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Inhibition of recombinant mouse SphK1 expressed in baculovirus infected Sf9 cells using D-erythro-sphingosine and [gamma-32P]ATP as substrate incubated for 30 mins by liquid scintillation counting analysis
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of recombinant mouse SphK1 transfected in HEK293T cells using D-erythro-sphingosine measured after 20 mins in presence of gamma-[p32]-ATP by autoradiography and liquid scintillation counting method
Assay data:1 Tested
Inhibition of recombinant mouse SphK1 expressed in baculovirus infected Sf9 insect cells using D-erythro-sphingosine as substrate measured after 30 mins in presence of [gamma32P]-ATP by liquid scintillation counting method
Inhibition of recombinant mouse SPHK1 using sphingosine as substrate in presence ATP
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of SphK1 in mouse erythrocytes using sphingosine as substrate by luminescence assay
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced NF-kappaB and TNF-alpha responses in mouse macrophages_Primary screen NFkB readout
Assay data:762 Active, 16821 Tested
SummaryRelated BioAssays by Same Project
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced NF-kappaB and TNF-alpha responses in mouse macrophages_Primary screen TNF readout
Assay data:1106 Active, 16821 Tested
A screen to identify the genes that modify the response of osteosarcoma to doxorubicin in mouse - Primary Screen
Assay data:788 Active, 16789 Tested
SummaryPubMed Citation
Inhibition of mouse SphK1
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Inhibition of mouse SPHK1 in mouse A20/2J cells using [3-3H]sphingosine as substrate assessed as [3H]S1P formation at 8 uM incubated for 5 mins prior to substrate addition measured after 5 mins
Inhibition of mouse SPHK1 in mouse A20/2J cells using [3-3H]sphingosine as substrate assessed as [3H]S1P formation at 2 to 4 uM incubated for 5 mins prior to substrate addition measured after 5 mins
Assay data:1 Active, 2 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Activity at mouse SPHK1 transfected in mouse A20/2J cell lysates assessed as compound phosphorylation at 10 uM after 30 mins by autoradiography in presence of [gamma-32P]ATP
Assay data:2 Active, 2 Tested
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