Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Inhibition of human recombinant C-terminal FLAG-tagged SMS1 expressed in mammalian expression system using C14-phosphatidylcholineD72 and C17-ceramide pre-incubated for 60 mins followed by incubation with substrate for 30 mins measured by RapidFire/MS assay
Assay data:4 Active, 1 Activity ≤ 1 µM, 17 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of mouse recombinant C-terminal FLAG-tagged SMS2 expressed in mammalian expression system using C14-phosphatidylcholineD72 and C17-ceramide pre-incubated for 60 mins followed by incubation with substrate for 30 mins measured by RapidFire/MS assay
Assay data:15 Active, 14 Activity ≤ 1 µM, 17 Tested
Inhibition of human recombinant C-terminal FLAG-tagged SMS2 expressed in Freestyle293 cell membrane using C14-phosphatidylcholineD72 and C17-ceramide pre-incubated for 60 mins followed by incubation with substrate for 30 mins measured by RapidFire/MS assay
Assay data:16 Active, 14 Activity ≤ 1 µM, 17 Tested
Inhibition of SMS2 in C57BL/6J mouse bone marrow derived macrophages after 17 hrs
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of SMS2 in C57BL/6J mouse bone marrow derived macrophages assessed as increase in [3H]-cholesterol efflux after 17 hrs by liquid scintillation analysis
Inhibition of SMS2 in human HuH7 cells assessed as down regulation of apoB secretion incubated for 17 hrs followed by further incubation for 2 hrs in presence of [35S]-methionine
Inhibition of purified SMS1 (unknown origin) using C6-NBD-Ceramide and DMPC as substrates preincubated for 5 mins followed by substrate addition and measured after 30 mins by TLC or HPLC method
Inhibition of purified SMS2 (unknown origin) using C6-NBD-Ceramide and DMPC as substrates preincubated for 5 mins followed by substrate addition and measured after 30 mins by TLC or HPLC method
Assay data:1 Active, 1 Activity ≤ 1 µM, 2 Tested
Inhibition of SMS2 (unknown origin) over-expressed in H5 insect cell lysate using C6-NBD-Ceramide and DMPC as substrates preincubated for 30 mins followed by substrate addition and measured after 2 hrs by TLC or HPLC method
Assay data:25 Active, 11 Activity ≤ 1 µM, 39 Tested
Inhibition of SMS2 (unknown origin) over-expressed in H5 insect cell lysate at 100 uM using C6-NBD-Ceramide and DMPC as substrates preincubated for 30 mins followed by substrate addition and measured after 2 hrs by TLC or HPLC method relative to control
Assay data:6 Tested
Inhibition of SMS2 in HFD-induced obese C57BL/6J mouse liver tissue lysate using NBD-ceramide as substrate assessed as reduction in DAG synthesis preincubated for 30 mins followed by substrate addition and measured after 3 hrs by HPLC analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of SMS1 in HFD-induced obese C57BL/6J mouse liver tissue lysate using NBD-phosphatidylcholine as substrate assessed as reduction in SM synthesis preincubated for 30 mins followed by substrate addition and measured after 3 hrs by HPLC analysis
Inhibition of SMS2 in MEF cells using C6-NBD-ceramide as substrate preincubated for 1 hr followed by substrate addition and measured after 1 hr by HPLC analysis
Inhibition of SMS1 in MEF cells using C6-NBD-ceramide as substrate preincubated for 1 hr followed by substrate addition and measured after 1 hr by HPLC analysis
Noncompetitive inhibition of SMS2 (unknown origin) stably expressed in mouse ZS cells using 5 to 50 uM C6-NBD-ceramide as substrate preincubated for 30 mins followed by substrate addition and measured after 3 hrs by HPLC analysis
Assay data:3 Active, 3 Activity ≤ 1 µM, 3 Tested
Noncompetitive inhibition of SMS1 (unknown origin) stably expressed in mouse ZS cells using 5 to 50 uM C6-NBD-ceramide as substrate preincubated for 30 mins followed by substrate addition and measured after 3 hrs by HPLC analysis
Assay data:3 Active, 3 Tested
Inhibition of SMS2 (unknown origin) stably expressed in mouse ZS cells using C6-NBD-ceramide as substrate preincubated for 30 mins followed by substrate addition and measured after 3 hrs by HPLC analysis
Assay data:4 Active, 4 Tested
Inhibition of SMS1 (unknown origin) stably expressed in mouse ZS cells using C6-NBD-ceramide as substrate preincubated for 30 mins followed by substrate addition and measured after 3 hrs by HPLC analysis
Inhibition of SMS2 (unknown origin)
Inhibition of SMS1 (unknown origin)
Filters: Manage Filters
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on