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Inhibition of Escherichia coli UTI89 FimH by hemagglutination assay
Assay data:10 Active, 1 Activity ≤ 1 nM, 10 Activity ≤ 1 µM, 10 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Relative inhibitory potency, ratio of methyl alpha-D-mannose Kd to compound Kd for binding affinity to Escherichia coli FimH by ELISA assay
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Relative inhibitory potency, ratio of methyl alpha-D-mannose Kd to compound Kd for binding affinity to C-terminal His-tagged truncated Escherichia coli FimH by SPR analysis
Assay data:3 Tested
Binding affinity to Escherichia coli FimH assessed as dissociation constant by SPR analysis
Assay data:3 Active, 1 Activity ≤ 1 µM, 3 Tested
Antagonist activity at recombinant 6His-tagged thrombin cleavage site of FimH Lectin domain expressed in Escherichia coli HM125 assessed as dissociation constant incubated for 24 hrs in presence of FITC-labeled tracer by competitive fluorescence polarization assay
Assay data:25 Active, 26 Activity ≤ 1 µM, 26 Tested
Binding affinity to Escherichia coli full-length FimH by competitive fluorescence polarization assay
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
Binding affinity to Escherichia coli FmlH lectin domain
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Inhibition of Escherichia coli C-terminal 6x-His-tagged biotinylated FmlH lectin domain expressed in Escherichia coli periplasm assessed as reduction in binding to desialylated bovine submaxillary mucin after 1 hr by ELISA
Assay data:24 Active, 12 Activity ≤ 1 µM, 37 Tested
Inhibition of Escherichia coli UTI89 FimH assessed as reduction in enzyme-mediated guinea pig erythrocyte hemagglutination titer
Assay data:10 Active, 4 Activity ≤ 1 µM, 10 Tested
Inhibition of Escherichia coli UTI89 FimH-mediated biofilm formation after 48 hrs by crystal violet staining-based assay
Assay data:12 Active, 11 Activity ≤ 1 µM, 14 Tested
Binding affinity to Escherichia coli UTI89 FimH lectin domain at 100 uM after 30 mins by SYPRO Orange-staining-based differential scanning fluorimetry
Assay data:14 Tested
Inhibition of Escherichia coli UTI89 FimH assessed as decrease in hemagglutination of guinea pig red blood cells after 4 to 18 hrs
Assay data:19 Active, 15 Activity ≤ 1 µM, 24 Tested
Displacement of TM-PAA polymer from Escherichia coli FimH-CRD-Th-6-His expressed in Escherichia coli HM125 by competitive binding assay relative to heptyl alpha-D-mannopyranoside
Assay data:18 Tested
SummaryRelated BioAssays by Target
Displacement of TM-PAA polymer from Escherichia coli FimH-CRD-Th-6-His expressed in Escherichia coli HM125 at <1 uM by competitive binding assay
Assay data:6 Tested
Displacement of TM-PAA polymer from Escherichia coli FimH-CRD-Th-6-His expressed in Escherichia coli HM125 by competitive binding assay
Assay data:19 Active, 19 Activity ≤ 1 µM, 19 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Inhibition of Escherichia coli FimH relative to heptyl alpha-D-mannopyranoside
Inhibition of Escherichia coli FimH relative to methyl alpha-D-mannopyranoside
Assay data:5 Tested
Antagonist activity at Escherichia coli FimH assessed as inhibition of yeast agglutination
Binding affinity to Escherichia coli FimH
Assay data:5 Active, 1 Activity ≤ 1 nM, 3 Activity ≤ 1 µM, 5 Tested
Binding affinity to Escherichia coli J96 wild type FimH lectin domain expressed in Escherichia coli C43 (DE3) by direct ITC analysis
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