LOCUS NZ_ATMJ01000032 27620 bp DNA linear CON 26-APR-2024
DEFINITION Tatumella ptyseos ATCC 33301 contig000032, whole genome shotgun
sequence.
ACCESSION NZ_ATMJ01000032 NZ_ATMJ01000000
VERSION NZ_ATMJ01000032.1
DBLINK BioProject: PRJNA224116
BioSample: SAMN02199042
Assembly: GCF_000439895.1
KEYWORDS WGS; RefSeq.
SOURCE Tatumella ptyseos ATCC 33301
ORGANISM Tatumella ptyseos ATCC 33301
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
Enterobacterales; Erwiniaceae; Tatumella.
REFERENCE 1 (bases 1 to 27620)
AUTHORS Tracz,D.M., Gilmour,M.W., Bernard,K.A., Mabon,P., Beniac,D.R.,
Hoang,L., Paccagnella,A., Kibsey,P., Van Domselaar,G.,
Westmacott,G.R., Tabor,H. and Corbett,C.R.
TITLE A comprehensive, polyphasic taxonomic analysis of Tatumella
saanichensis species novum, a bacterium isolated from a Cystic
fibrosis patient
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 27620)
AUTHORS Tracz,D.M., Gilmour,M.W., Bernard,K.A., Mabon,P., Beniac,D.R.,
Hoang,L., Paccagnella,A., Kibsey,P., Van Domselaar,G.,
Westmacott,G.R., Tabor,H. and Corbett,C.R.
TITLE Direct Submission
JOURNAL Submitted (24-JUN-2013) Bioforensics Assay Development and
Diagnostics, National Microbiology Laboratory, 1015 Arlington St.,
Winnipeg, Manitoba R2E3R2, Canada
COMMENT REFSEQ INFORMATION: The reference sequence is identical to
ATMJ01000032.1.
The annotation was added by the NCBI Prokaryotic Genome Annotation
Pipeline (PGAP). Information about PGAP can be found here:
https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
##Genome-Assembly-Data-START##
Assembly Method :: Velvet v. 1.2.08
Assembly Name :: atcc_33301_v1
Genome Coverage :: 33.0x
Sequencing Technology :: Illumina
##Genome-Assembly-Data-END##
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI RefSeq
Annotation Name :: GCF_000439895.1-RS_2024_04_26
Annotation Date :: 04/26/2024 00:55:34
Annotation Pipeline :: NCBI Prokaryotic Genome
Annotation Pipeline (PGAP)
Annotation Method :: Best-placed reference protein
set; GeneMarkS-2+
Annotation Software revision :: 6.7
Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA
Genes (total) :: 3,338
CDSs (total) :: 3,253
Genes (coding) :: 3,185
CDSs (with protein) :: 3,185
Genes (RNA) :: 85
rRNAs :: 6, 1, 2 (5S, 16S, 23S)
complete rRNAs :: 4 (5S)
partial rRNAs :: 2, 1, 2 (5S, 16S, 23S)
tRNAs :: 69
ncRNAs :: 7
Pseudo Genes (total) :: 68
CDSs (without protein) :: 68
Pseudo Genes (ambiguous residues) :: 0 of 68
Pseudo Genes (frameshifted) :: 24 of 68
Pseudo Genes (incomplete) :: 58 of 68
Pseudo Genes (internal stop) :: 11 of 68
Pseudo Genes (multiple problems) :: 20 of 68
##Genome-Annotation-Data-END##
FEATURES Location/Qualifiers
source 1..27620
/organism="Tatumella ptyseos ATCC 33301"
/mol_type="genomic DNA"
/submitter_seqid="contig000032"
/strain="ATCC 33301"
/isolation_source="sputum"
/host="Homo sapiens"
/specimen_voucher="ATCC 33301"
/culture_collection="ATCC:33301"
/type_material="type strain of Tatumella ptyseos"
/db_xref="taxon:1005995"
/geo_loc_name="USA: Baltimore"
gene <1..189
/gene="tuf"
/locus_tag="DW48_RS18230"
/pseudo
CDS <1..189
/gene="tuf"
/locus_tag="DW48_RS18230"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_012147227.1"
/note="EF-Tu; promotes GTP-dependent binding of
aminoacyl-tRNA to the A-site of ribosomes during protein
biosynthesis; when the tRNA anticodon matches the mRNA
codon, GTP hydrolysis results; the inactive EF-Tu-GDP
leaves the ribosome and release of GDP is promoted by
elongation factor Ts; many prokaryotes have two copies of
the gene encoding EF-Tu; incomplete; too short partial
abutting assembly gap; missing N-terminus; Derived by
automated computational analysis using gene prediction
method: Protein Homology."
