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Inhibition of PCAF (unknown origin) using histone H3/[acetyl-3H]-acetyl coenzyme A as substrate at 100 uM
Assay data:29 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of full length recombinant human FLAG-tagged PCAF expressed in baculovirus infected Sf21 cells using N-terminal H3 peptide substrate after 10 mins by radiometric filter binding assay in presence of [3H]acetyl-CoA
Assay data:2 Active, 2 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of full length recombinant human FLAG-tagged PCAF expressed in baculovirus expression system using histone substrate incubated for 10 mins by radiometric gel assay
Assay data:2 Active, 2 Activity ≤ 1 µM, 3 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of recombinant human PCAF catalytic domain (492 to 658 residues) using histone H3 peptide substrate at 100 uM after 1 hr by liquid scintillation counting method in presence of [3H]acetyl-CoA relative to control
Assay data:1 Tested
Inhibition of recombinant human PCAF expressed in bacterial system assessed as residual activity at 1 uM by bromoscan assay relative to control
Binding affinity to biotinylated PCAF (unknown origin) expressed in Escherichia coli at 0.2 to 1 uM by Biolayer Interferometric analysis
Assay data:2 Tested
Inhibition of PCAF bromodomain (719 to 832 amino acid residues) (unknown origin) expressed in Escherichia coli BL21(DE3) cells by NMR analysis
SummaryCompounds, ActiveRelated BioAssays by Target
Inhibition of recombinant human KAT2B catalytic domain using biotinylated histone H3 (1 to 21 amino acid residues) as substrate preincubated for 10 mins followed by acetyl-CoA addition measured after 10 mins by time resolved fluorescence assay
Assay data:13 Active, 22 Tested
Inhibition of recombinant human KAT2B catalytic domain using biotinylated histone H3 (1 to 21 amino acid residues) as substrate at 50 uM preincubated for 10 mins followed by acetyl-CoA addition measured after 10 mins by time resolved fluorescence assay
Assay data:28 Tested
SummaryRelated BioAssays by Target
Binding affinity to KAT2B in human HUT78 cells incubated for 45 mins by mass spectrometry based bromosphere chemoproteomic assay
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced TNF-alpha response in human macrophages_Primary screen TNF readout
Assay data:2853 Active, 18113 Tested
SummaryRelated BioAssays by Same Project
Inhibition of recombinant PCAF (unknown origin)
Inhibition of PCAF (unknown origin) using histone H3/[acetyl-3H]-acetyl coenzyme A as substrate
Inhibition of PCAF (unknown origin) using histone H3/[acetyl-3H]-acetyl coenzyme A as substrate at 10 uM
Inhibition of recombinant human PCAF catalytic domain (492 to 658 residues) using histone H3 peptide substrate after 1 hr by liquid scintillation counting method in presence of [3H]acetyl-CoA
Inhibition of human PCAF by BROMOscan assay
Inhibition of FLAG epitope-tagged recombinant human PCAF expressed in baculovirus infected insect Sf21 cells using [3H]acetyl-CoA as substrate preincubated for 10 mins followed by substrate addition measured after 10 mins by liquid scintillation counting analysis
Competitive inhibition of recombinant full-length human PCAF catalytic domain/GCN5 expressed in Escherichia coli BL21(DE3) using [14C]-acetyl CoA as substrate after 1 to 10 mins by double reciprocal plot analysis
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
A screen to identify synthetic lethal interactions with E-cadherin in an MCF10A isogenic cell line
Assay data:2442 Active, 18052 Tested
SummaryPubMed Citation
A screen to identify genes that regulate lymphatic endothelial cell migration - Primary Screen
Assay data:1034 Active, 18120 Tested
SummaryRelated BioAssays by Depositor
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