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Binding affinity to Escherichia coli ribosomal pre-translocation complex assessed as stabilization of hybrid-state occupancy by tRNA at 100 nM
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Activity of Escherichia coli ribosomal peptidyl transferase using Ac[14C]phe-tRNA as donor substrate assessed as [14C]polyphenylalanine formation after 40 mins by double reciprocal plot analysis in presence of poly(U)
Assay data:8 Tested
Binding affinity to Escherichia coli ribosome assessed as inhibition of transcription-translation activity preincubated for 30 mins followed by substrate addition measured after 5 mins by luciferase assay
Assay data:1 Active, 25 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of Escherichia coli ribosome-mediated translation after 60 mins by transcription/translation-coupled luciferase reporter gene assay
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of Ptase activity of Escherichia coli K-12 70S ribosome
Inhibition of Escherichia coli K-12 70S ribosome dependent elongation factor G-dependent translocation
Inhibition of tRNA binding to Escherichia coli K-12 70S ribosome
Inhibition of poly(A)-dependent synthesis of poly(Lys) using Escherichia coli K-12 70S ribosome at 20 uM after 1 hr by liquid scintillation counting
Assay data:2 Active, 2 Tested
Inhibition of poly(U)-dependent synthesis of poly(Phe) using Escherichia coli K-12 70S ribosome at 20 uM after 1 hr by liquid scintillation counting
Assay data:2 Tested
Displacement of [14C]-erythromycin from Escherichia coli K-12 70S ribosome after 10 mins by competitive binding assay
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Binding affinity to Escherichia coli ribosome assessed as inhibition of transcription-translational activity by luciferase assay
Assay data:2 Active, 1 Activity ≤ 1 µM, 9 Tested
Displacement of [3H]puromycin from 70S ribosomal complex in Staphylococcus aureus ATCC 29213 by competitive binding assay
Assay data:2 Active, 1 Activity ≤ 1 µM, 3 Tested
Displacement of [3H]chloramphenicol from 70S ribosomal complex in Staphylococcus aureus ATCC 29213 by competitive binding assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 6 Tested
Binding affinity to Escherichia coli ribosomal pre-translocation complex assessed as stabilization of hybrid-state occupancy by tRNA at 2 uM
Assay data:1 Active, 1 Tested
Binding affinity to Escherichia coli ribosomal pre-translocation complex assessed as decreased rate of EF-G-catalyzed translocation fraction by tRNA at 100 nM
Binding affinity to Escherichia coli ribosomal pre-translocation complex assessed as decreased rate of EF-G-catalyzed translocation by tRNA at 100 nM
Activity of Escherichia coli ribosomal peptidyl transferase using Ac[14C]phe-tRNA as donor substrate assessed as [14C]polyphenylalanine formation at 2 x 10'-4 M after 40 mins by double reciprocal plot analysis in presence of poly(U)
Binding affinity to Escherichia coli ribosomal pre-translocation complex assessed as decreased rate of EF-G-catalyzed translocation by tRNA at <2 uM
Binding affinity to Escherichia coli ribosomal pre-translocation complex assessed as stabilization of classical-state occupancy by tRNA at 200 uM
Binding affinity to Escherichia coli ribosomal pre-translocation complex assessed as stabilization of hybrid-state occupancy by tRNA at <2 uM
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