGSSVRLAAFETLV
NLLDIFDTDDRSQTILPLVKSFCEKSFKADESILISLSFHLGKLCHGLYGIFTPDQHL
RFLEFYKKLCTLGLQQENGHNENQIPPQILEQEKKYISVRKNCAYNFPAMIVFVDPKN
FHMELYSTFFCLCHDPEVPVRYTIAICFYEVSKLLNSGVYLIHKELITLLQDESLEVL
DALIDHLPEILELMSTGGESSVQENKLSSLPDLIPALTAAEQRAAASLKWRTHEKLLQ
KYACLPHVISSDQIYYRFLQRMFTIMMTNNVLPVQKAASRTLCIFLRYNRKQEQRHEV
IQKLIEQLGQGKSYWNRLRFLDTCEFIIEIFSKSFFCKYFFLPAIELTHDPVANVRMK
LCYLLPKVKSTLKIPADKHLLQQLEMCVRKLLCQEKDKDVLAIVKRTVLELDRMEMSM
DAFQKKFYEKDLLDQEKEREELLLLEMEQLEKEKQQNDGRPMSDKMFEKKRRDTKTPT
QSLPKNIPISVPGPSSVTPSTSKEIKKSKLIRSQSFNNQAFHAKYGNLEKCASKSSTT
GYTTSVSGLGKTSVLSLADDSFRTRNASSVPSSFSPNTPLPSTSRGTGNSVDPKSSGS
KDTQPRKATLKSRKSNP"
misc_feature 757..873
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/note="Region: HEAT-like repeat region"
misc_feature 781..897
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/note="propagated from UniProtKB/Swiss-Prot (Q6NUP7.1);
Region: HEAT 1"
misc_feature 874..990
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/note="Region: HEAT-like repeat region"
misc_feature 898..1014
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/note="propagated from UniProtKB/Swiss-Prot (Q6NUP7.1);
Region: HEAT 2"
misc_feature 1318..1425
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/note="propagated from UniProtKB/Swiss-Prot (Q6NUP7.1);
Region: HEAT 3"
misc_feature 2281..2442
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/note="propagated from UniProtKB/Swiss-Prot (Q6NUP7.1);
Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
misc_feature 2467..2469
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/note="Phosphoserine.
/evidence=ECO:0000250|UniProtKB:Q8C0Y0; propagated from
UniProtKB/Swiss-Prot (Q6NUP7.1); phosphorylation site"
misc_feature 2533..2535
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/note="Phosphothreonine.
/evidence=ECO:0000250|UniProtKB:Q8C0Y0; propagated from
UniProtKB/Swiss-Prot (Q6NUP7.1); phosphorylation site"
misc_feature 2608..2763
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/note="propagated from UniProtKB/Swiss-Prot (Q6NUP7.1);
Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
exon 262..335
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 336..438
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 439..586
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 587..660
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 661..767
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 768..875
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 876..997
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 998..1120
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 1121..1290
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 1291..1410
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 1411..1488
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 1489..1572
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 1573..1755
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 1756..1861
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 1862..2009
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 2010..2154
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 2155..2196
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 2197..2271
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 2272..2341
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 2342..2428
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 2429..2522
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 2523..2593
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 2594..2741
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
exon 2742..3857
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/inference="alignment:Splign:2.1.0"
regulatory 3836..3841
/regulatory_class="polyA_signal_sequence"
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/note="hexamer: AATAAA"
polyA_site 3857
/gene="PPP4R4"
/gene_synonym="CFAP14; KIAA1622; PP4R4"
/note="major polyA site"
ORIGIN
1 cttgctcccg ccgcctggca gcctcacgct cggctccagc ggccaagagc cggagaaagt
61 cctgctggtg ggcggccgcg gggctgaggg cgtccggcat cccggggccg ctccggcccg
121 ggcggcgaga gtgcccggcg gtccatgcat ccgccgccgc ccgccgccgc gatggatttc
181 agtcagaaca gcctgttcgg ttacatggag gacctgcagg agctcaccat catcgagagg