/pseudo
/codon_start=1
/transl_table=11
/product="elongation factor Tu"
gene 413..796
/gene="secE"
/locus_tag="DW48_RS0110160"
CDS 413..796
/gene="secE"
/locus_tag="DW48_RS0110160"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_007665874.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="preprotein translocase subunit SecE"
/protein_id="WP_025902285.1"
/translation="MSANTEAQRSGRGLEIVKWLVVAVLVAVAIYGNYHYRNVNLPLR
ALVVVVIIAVAGAIALLTTKGKAAVAFAREARTEVRKVIWPTRQETLQTTLIVAAVTA
VMSLILWGLDGILVRLVSFITGLRF"
gene 798..1343
/gene="nusG"
/locus_tag="DW48_RS0110165"
CDS 798..1343
/gene="nusG"
/locus_tag="DW48_RS0110165"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_004953928.1"
/GO_process="GO:0006353 - DNA-templated transcription
termination [Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="transcription termination/antitermination
protein NusG"
/protein_id="WP_025902284.1"
/translation="MSEAPKKRWYVVQAFSGFESRVAVSLREHIKLHNMEDLFGDVMV
PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMEDASWHLVRSVPRVMGFIGGTSDRPAP
ISDKEVDAIMNRLQQAGDKPRPKTMFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
SVSIFGRATPVELDFGQVEKG"
gene 1485..1913
/gene="rplK"
/locus_tag="DW48_RS0110170"
CDS 1485..1913
/gene="rplK"
/locus_tag="DW48_RS0110170"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_006121495.1"
/GO_component="GO:0022625 - cytosolic large ribosomal
subunit [Evidence IEA]; GO:0000315 - organellar large
ribosomal subunit [Evidence IEA]"
/GO_function="GO:0003735 - structural constituent of
ribosome [Evidence IEA]"
/GO_process="GO:0006412 - translation [Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="50S ribosomal protein L11"
/protein_id="WP_025902283.1"
/translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
KTESLEKGLPTPVVITVYSDRSFTFITKTPPAAVLLKKAAGIKSGSGKPNKDKVGKVT
RAQLQEIATTKAADMTGADLDAMTRSIEGTAISMGLVVED"
gene 1917..2621
/gene="rplA"
/locus_tag="DW48_RS0110175"
CDS 1917..2621
/gene="rplA"
/locus_tag="DW48_RS0110175"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_004154987.1"
/GO_component="GO:0022625 - cytosolic large ribosomal
subunit [Evidence IEA]; GO:0000311 - plastid large
ribosomal subunit [Evidence IEA]"
/GO_function="GO:0003735 - structural constituent of
ribosome [Evidence IEA]"
/GO_process="GO:0006412 - translation [Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="50S ribosomal protein L1"
/protein_id="WP_025902282.1"
/translation="MAKLTKRMRTIRDKVDVTKQYEITEAVALLKELATAKFVESVDV
SVNLGIDARKSDQNVRGATVLPNGTGRSVRVAVFAQGANAEAAKAAGAELVGMEDLAD
QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTSAKGVYIKKVSLSTTM
