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Induction of Escherichia coli MurA-mediated 2-bromo-5-(2-nitrovinyl)furan formation assessed as enzyme-(R)-methyl 3-mercapto-2-(6-methoxy-2-oxo-2H-chromene-3-carboxamido)propanoate adduct level at 200 uM after 1 hr by SDS-PAGE analysis in presence of thiol nucleophile DTT in reducing laemmli buffer
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of Escherichia coli MurA C115D mutant expressed in Escherichia coli BL21(lambdaDE3) using UNAG and PEP as substrate incubated for 10 mins prior to PEP addition measured after 60 mins by spectrophotometric analysis
Assay data:1 Active, 7 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of Escherichia coli MurA expressed in Escherichia coli BL21(lambdaDE3) using UNAG and PEP as substrate at 5 uM incubated for 10 mins prior to UNAG and PEP addition measured after 60 mins by spectrophotometric analysis
Assay data:1 Active, 1 Tested
Inhibition of Escherichia coli MurA expressed in Escherichia coli BL21(lambdaDE3) using UNAG and PEP as substrate at 200 nM incubated for 10 mins prior to UNAG and PEP addition measured after 60 mins by spectrophotometric analysis
Irreversible inhibition of Escherichia coli MurA expressed in Escherichia coli BL21(lambdaDE3) using UNAG and PEP as substrate at 1.25 uM incubated up to 80 min prior to PEP addition measured after 60 mins by spectrophotometric analysis
Irreversible inhibition of Escherichia coli MurA expressed in Escherichia coli BL21(lambdaDE3) using UNAG and PEP as substrate at 100 nM incubated up to 80 min prior to PEP addition measured after 60 mins by spectrophotometric analysis
Inhibition of Escherichia coli MurA expressed in Escherichia coli BL21(lambdaDE3) using UNAG and PEP as substrate at 50 to 500 uM incubated for 30 mins by 2,2'-dithiopyridine based spectrophotometric analysis
Inhibition of Escherichia coli MurA C115D mutant expressed in Escherichia coli BL21(lambdaDE3) using UNAG and PEP as substrate at 50 to 500 uM incubated for 30 mins by 2,2'-dithiopyridine based spectrophotometric analysis
Assay data:1 Active, 2 Tested
Prodrug conversion assessed as Escherichia coli MurA-mediated 2-bromo-5-(2-nitrovinyl)furan formation at 100 uM by HPLC-UV analysis
Induction of Escherichia coli MurA-mediated 2-bromo-5-(2-nitrovinyl)furan formation assessed as enzyme-(R)-methyl 3-mercapto-2-(6-methoxy-2-oxo-2H-chromene-3-carboxamido)propanoate adduct level at 200 to 300 uM after 1 hr by SDS-PAGE analysis
Inhibition of Escherichia coli MurA expressed in Escherichia coli BL21(lambdaDE3) using UNAG and PEP as substrate incubated for 10 mins prior to PEP addition measured after 60 mins by spectrophotometric analysis
Assay data:3 Active, 1 Activity ≤ 1 µM, 7 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of IPTG-stimulated Escherichia coli K-12 murA at 4 fold MIC by microarray analysis
Assay data:2 Active, 2 Tested
Inhibition of IPTG-stimulated Escherichia coli K-12 murA at 8 fold MIC by microarray analysis
Inhibition of IPTG-stimulated Escherichia coli K-12 murA at 16 fold MIC by microarray analysis
Inhibition of Escherichia coli MurA C115D mutant
Assay data:2 Tested
Inhibition of Escherichia coli K12 Mur A C115D at 50 uM in presence of UNAG
Assay data:9 Tested
Inhibition of Escherichia coli K12 Mur A in presence of UNAG
Assay data:1 Active, 1 Activity ≤ 1 µM, 9 Tested
Inhibitory activity against MurA in Escherichia coli
Assay data:27 Tested
Inhibitory concentration against MurA enzyme in Escherichia coli
Assay data:4 Active, 28 Tested
Inhibition of recombinant Escherichia coli MurA assessed as residual activity at 500 uM using UDP-N-acetylglucosamine as substrate preincubated for 30 mins in presence of 1 mM DTT followed by substrate addition and measured by malachite green based colorimetric assay relative to control
Assay data:49 Tested
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