Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Selectivity interaction (GPCR panel (PDSP screen)) EUB0001079a HTR5A
Assay data:1 Tested
SummaryRelated BioAssays by Target
Selectivity interaction (GPCR panel (PDSP screen)) EUB0001072a HTR5A
Selectivity interaction (GPCR panel (PDSP screen)) EUB0000276b HTR5A
Activity against 5HT5A/H in the Eurofins SafetyScreen44 (BI) by Radioactive Assay (Old_Cerep_2)
PDSP Primary Binding target: HTR5A - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintilation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter.
Assay data:30 Active, 175 Tested
PDSP Secondary Binding target: HTR5A - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.
Assay data:28 Active, 14 Activity ≤ 1 µM, 30 Tested
Binding affinity to 5-HT5A (unknown origin) at 10 uM
Displacement of [3H] LSD from human recombinant 5-HT5A receptor expressed in CHO-K1 cells assessed as inhibition constant incubated for 60 mins by spectrophotometric analysis
Assay data:2 Active, 2 Tested
Displacement of [3H] LSD from human recombinant 5-HT5A receptor expressed in CHO-K1 cells incubated for 60 mins by spectrophotometric analysis
Displacement of [3H] LSD from human recombinant 5-HT5A receptor expressed in CHO-K1 cells at 10 uM incubated for 60 mins by spectrophotometric analysis relative to control
Assay data:2 Tested
Inhibition of [3H]LSD from binding to 5-HT5A receptor (unknown origin) at 10 uM relative to control
Inhibition of 5HT5A receptor (unknown origin) at 1 uM relative to control
Displacement of [3H]LSD from 5-HT5A receptor (unknown origin) assessed as inhibition binding at 10 uM relative to control
Binding affinity to 5-HT5A (unknown origin) assessed as inhibition constant
Assay data:1 Active, 1 Tested
Inhibition of 5-HT5A (unknown origin) at 10 uM relative to control
Binding affinity to 5-HT5A receptor (unknown origin) assessed as inhibition constant
Binding affinity towards human HTR5A in an in vitro assay with cellular components measured by membrane filtration
Assay data:14 Active, 5 Activity ≤ 1 µM, 75 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Binding affinity to 5-HT5AR (unknown origin) assessed as inhibition constant
Assay data:6 Active, 4 Activity ≤ 1 µM, 8 Tested
Inhibition of 5HT5A receptor (unknown origin) at 10 uM
SummaryPubMed CitationRelated BioAssays by Target
Radioligand Binding Assay from US Patent US11642336: "5-HT2A agonists for use in treatment of depression"
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Filters: Manage Filters
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on