GAGVAIDQAGLSTQTA"
gene 2972..3469
/gene="rplJ"
/locus_tag="DW48_RS0110180"
CDS 2972..3469
/gene="rplJ"
/locus_tag="DW48_RS0110180"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_002438625.1"
/GO_component="GO:0015934 - large ribosomal subunit
[Evidence IEA]"
/GO_function="GO:0003735 - structural constituent of
ribosome [Evidence IEA]"
/GO_process="GO:0006412 - translation [Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="50S ribosomal protein L10"
/protein_id="WP_025902281.1"
/translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
GREAGVYMRVVRNTLLRRVVEGTPFECLKDTFVGPTLIAYSMEHPGAAARLFKDFAKA
NAKFEVKAAAFEGELIPAAQIDRLATLPTYEEALARLMSTMKEAAAGKLVRTLAAVRD
AKEAA"
gene 3533..3898
/gene="rplL"
/locus_tag="DW48_RS0110185"
CDS 3533..3898
/gene="rplL"
/locus_tag="DW48_RS0110185"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_005122872.1"
/GO_component="GO:0022625 - cytosolic large ribosomal
subunit [Evidence IEA]; GO:0000315 - organellar large
ribosomal subunit [Evidence IEA]"
/GO_function="GO:0003735 - structural constituent of
ribosome [Evidence IEA]"
/GO_process="GO:0006412 - translation [Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="50S ribosomal protein L7/L12"
/protein_id="WP_025902280.1"
/translation="MSITKDQILEAVAAMSVMEVVELVSAMEEKFGVSAAAAVAVAAG
PAEAAEEQTEFDVILKSVGGNKVAVIKAVRGATGLGLKEAKDLVESAPAAIKEAISKD
DAAALEAALKEAGAEVEIK"
gene 4230..8258
/gene="rpoB"
/locus_tag="DW48_RS0110190"
CDS 4230..8258
/gene="rpoB"
/locus_tag="DW48_RS0110190"
/EC_number="2.7.7.6"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_004870488.1"
/GO_component="GO:0000345 - cytosolic DNA-directed RNA
polymerase complex [Evidence IEA]"
/GO_function="GO:0003899 - DNA-directed 5'-3' RNA
polymerase activity [Evidence IEA]"
/GO_process="GO:0006351 - DNA-templated transcription
[Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="DNA-directed RNA polymerase subunit beta"
/protein_id="WP_025902279.1"
/translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVKECQIRGVTFSAPLRVKLRL
VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
QIIDLFFDKVVYEIRDNKLQMALVPERLRGETASFDIESNGTVYVEKGRRITARHIRQ
LEKDNVQHIEVPVEYIAGKVVARDYIDESTGELIVAANMELSMDLLAKLSQSGHKRIE
TLFTNDLDHGPYISETLRVDPTSDRLSALVEIYRMMRPGEPPTREAAENLFENLFFSE
DRYDLSAVGRMKFNRSLLRDEIEGAGILSHDDIIQVMKKLIDIRNGIGEVDDIDHLGN
RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
PNIGLINSLSVYAQTNEYGFLETPYRRVVDSQVTDEIHYLSAIEEGNFVIAQANTNLD
DDGRFVDDLVTCRNKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGTVQYVDASRIVIKVNEDE
MYAGEAGIDIYNLTKYTRSNQNTCINQMPCVNLGEPIERGDVLADGPSTDLGELALGQ
NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLTEELQILEAGLF
TRIYTVLTTGGVEAEKLDKLPRERWLELGLTDEEKQNQLEQLAEQYDELKREFEKKLE
AKRRKITQGDDLAPGVLKIVKVYLAVKRQIQPGDKMAGRHGNKGVISKINPIEDMPYD
ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQDVARLREFIQ
RAYDLGTDVRQKVDLNTFSDDQVLRLAENLKKGMPIATPVFDGAKESEIKELLTLGGI
PTSGQITLFDGRTGEQFERQVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
ESFNVLLKEIRSLGINIELEDE"
gene 8339..12559
/gene="rpoC"
/locus_tag="DW48_RS0110195"
CDS 8339..12559
/gene="rpoC"
/locus_tag="DW48_RS0110195"
/EC_number="2.7.7.6"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_006121501.1"
/GO_component="GO:0000345 - cytosolic DNA-directed RNA
polymerase complex [Evidence IEA]"
/GO_function="GO:0003899 - DNA-directed 5'-3' RNA
polymerase activity [Evidence IEA]"
/GO_process="GO:0006351 - DNA-templated transcription
[Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="DNA-directed RNA polymerase subunit beta'"
/protein_id="WP_025902278.1"
/translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRDRM
GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLEKRQIL
TEEQYLDALEEFGDEFDAKMGAEAVQALLKGMDLEQECEQLREELNETNSETKRKKLT
KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
QGRFRQNLLGKRVDYSGRSVITVGPYLHLHQCGLPKKMALELFKPFIYGKLELRGLAT
TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
GLYYMTRDKVNAKGEAMVLTGPKEAERVYRAGLAELHARVKVRITEYSKNEQGEFIPH
TSLVDTTVGRAILWMIVPKGLPYSIINQALGKKAISKMLNTCYRILGLKPTVIFADQT
MYTGFAYAARSGASVGIDDMVIPAKKAEIITEAEAEVAEIQEQFQSGLVTAGERYNKV
IDIWAAANERVSKAMMENLQTETVINRHGEEEQQVSFNSIYMMADSGARGSAAQIRQL
AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRERVLGRVTAEDVLKPGSADI
LVARNTLLDEHWCDVLEQNSVDSVKVRSVVGCTTDFGVCAHCYGRDLARGHIINKGEA
VGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGTIKLINAKSVTNSAGK
LVITSRNVELNMIDEFGRTKESYKVPYGSVMAKGDGEQANAGETVANWDPHTMPVITE
VNGYVRFVDMNDGQTITRQTDELTGLSSIVVMDTAERTSGGKDLRPALKVVDANGDDV
MLPGTDMPAQYFLPGNAIVQLEDGVKISSGDTLARVPQESGGTKDITGGLPRVADLFE
ARRPKEPAILAEISGIISFGKETKGKRRLVITPVDGSEPYEEMIPKWRQLNVFEGERV
ERGDVVSDGPESPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
KATIASSGSSEFLEGEQAEVSRVMIANRELEANGKVPATFARDLLGITKASLATESFI
SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRHAGEA
PVVPQVTADEASASLAELLNAGLGGNDE"
gene 12639..13700
/locus_tag="DW48_RS0110200"
CDS 12639..13700
/locus_tag="DW48_RS0110200"
/inference="COORDINATES: protein motif:HMM:NF018389.4"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="DUF1176 domain-containing protein"
/protein_id="WP_025902277.1"
/translation="MKRCCLPAILFSMLVVTFGARASEPVQRMFQDWQVTCNNLNDCD
IRNVDENMRVVIRHEAGPRGAVSLDLMGFDIDKPSGIWLDGKLWNNSLTPVYADNKHD
