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Binding affinity to mouse 5-F-Trp-SPSB2 assessed as induction of sharpening of Trp207 resonance by measuring increase in peak height at protein to compound molar ratio of 1:5 by 19F-NMR spectroscopy
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to mouse 5-F-Trp-SPSB2 assessed as downward shift of Trp207 residue resonance at protein to compound molar ratio of 1:5 by 19F-NMR spectroscopy
Assay data:4 Active, 5 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Ratio of Kd for recombinant GST-tagged mouse SPSB2 (12 to 224 residues) expressed in Escherichia coli BL21 (DE3) by ITC method to Kd for recombinant GST-tagged mouse SPSB2 (12 to 224 residues) expressed in Escherichia coli BL21 by SPR analysis
Potency index, ratio of Ac-DINNNNH2 Kd to compound Kd for recombinant GST-tagged mouse SPSB2 (12 to 224 residues) expressed in Escherichia coli BL21 (DE3) by SPR analysis
Binding affinity to recombinant GST-tagged mouse SPSB2 (12 to 224 residues) expressed in Escherichia coli BL21 (DE3) by ITC method
Assay data:5 Active, 5 Activity ≤ 1 µM, 5 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Binding affinity to recombinant GST-tagged mouse SPSB2 (12 to 224 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation rate by SPR analysis
Assay data:4 Tested
Binding affinity to recombinant GST-tagged mouse SPSB2 (12 to 224 residues) expressed in Escherichia coli BL21 (DE3) by SPR analysis
Assay data:4 Active, 4 Activity ≤ 1 µM, 4 Tested
Binding affinity to recombinant GST-tagged mouse SPSB2 (12 to 224 residues) expressed in Escherichia coli BL21 (DE3) assessed as association rate by SPR analysis
Binding affinity to SPSB2 (unknown origin) at 3mM by 19F NMR method
Assay data:1 Active, 1 Tested
Binding affinity to SPSB2 (unknown origin) at 200 uM by surface plasmon resonance method
Assay data:6 Active, 18 Tested
Displacement of full-length of iNOS from SPSB2 in LPS/IFNgamma-stimulated mouse BMDM cell lysate at 10 uM after 2 hrs by Western blotting analysis
Binding affinity to GST-tagged SPSB2 (unknown origin) expressed in Escherichia coli BL21 (DE3) by SPR assay
Assay data:3 Active, 3 Activity ≤ 1 µM, 3 Tested
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