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Inhibition of Photorhabdus luminescens LecA expressed in Escherichia coli BL21(DE3) cells by fluorescence based analysis
Assay data:1 Tested
SummaryPubMed Citation
Displacement of [32P]NAD+ from Photorhabdus luminescens TccC3hvr ART domain assessed as inhibition of ADP-ribosylation of alpha-skeletal muscle actin at 10 to 100 uM preincubated for 15 mins followed by TccC3hvr addition and measured after 6 mins by SDS-PAGE based autoradiography
Displacement of [32P]NAD+ from Photorhabdus luminescens TccC3hvr ART domain assessed as inhibition of ADP-ribosylation of alpha-skeletal muscle actin at 100 uM preincubated for 15 mins followed by TccC3hvr addition and measured after 6 mins by SDS-PAGE based autoradiography
Displacement of [32P]NAD+ from Photorhabdus luminescens TccC3hvr ART domain assessed as inhibition of ADP-ribosylation of alpha-skeletal muscle actin preincubated for 15 mins followed by TccC3hvr addition and measured after 6 mins by SDS-PAGE based autoradiography
Induction of virulence of stlA deficient Photorhabdus luminescens BMM901 assessed as increase in proliferation of Photorhabdus luminescens BMM901 in Manduca sexta in presence of cinnamic acid
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed Citation
Induction of virulence of stlA deficient Photorhabdus luminescens BMM901 assessed as decrease in survival of Photorhabdus luminescens BMM901 infected Manduca sexta in presence of cinnamic acid
Induction of virulence of Photorhabdus luminescens TT01 assessed as increase in proliferation of Photorhabdus luminescens TT01 in Manduca sexta in presence of cinnamic acid
Induction of virulence of Photorhabdus luminescens TT01 assessed as decrease in survival of Photorhabdus luminescens TT01 infected Manduca sexta in presence of cinnamic acid
Induction of virulence of Photorhabdus luminescens TT01 assessed as increase in proliferation of Photorhabdus luminescens TT01 in Manduca sexta
Induction of virulence of Photorhabdus luminescens TT01 assessed as decrease in survival of Photorhabdus luminescens TT01 infected Manduca sexta
Antibacterial activity against Photorhabdus luminescens at 10 mg/ml after 24 hrs by disc diffusion method
Assay data:30 Tested
Summary
Antibacterial activity against Photorhabdus luminescens at 6.25 to 200 ug/ml after 24 hrs by microwell dilution method
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