YAGYHTTSLQAVQAFAEAAKNAGRIALTPDEDDDTGSLKGLNTSLHFADEIQGRLNNR
TAFIRTGNGIPAQVPLRYAFEIPATKIPAIFPVGDGNALINEVLQTQQDVLDDEECSP
DADDITRSQVKPLDKKHVLVMINCVTGAYQSSGIVFIVPRAHPDRGSEVELTLPLKDD
QGDPQTITWFTDVSYDPKTALLTYIARGHGVSDCGESGTWRYDGHVFQLMSYHNQPTC
DGGEPGHWPSVWLTPGFKE"
gene complement(13730..14857)
/gene="thiH"
/locus_tag="DW48_RS0110205"
CDS complement(13730..14857)
/gene="thiH"
/locus_tag="DW48_RS0110205"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_000847560.1"
/GO_function="GO:0016829 - lyase activity [Evidence IEA];
GO:1904047 - S-adenosyl-L-methionine binding [Evidence
IEA]"
/GO_process="GO:0009228 - thiamine biosynthetic process
[Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="2-iminoacetate synthase ThiH"
/protein_id="WP_029990740.1"
/translation="MPDFADYWQTLDQNALRLQIDSQSPADVERALTCHKPGITELMA
LLSPAAEQYLEPLAEKALQLTRQRFGNTVNFFAPLYLSNLCANDCTYCGFSMSNRIRR
KTLDSREIGLECDALRQLGFNQVLLVTGEHQTKVGMDYFRQHLPDIRRRFSSLMMEVQ
PLQEQEYRELHQLGLDGVLVYQETYHASCYATHHLRGNKQDFLFRLQTPERLGRAGID
KIGLGVLAGLSGHWRTDCLMMGEHLNWLQKYYWRSRFSVAFPRLRPCAGGITPASQMS
ERQLLQTMCAFRLFSPQTDISLSTRESPVFRDHAIPITVTTASAFSKTTPGGYASDTD
ALEQFSPDDHRRPEVVAAALQQAGLQPVWKDWEFAFGRESA"
gene complement(14850..15638)
/locus_tag="DW48_RS0110210"
CDS complement(14850..15638)
/locus_tag="DW48_RS0110210"
/EC_number="2.8.1.10"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_004957757.1"
/GO_function="GO:1990107 - thiazole synthase activity
[Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="thiazole synthase"
/protein_id="WP_029990742.1"
/translation="MLKIADKTFSSRLLTGTGKFASPELMLAALRASGSQLATLAMKR
IDFGDNRDAILEPLRRSGVTLLPNTSGAKTAGEAVFAARLAREALGTHWVKLEIHPDS
RYLLPDPIETLKAAEQLVREGFTVLPYCGADPVLCKRLAEAGCAAVMPLGAPIGSNRG
LLSRDFLHIIIEQATVPVIIDAGIGVPSDACEAMECGADAVLVNTAIATARDPVSAAR
AFALATEAGYLCRGQVTTTSTQAKASSPLTAFLDTDNAEAATHA"
gene complement(15641..15841)
/gene="thiS"
/locus_tag="DW48_RS0110215"
CDS complement(15641..15841)
/gene="thiS"
/locus_tag="DW48_RS0110215"
/inference="COORDINATES: protein motif:HMM:NF014636.4"
/GO_function="GO:0003824 - catalytic activity [Evidence
IEA]"
/GO_process="GO:0009228 - thiamine biosynthetic process
[Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="sulfur carrier protein ThiS"
/protein_id="WP_025902274.1"
/translation="MQIKVNDNVISVESSCPVNRLLHTLSVAVQGTALAVNERIIPAS
EWAGYRLQENDDVVIFQVIAGG"
gene complement(15831..16583)
/locus_tag="DW48_RS0110220"
CDS complement(15831..16583)
/locus_tag="DW48_RS0110220"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_010342220.1"
/GO_function="GO:0008641 - ubiquitin-like modifier
