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Inhibition of His-tagged human recombinant ME3 (26 to 604 residues) using L-malate and beta-NADP+ as substrate at 0.1 uM preincubated with enzyme for 5 mins followed by beta-NADP+ challenge and incubated under shaking for 5 mins followed by incubation for 3 hrs without shaking by resorufin dye based fluorescence analysis
Assay data:5 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to ME3 in human BXPC-3 cell lysates assessed as change in melting temperature at 100 uM preincubated with cell lysates for 2 hrs followed by exposure to varying temperature by CETSA analysis
Assay data:1 Tested
Uncompetitive inhibition of His-tagged human recombinant ME3 (26 to 604 residues) using varying concentrations of beta-NADP+ as substrate in presence of fixed concentration of L-malate and measured for 10 mins by Lineweaver-Burk plot analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to ME3 in human BXPC-3 live cells assessed as change in melting temperature at 100 uM preincubated with live cells for 24 hrs followed by exposure to varying temperature by CETSA analysis
Competitive inhibition of His-tagged human recombinant ME3 (26 to 604 residues) using varying concentrations of L-malate as substrate in presence of fixed concentration of beta-NADP+ and measured for 10 mins by Lineweaver-Burk plot analysis
Inhibition of His-tagged human recombinant ME3 (26 to 604 residues) using L-malate and beta-NADP+ as substrate at 10 uM preincubated with enzyme for 5 mins followed by beta-NADP+ challenge and incubated under shaking for 5 mins followed by incubation for 3 hrs without shaking by resorufin dye based fluorescence analysis
Assay data:11 Tested
Inhibition of His-tagged human recombinant ME3 (26 to 604 residues) using L-malate and beta-NADP+ as substrate at 1 uM preincubated with enzyme for 5 mins followed by beta-NADP+ challenge and incubated under shaking for 5 mins followed by incubation for 3 hrs without shaking by resorufin dye based fluorescence analysis
Assay data:16 Tested
Inhibition of His-tagged human recombinant full length ME1 using L-malate and beta-NADP+ as substrate preincubated with enzyme for 5 mins followed by beta-NADP+ challenge and incubated under shaking for 5 mins followed by incubation for 2 hrs without shaking by resorufin dye based fluorescence analysis
Assay data:27 Active, 14 Activity ≤ 1 µM, 32 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of His-tagged human recombinant ME3 (26 to 604 residues) using L-malate and beta-NADP+ as substrate preincubated with enzyme for 5 mins followed by beta-NADP+ challenge and incubated under shaking for 5 mins followed by incubation for 3 hrs without shaking by resorufin dye based fluorescence analysis
Assay data:30 Active, 28 Activity ≤ 1 µM, 34 Tested
Activation of human recombinant wild type mitochondrial NADP-ME expressed in Escherichia coli BL21 assessed as one-half maximal activation by spectrophotometry
Assay data:2 Tested
Inhibition of wild type human recombinant mitochondrial NADP-ME expressed in Escherichia coli BL21 assessed as residual enzyme activity by spectrophotometry in presence of fumarate
Assay data:1 Active, 3 Tested
Inhibition of wild type human recombinant mitochondrial NADP-ME expressed in Escherichia coli BL21 assessed as residual enzyme activity by spectrophotometry in absence of fumarate
Assay data:3 Active, 3 Tested
Activation of human recombinant mitochondrial NADP-ME expressed in Escherichia coli BL21 assessed as residual enzyme activity by spectrophotometry in absence of fumarate
Activation of human recombinant mitochondrial NADP-ME expressed in Escherichia coli BL21 assessed as residual enzyme activity by spectrophotometry in presence of fumarate
Inhibitory activity against cytosolic malic enzyme
Assay data:6 Active, 3 Activity ≤ 1 µM, 10 Tested
Effect on rat hepatic malic enzyme(ME) activity 3 days after injection of 10e-6 mol/kg.
Effect on rat hepatic Malic enzyme activity relative to L-T3.
Effect on rat hepatic Malic enzyme activity 3 days after injection of 10e-6 mol/kg.
Assay data:4 Tested
Effect on rat hepatic Malic enzyme activity 3 days after injection of 10e-5 mol/kg.
Effect on rat hepatic malic enzyme(ME) activity relative to L-T3.
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