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RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
siRNA Circadian Assay
Assay data:527 Active, 33364 Tested
SummaryPubMed CitationRelated BioAssays by Depositor
An siRNA screen for human genes that are involved in human papilloma virus (HPV) E2 transcriptional repression of the E6 and E7 oncogenes
Assay data:497 Active, 21019 Tested
SummaryPubMed Citation
Genome-wide high-content siRNA screens for regulators of Parkin translocation in selective mitophagy (Dharmacon Library)
Assay data:826 Active, 18119 Tested
Genome-wide high-content siRNA screens for regulators of Parkin translocation in selective mitophagy (Ambion Library)
Assay data:9227 Active, 64752 Tested
RNAi screen for vorinostat resistance genes - Primary Screen
Assay data:450 Active, 18118 Tested
A whole genome RNAi screen highlights components of the ER/Golgi as a prominent source of resistance to immunotoxin-mediated cytotoxicity
Assay data:555 Active, 64752 Tested
InfectX: Pathogen infection siRNA screens
Assay data:115372 Tested
Identification of proteins required for the processing of the EWS-FLI1 nascent transcript
Assay data:59220 Tested
A screen to identify genes that regulate lymphatic endothelial cell migration - Primary Screen
Assay data:1034 Active, 18120 Tested
SummaryRelated BioAssays by Depositor
A screen to identify synthetic lethal interactions with E-cadherin in an MCF10A isogenic cell line
Assay data:2442 Active, 18052 Tested
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced TNF-alpha response in human macrophages_Primary screen TNF readout
Assay data:2853 Active, 18113 Tested
SummaryRelated BioAssays by Same Project
Binding affinity to recombinant human 6X His-tagged KCTD12 T1 domain (27 to 131 residues) expressed in Escherichia coli BL21 DE3 cells assessed as change in entropy by isothermal titration calorimetry
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to recombinant human 6X His-tagged KCTD12 T1 domain (27 to 131 residues) expressed in Escherichia coli BL21 DE3 cells preincubated for 1 hr followed by probing with HRP-conjugated 6X-His tag antibody for 0.5 hrs by SPOT peptide microarray analysis
Assay data:28 Active, 19 Activity ≤ 1 µM, 60 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Displacement of TAMRA-AAG-SLQLPILHHAYLPSIGGV from recombinant human 6X His-tagged KCTD12 T1 domain (27 to 131 residues) expressed in Escherichia coli BL21 DE3 cells by fluorescence polarization assay
Assay data:25 Active, 13 Activity ≤ 1 µM, 31 Tested
Binding affinity to recombinant human 6X His-tagged KCTD12 T1 domain (27 to 131 residues) expressed in Escherichia coli BL21 DE3 cells assessed as dissociation constant by isothermal titration calorimetry
Assay data:3 Active, 3 Activity ≤ 1 µM, 3 Tested
Potency index, ratio of Kd of RRLSLQLPILHHAYLPSI to Kd of compound for recombinant human 6X His-tagged KCTD12 T1 domain(27 to 131 residues) expressed in Escherichia coli BL21 DE3 cells by isothermal titration calorimetry
Assay data:1 Tested
Potency index, ratio of Kd of SLQLPILHHAYLPSIGGV to Kd of compound for recombinant human 6X His-tagged KCTD12 T1 domain (27 to 131 residues) expressed in Escherichia coli BL21 DE3 cells by isothermal titration calorimetry
Inhibition of interaction of N-terminal Myc tagged KCTD12 (unknown origin)/Myc-tagged human GABAB1b/rat GABAb2 transfected in HEK293 cells at 20 uM preincubated with KCTD12 for 4 hrs followed by GBR cell lysate addition and measured after 16 hrs by co-immunoprecipitation/immunoblot analysis
Inhibition of interaction of N-terminal Myc tagged KCTD12 (unknown origin)/Myc-tagged human GABAB1b/rat GABAb2 transfected in HEK293 cells at 200 uM preincubated with KCTD12 for 4 hrs followed by GBR cell lysate addition and measured after 16 hrs by co-immunoprecipitation/immunoblot analysis relative to control
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