activating enzyme activity [Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="HesA/MoeB/ThiF family protein"
/protein_id="WP_025902273.1"
/translation="MDDKSFMRYSRQLLLEEIGPEGQQKLSDARILIVGLGGLGSPAA
LYLAAAGAGTLLLADDDDLHISNLQRQILYRSSDTGISKAKLAAEALRALNPSITLHA
ISDRIPLSAGDELPARPTLVLDCSDNLATRHRLNAWCFSHNLPLITASAIGFHGQLLV
LTPPWQHGCYQCLWPDSREPERNCRNSGIAGPVTGVMGSLQALEAIKWICSGRNTAAG
QLILFDGLALRWRKLKLTPERNCPICGARHAN"
gene complement(16576..17226)
/gene="thiE"
/locus_tag="DW48_RS0110225"
CDS complement(16576..17226)
/gene="thiE"
/locus_tag="DW48_RS0110225"
/EC_number="2.5.1.3"
/inference="COORDINATES: similar to AA
sequence:RefSeq:YP_005224512.1"
/GO_function="GO:0004789 - thiamine-phosphate
diphosphorylase activity [Evidence IEA]"
/GO_process="GO:0009228 - thiamine biosynthetic process
[Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="thiamine phosphate synthase"
/protein_id="WP_051170818.1"
/translation="MTQIFPPVPRYCGLYAIVDSTEWIENLLNAGVMTLQLRMKQGSA
AEKEKAIIDSIRLARGFGARLFINDEWQLAIKHGAYGVHLGQEDLQTASLSAIHQAGL
RLGVSTHNTAEIDRVLPLHPSYIALGHIFPTTTKEMPSSPQGLANLRRHVLRLSGIPT
VAIGGISLDRAGEVLATGVGSIAVVSAITRAADWRQAVRQLQQKVSDAREVTTDAG"
gene complement(17223..19148)
/gene="thiC"
/locus_tag="DW48_RS0110230"
CDS complement(17223..19148)
/gene="thiC"
/locus_tag="DW48_RS0110230"
/EC_number="4.1.99.17"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_008108410.1"
/GO_function="GO:0051536 - iron-sulfur cluster binding
[Evidence IEA]"
/GO_process="GO:0009228 - thiamine biosynthetic process
[Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="phosphomethylpyrimidine synthase ThiC"
/protein_id="WP_029990745.1"
/translation="MSEPLSRREKREQAKNFINSLNSEFFPGSERRWITGSRPDIRVP
VRAILLSPTRVGGTPDNPVMEENEPIPVYDTSGPFGDNTTVPDIHHGIRKLRQVWIDE
RQDSEPLPSTSSSYTRQRLADESLDSLRFQHLPEPRKAKKGRCVTQLHYARQGIITPE
MEFIALRENMGREQVTRPELLQQHPGKHFFAPLPASITPEFVRQEVAAGRAIIPANIN
HPESEPMIIGRNFLVKINANIGNSAVTSSIEEEVEKLVWATRWGADTLMDLSTGRNIH
ETREWILRNSPIPVGTVPIYQALEKVNGVAEDLNWAVFRDTLLEQAEQGVDYFTIHAG
VLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYRHFREICEICASYDIALSLG
DGLRPGSIQDANDEAQFAELHTLGELTKIAHEYDVQVMIEGPGHVPMHMIQRNMTEQL
EHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPDKED
VKQGLIAYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFHLALDPETARSYHD
QTLPQESGKVAHFCSMCGPKFCSMKISQEVRDFASKNPAPEVVIDEGMLEMSRRFREK
GSEIYTPVPAARTGAKA"
regulatory complement(19236..19336)
/regulatory_class="riboswitch"
/inference="COORDINATES: nucleotide
motif:Rfam:14.4:RF00059"
/inference="COORDINATES: profile:INFERNAL:1.1.5"
/note="TPP riboswitch; Derived by automated computational
analysis using gene prediction method: cmsearch."
