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Inhibition of Zymomonas mobilis pyruvate decarboxylase assessed as inhibition constant preincubated for 60 mins followed by pyruvate addition using 10 uM ThDP as substrate by microplate reader analysis
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed Citation
Inhibition of Zymomonas mobilis pyruvate decarboxylase assessed as reduction in NADH consumption preincubated for 60 mins followed by pyruvate addition using 10 uM ThDP as substrate by microplate reader analysis
Assay data:2 Tested
SummaryPubMed Citation
Inhibition of Zymomonas mobilis pyruvate decarboxylase expressed in Escherichia coli preincubated for 3 mins followed by substrate addition using pyruvate as substrate by UV-visible spectrophotometeric analysis
Assay data:1 Active, 1 Activity ≤ 1 nM, 1 Activity ≤ 1 µM, 1 Tested
Ratio of Km for ThDP substrate activity at Zymomonas mobilis pyruvate decarboxylase to Ki for inhibition of Zymomonas mobilis pyruvate decarboxylase assessed as inhibition constant preincubated for 60 mins followed by pyruvate addition using 10 uM ThDP as substrate by microplate reader analysis
Inhibition of Zymomonas mobilis pyruvate decarboxylase incubated for 3 mins using pyruvate as substrate in presence of TPP by spectrophotometric analysis
Assay data:1 Active, 1 Activity ≤ 1 nM, 1 Activity ≤ 1 µM, 2 Tested
Inhibition of Zymomonas mobilis tRNA-guanine transglycosylase expressed in Escherichia coli cells BL21-CodonPlus (DE3)-RIPL in presence of and tRNATyr [-3H]-guanine incubated for 60 mins by liquid scintillation counting based radioactive assay
Assay data:3 Active, 2 Activity ≤ 1 µM, 13 Tested
Binding affinity to Zymomonas mobilis tRNA guanine transglycosylase by isothermal titration calorimetry
Binding affinity to Zymomonas mobilis tRNA guanine transglycosylase assessed as inhibition of [8-3H]guanine incorporation into Escherichia coli tRNA Tyr at position G34 preincubated for 10 mins followed by tRNA Tyr/[8-3H]guanine addition measured after 30 mins by liquid scintillation counting
Assay data:5 Active, 5 Activity ≤ 1 µM, 5 Tested
Binding affinity to Zymomonas mobilis TGT D156N mutant expressed in Escherichia coli Rosetta 2(DE3) by isothermal titration calorimetric analysis
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Binding affinity to Zymomonas mobilis TGT D102N mutant overexpressed in Escherichia coli BL21(DE3) by isothermal titration calorimetric analysis
Binding affinity to Zymomonas mobilis TGT E235Q mutant overexpressed in Escherichia coli BL21(DE3) by isothermal titration calorimetric analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed Citation
Binding affinity to wild-type Zymomonas mobilis TGT overexpressed in Escherichia coli BL21(DE3) by isothermal titration calorimetric analysis
Assay data:4 Active, 4 Activity ≤ 1 µM, 4 Tested
Inhibitory activity against eubacterial tRNA guanine transglycosylase (TGT) from Zymomonas mobilis
Assay data:10 Active, 4 Activity ≤ 1 µM, 17 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Selectivity ratio of Kd for Zymomonas mobilis TGT D102N mutant to Kd for wild-type Zymomonas mobilis TGT
Assay data:1 Tested
Selectivity ratio of Kd for Zymomonas mobilis TGT D156N mutant to Kd for wild-type Zymomonas mobilis TGT
Experimentally measured binding affinity data (Kd) for protein-ligand complexes derived from PDB
Assay data:3916 Active, 206 Activity ≤ 1 nM, 1830 Activity ≤ 1 µM, 3916 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
Experimentally measured binding affinity data (Ki) for protein-ligand complexes derived from PDB
Assay data:3598 Active, 421 Activity ≤ 1 nM, 2233 Activity ≤ 1 µM, 3598 Tested
Experimentally measured binding affinity data derived from PDB
Assay data:3073 Active, 3073 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
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