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Inhibition of human SPPL2A expressed in human U2OS cells assessed as nuclear mask transferring to EGFP channel using EGFP-labeled TNFalpha (aa1-76) NTF as substrate and measured after 24 hrs by Hoechst staining based translocation assay
Assay data:6 Active, 6 Activity ≤ 1 nM, 6 Activity ≤ 1 µM, 6 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of SPPL2a in mouse whole blood assessed as reduction in CD74/p8 NTF accumulation at 4 uM incubated for 5 hrs by flow cytometric analysis based assay
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of mouse SPPL2a cotransfected with EGFP-NLP-fused TNFalpha (1 to 77 residues)-NTF incubated for 24 hrs by Hoechst staining based Cellomics ArrayScan analysis
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Inhibition of SPPL2a in human U2OS cells cotransfected with EGFP-labeled TNFalpha (1 to 76 residues)-NTF incubated for 24 hrs by Hoechst staining based Cellomics ArrayScan analysis
Inhibition of SPPL2a in Balb/c mouse A20 cells assessed as reduction in CD74/p8 NTF accumulation incubated for overnight by Western blot analysis
Inhibition of human SPPL2a expressed in HEK293 cells cotransfected with Gal4 luciferase reporter plasmid and VP16-TNFalpha(1-76)-NTF incubated for 24 hrs by luciferase reporter gene assay based luminescence assay
Assay data:15 Active, 13 Activity ≤ 1 µM, 15 Tested
Inhibition of mouse SPPL2a in A20 cells assessed as decrease of CD74/p8 accumulation at 2 uM preincubated for 16 hrs followed by compound wash measured after 2 hrs by Western blot method
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of mouse SPPL2a in A20 cells assessed as decrease of CD74/p8 accumulation at 2 uM preincubated for 16 hrs followed by compound wash measured after 50 mins by Western blot method
Inhibition of mouse SPPL2a expressed in human U2OS cells using EGFP-labeled TNFalpha (1 to 77 residues) NLS as substrate after 24 hrs by Hoechst staining based high content imaging assay
Inhibition of human SPPL2a expressed in human U2OS cells using EGFP-labeled TNFalpha (1 to 76 residues) NTF as substrate after 24 hrs by Hoechst staining based high content imaging analysis
Inhibition of human SPPL2a expressed in HEK293 cells using GAL4-VP16 fusedTNFalpha (1 to 76)-NTF as substrate after 24 hrs by Bright-Glo luciferase reporter gene assay
Assay data:13 Active, 10 Activity ≤ 1 µM, 13 Tested
Inhibition of SPPL2a in mouse whole blood assessed as increase in CD74/p8 accumulation after 5.5 hrs by Western bot method
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Reversible inhibition of SPPL2a in mouse A20 cells assessed as increase in CD74/p8 accumulation at 2 uM preincubated for 16 hrs followed by compound wash out measured up to 2 hrs by Western blot method
Inhibition of mouse SPPL2a in A20 cells assessed as CD74/p8 accumulation by flow cytometry
Assay data:5 Active, 5 Activity ≤ 1 µM, 7 Tested
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