/bound_moiety="thiamine pyrophosphate"
/db_xref="RFAM:RF00059"
gene 19574..20347
/gene="nudC"
/locus_tag="DW48_RS0110235"
CDS 19574..20347
/gene="nudC"
/locus_tag="DW48_RS0110235"
/EC_number="3.6.1.22"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_004388101.1"
/GO_function="GO:0016787 - hydrolase activity [Evidence
IEA]; GO:0000210 - NAD+ diphosphatase activity [Evidence
IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="NAD(+) diphosphatase"
/protein_id="WP_029990746.1"
/translation="MEREITRNDRGWWVISQQNKLWLPQGTLPHGSAAEFALTGSRAL
QTGEWQGEPVWLVRETRAQDMMSLRQLLGEESGLFRMAGRAVQLSEFYRSHRYCGYCG
AEMHLSRSEFACLCGSCHERYYPQIAPCIIVAIRRQDKILLANHVRSRNGIYTALAGF
AEVGETLEQTVAREVMEESNIRIKNLRYVASQPWPFPQSLMMAFLADYDSGELRIDPK
ELQDAGWYRYDALPVLPPPGTIARRLIEDTIALCRSENE"
gene 20432..21496
/gene="hemE"
/locus_tag="DW48_RS0110240"
CDS 20432..21496
/gene="hemE"
/locus_tag="DW48_RS0110240"
/EC_number="4.1.1.37"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_017460013.1"
/GO_function="GO:0004853 - uroporphyrinogen decarboxylase
activity [Evidence IEA]"
/GO_process="GO:0006779 - porphyrin-containing compound
biosynthetic process [Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="uroporphyrinogen decarboxylase"
/protein_id="WP_025902269.1"
/translation="MSELKNDRYLRALLRQPTDVTPVWMMRQAGRYLPEYKATRAEAG
DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTIPDAMGLGLYFETGEGPRFSSP
ITCAADVKKLPVPDPEQELQYVMNAVRTIRKNLKGEVPLIGFSGSPWTLATYMVEGGS
SKAFTKIKKMMYSDPSTLHAMLDKLAESVILYLNAQIRAGAQSVMVFDTWGGVLTGRD
YRDFSLYYMHKIVDGLLHENEGRRVPVTLFTKGGGQWLEQIADTGCDAIGLDWTTEIG
NARERVGHRVALQGNMDPSVLYAPPARIEQEVSSILEGFGQGDGHVFNLGHGIHQDVP
PEHAGAFVEAVHRLSAAYHK"
gene 21499..22170
/gene="nfi"
/locus_tag="DW48_RS0110245"
CDS 21499..22170
/gene="nfi"
/locus_tag="DW48_RS0110245"
/EC_number="3.1.21.7"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_007886280.1"
/GO_function="GO:0004519 - endonuclease activity [Evidence
IEA]"
/GO_process="GO:0006281 - DNA repair [Evidence IEA]"
/note="cleaves DNA at apurinic or apyrimidinic sites;
Derived by automated computational analysis using gene
prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="deoxyribonuclease V"
/protein_id="WP_025902268.1"
/translation="MADFALLKAEQQLLAAQVVREGLSPDFVPRFIAGGDTGFEENGT
VARAAIVILHWPSLEMAEHRVARVPVTLPYVPGYLSFRECPALLAAWQQLTHRPELMF
IDGHGVAHPRGLGLASHFGVTVDIPVIGVAKRRLCGDYQALSATPGDCQPLMYQQRQT
GWVLRSKARCNPLFISAGHRVSDDMALYWTRQCLRGYRLPEPTRLADAVASQRKGFLR
YLNRQ"
gene 22233..22823
/locus_tag="DW48_RS0110250"
CDS 22233..22823
/locus_tag="DW48_RS0110250"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_004206742.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="YjaG family protein"
/protein_id="WP_025902267.1"
/translation="MLRNPIHLRLEKLESWKHVTFMACLCERMYPNYQAFCQQTEFAD
AHIYRRILDLVWETLVVKDAKVNFDSQLEKLEEAIPDGDAFDFYGVYPAIDACVALSE
LLHAMLSGESLTYAIQVSETSVTTVAMLEMTQAGREMTDEELRENQAVIDEWDLQWEI
FRLLADCEERDLELIKGLRADLREAGISNIGISFNQ"
gene 23013..23285
/gene="hupA"
/locus_tag="DW48_RS0110255"
CDS 23013..23285
/gene="hupA"
