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Inhibition of patient derived UbcH5c in synovial fibroblast cells assessed as decrease in TNF- alpha induced IL-1 mRNA level preincubated for 2 hrs followed by TNF-alpha addition for 3 hrs by qPCR analysis
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
siRNA Circadian Assay
Assay data:527 Active, 33364 Tested
SummaryPubMed CitationRelated BioAssays by Depositor
An siRNA screen for human genes that are involved in human papilloma virus (HPV) E2 transcriptional repression of the E6 and E7 oncogenes
Assay data:497 Active, 21019 Tested
SummaryPubMed Citation
Genome-wide high-content siRNA screens for regulators of Parkin translocation in selective mitophagy (Dharmacon Library)
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Genome-wide high-content siRNA screens for regulators of Parkin translocation in selective mitophagy (Ambion Library)
Assay data:9227 Active, 64752 Tested
RNAi screen for vorinostat resistance genes - Primary Screen
Assay data:450 Active, 18118 Tested
A whole genome RNAi screen highlights components of the ER/Golgi as a prominent source of resistance to immunotoxin-mediated cytotoxicity
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InfectX: Pathogen infection siRNA screens
Assay data:115372 Tested
Identification of proteins required for the processing of the EWS-FLI1 nascent transcript
Assay data:59220 Tested
Summary
Immunoblotting assay for measuring pCHK1 signalling after ionising radiation
Assay data:20 Active, 42 Tested
Automated high-throughput/high-content microscopy assay for measuring IRIF kinetics
Assay data:13 Active, 41 Tested
A screen to identify genes that regulate lymphatic endothelial cell migration - Primary Screen
Assay data:1034 Active, 18120 Tested
SummaryRelated BioAssays by Depositor
A screen to identify synthetic lethal interactions with E-cadherin in an MCF10A isogenic cell line
Assay data:2442 Active, 18052 Tested
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced TNF-alpha response in human macrophages_Primary screen TNF readout
Assay data:2853 Active, 18113 Tested
SummaryRelated BioAssays by Same Project
Inhibition of UbcH5c (unknown origin) assessed as RNF4 ubiquitination at 2.5 mM after 15 mins in presence of fluorescently-labeled Ub/E2 by fluorescence assay
Assay data:3 Tested
Inhibition of UbcH5c (unknown origin) expressed in human 293T cells assessed as decrease in TNF-alpha-mediated NF-kB activation at 5 uM preincubated for 2 hrs followed by TNF-alpha stimulation for 8 hrs by luciferase reporter gene assay relative to control
Assay data:14 Tested
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Inhibition of patient derived UbcH5c in synovial fibroblast cells assessed as decrease in TNF- alpha induced MCP-1 mRNA level preincubated for 2 hrs followed by TNF-alpha addition for 3 hrs by qPCR analysis
Inhibition of patient derived UbcH5c in synovial fibroblast cells assessed as decrease in TNF- alpha induced MMP-3 mRNA level preincubated for 2 hrs followed by TNF-alpha addition for 3 hrs by qPCR analysis
Inhibition of UbcH5c in human HeLa cells assessed as TNF-alpha induced linear polyubiquitination of NEMO preincubated for 2 hrs followed by TNF-alpha addition for 5 mins by Western blot analysis
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