241 ccggtccgcc ggagcctcaa gacaccggaa gaaatagaaa gattgacagt cgatgaagac
301 ctcagtgata ttgaaagggc tgtttatctg ctcagtgctg gtcaagatgt ccaaggaaca
361 agtgtgattg caaatctccc atttttgatg cgacagaatc ccactgagac gcttcggaga
421 gtgttgccaa aagtcagaga agccctgcat gttgcaggag tggaaatgca gttaacggct
481 gcgatgtcat ttctgaccat tctgcaggac gaatcagtgt caattcatgc atatacccac
541 tcattcctcc aagtcattct cctgcatctg gagcacaggg acacaggtgt cagcaatgca
601 tggctggaaa ctcttctgtc tgttatagaa gtattgccaa aagaaaccct acggcatgag
661 attttgaatc cacttgtttc caaggcacaa ctttcccaaa cagtccagtc tcgtttagtt
721 agttgtaaaa ttttaggaaa attgaccaac aaatttgatg cccacaccat taagcgagaa
781 atacttcctc tggtaaaatc actctgtcaa gatgtagaat atgaagttcg atcttgtatg
841 tgtcggcaat tagaaaatat agcccagggc attgggacag aacttacaaa aagtgtggtg
901 ctccctgaat taatagaact ttctagggat gaaggcagca gtgtacgact tgcagctttt
961 gaaactttgg ttaatctgct tgatatattt gatacagatg acagaagtca aactatactt
1021 cccttagtga aatcattttg tgaaaaatct ttcaaagcag atgaatcaat tcttatttct
1081 ttatctttcc atttaggaaa actatgtcat ggactatatg gaattttcac tccagatcag
1141 cacttgagat ttttggaatt ttataagaaa ctttgtacat tgggtttgca acaagaaaat
1201 ggacacaatg aaaaccagat tccaccccaa atcctagagc aggagaagaa atatatttca
1261 gtacggaaga actgtgctta taactttccg gccatgattg tttttgttga tcctaaaaac
1321 ttccacatgg aactctattc tacattcttc tgcctttgcc atgaccctga agtaccagtc
1381 agatacacta ttgctatttg cttttatgaa gtatctaagc ttctgaattc tggagtatat
1441 ttaatacata aagaactaat aacattatta caagatgaat cactggaggt actagatgct
1501 cttatagatc atcttccaga aatcttggaa cttatgtcta ctggtggaga aagcagtgtt
1561 caagaaaata agttatcttc tctgcctgac ttgattccag cactcacagc tgctgaacag
1621 cgagctgcag cctctttaaa atggagaact catgagaagc tacttcagaa atatgcctgc
1681 ctgccacatg tcatatcaag cgatcagatt tattaccgtt tcttacaaag aatgttcaca
1741 atcatgatga caaataatgt tttacctgtc caaaaggcgg cttcacgaac tctatgcatt
1801 tttctgcgtt ataatcgtaa acaagaacag agacatgagg tcattcaaaa attaattgaa
1861 caattgggcc aaggaaaaag ttactggaat agacttcgat ttttggatac ctgtgaattt
1921 attatagaga tattttcaaa atcatttttc tgtaaatatt tctttctacc tgctattgaa
1981 ctgacacatg atccagtagc aaatgtgaga atgaaacttt gctacctgtt gcccaaagtg
2041 aaatctactc tgaagattcc tgctgataag catctacttc agcagttaga aatgtgtgtg
2101 aggaaactcc tgtgtcaaga aaaagataaa gatgttctgg ctattgtaaa aagaactgta
2161 ttagagttgg acagaatgga aatgtctatg gatgcttttc agaaaaagtt ttatgagaaa
2221 gatttgttgg atcaagagaa agaaagagaa gaactacttc ttttggaaat ggaacaatta
2281 gagaaagaaa agcaacagaa tgatggaagg cccatgagtg ataaaatgtt tgaaaagaaa
2341 cgtagagaca ctaagacacc aacgcaaagt ctgcccaaga acatccccat ttctgttcct
2401 ggaccctctt ctgtcacccc atcgacaagt aaagaaatca agaaatccaa actgattcga
2461 agccagtctt ttaataatca agcttttcat gcaaaatatg gcaacttaga gaaatgtgct
2521 agtaaaagtt ctacaacagg atatacaact tctgtctcag ggttaggaaa gacttctgtg
2581 ctttcactag ctgatgattc attccggact cgtaatgcca gtagcgttcc atcttccttt
2641 tctcctaata ctcccttacc gagtacttcc cgtgggacag gtaactcagt tgaccccaag
2701 agcagtggaa gtaaagatac acaaccacgg aaggctacct taaaatccag aaaatccaat
2761 ccttaaatca actgcttgat gaaggaggca aaacaaaggc agcaggagat aatgtgattg
2821 gtacacaaaa gctaaagcag tggctggggc tttgttttta aattttgggt tttttttttt
2881 gttgttgtta atgagcagaa agagagacat aatgacagct gatgttaaac ttttcatatt
2941 tcaaattaga ttccctagga ggtataatat atatttcttg agtaataatg tggttacgga
3001 attccaatgt tatagtgaag tgtaatgaaa aacatctcta ggaatgtgct ttaaccactg
3061 ctgcaaaaga gacaagtctg catttatttg tgcaggaaac cagccattta attgttctag
3121 agttttagca tttaaaaatc gtatgaaagt ctacatcagc tgaattgtcc tagcttgata
3181 agcacttgga gggggacttg gaaggtgaga aagatactgc attttctcat gagtctccaa
3241 gcccacttga aaagtcacac tgaaaggatg caaatagccg tctgcgattt tggcggccct
3301 ccggattgtg gtgacaatat gttgtacccg gacattccca aatttaaatt tggcagtgaa
3361 aattccacaa agatttctgg taactttgag ctataaatac cttaaaaata atagattgat
3421 gattttcttt atttcctaga gaatttattt tataaatact ttgtttacat tttagattgt
3481 acttgtcttt atttaaaatg atgaatctag tctgaatcag ctgttattcc aagctatcat
3541 gcttaagcta tgtcaacagc atttatttgt actaatgcta atatttccat caaaatttgc
3601 ctgtggaata tatacagttc ttaattttaa actgtcagtt cttgagatat accgtgtgaa
3661 gctattgtca gcttctctat ttcaaaatgc aatcagcata taactatatt gatattagaa
3721 gatatttgtt ttttaaaata tattgatgta tgataaagat gatctaattt gtgaatgcat
3781 atgtatgtgt ggttactttt tataatgtga aataatgaat aatgaattta ctcaaaataa
3841 aacataggtt aatgaga
//