/locus_tag="DW48_RS0110255"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_017460010.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="nucleoid-associated protein HU-alpha"
/protein_id="WP_007886275.1"
/translation="MNKTQLIDVIAEKADLSKTQAKTALESTLAAITESLKEGDAVQL
VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
gene 23463..24125
/locus_tag="DW48_RS0110260"
CDS 23463..24125
/locus_tag="DW48_RS0110260"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_003850138.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="DUF1481 domain-containing protein"
/protein_id="WP_025902266.1"
/translation="MMFSSGRGLLLCLALLLVGCQSAKNMPAFSASGYLSDRGVVRIW
RKNMPPDITIRTLYTPFDRTVTEQTDYHWQNGQLLMMKRHISGTSPDDVTLRFDNQGN
VSFMQRQLARQRESVSLQDIDLYRFDAGRLLQISNGLQAGRVTLMQGVWDGSNRVTTC
QHQTVSAGLDAYSVQMIYREQRQSAGAPVYIAWLRAPEGVQLLKATTENLCLQAPASA
DL"
gene complement(24201..25484)
/gene="purD"
/locus_tag="DW48_RS0110265"
CDS complement(24201..25484)
/gene="purD"
/locus_tag="DW48_RS0110265"
/EC_number="6.3.4.13"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_012882987.1"
/GO_function="GO:0004637 - phosphoribosylamine-glycine
ligase activity [Evidence IEA]"
/GO_process="GO:0006189 - 'de novo' IMP biosynthetic
process [Evidence IEA]; GO:0009152 - purine ribonucleotide
biosynthetic process [Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="phosphoribosylamine--glycine ligase"
/protein_id="WP_029990749.1"
/translation="MNILIIGNGGREHALAWKAAQSPLAGKVYVAPGNAGTALEPALH
NVDISATDIEGLLAFALKENIGLTIVGPEAPLVIGVVDAFREAGLKIFGPTRGAAQLE
GSKAFTKDFLARHNIPTAEYQNFTEIEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
LQEAEDAVQDMLAGNAFGDAGHRIVIEEFLQGEEASFIVMVDGEHVLPMATSQDHKRV
GDADTGPNTGGMGAYSPAPVVTDEVHQRAMDEVIWPTVRGMAAEGNVYTGFLYAGLMI
DPEGRPKVIEFNCRFGDPETQPIMLRLQSDLVALCLAACDGQLDQQESHWDPRPALGV
VLAAGGYPGKYRKGDVISGLDAATSPDGKVFHAGTTLQGEDVVTHGGRVLCATGLGTD
IAAAQKQAYALAKAISWEGSFCRTDIGYRAINRQK"
gene complement(25504..27093)
/gene="purH"
/locus_tag="DW48_RS0110270"
CDS complement(25504..27093)
/gene="purH"
/locus_tag="DW48_RS0110270"
/EC_number="2.1.2.3"
/EC_number="3.5.4.10"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_005132841.1"
/GO_function="GO:0003937 - IMP cyclohydrolase activity
[Evidence IEA]; GO:0004643 -
phosphoribosylaminoimidazolecarboxamide formyltransferase
activity [Evidence IEA]"
/GO_process="GO:0009152 - purine ribonucleotide
biosynthetic process [Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="bifunctional
phosphoribosylaminoimidazolecarboxamide
formyltransferase/IMP cyclohydrolase"
/protein_id="WP_029990751.1"
/translation="MQQRRPVRRALLSVSDKTGIVEFAKALTSRGVELLSTGGTARLL
ADAGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAVMAEHQIDPIDMVV
VNLYPFAETVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSHDYPALIREL
EENDNSLTLETRFDLAIKAFEHTAAYDSMIANYFGQRVPAYYSEDKTPAGRFPRTLNL
NFVKKQDMRYGENSHQDAAFYIEENIKEASVATAQQLQGKALSYNNIADTDAALECVK
EFPEPACVIVKHANPCGVAIGEDIKQAYERAYTTDPTSAFGGIIAFNRELDEATAQAI
ISRQFVEVIIAPSASEAALKITAAKQNVRVLTCGEWQQRESGLDFKRVNGGLLVQDRD
LGMVSEAQLRVVSQRQPTAQEMRDALFCWKVAKFVKSNAIVYARDNMTIGIGAGQMSR
VYSAKIAGIKAGDEGLEVKGSAMASDAFFPFRDGIDAAAAVGITCVIQPGGSIRDDEV
IAAADEHGIAMIFTDMRHFRH"
CONTIG join(ATMJ01000032.1:1..27620)
//