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Inhibition of C-terminal 6His-tagged recombinant Staphylococcus aureus SrtA with 1 to 59 residues truncated from N-terminus expressed in Escherichia coli BL21 (DE3) using dabcyl-QALPETGEE-edans as substrate preincubated for 1 hr followed by substrate addition and measured for 1 hr by FRET assay
Assay data:8 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of Staphylococcus aureus SrtA with 24 residues truncated from N-terminus using Abz-LPATG-Dap as substrate
Assay data:1 Active, 3 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of Staphylococcus aureus SrtA (60 to 206 residues) using Abz-LPETG-Dap(Dnp)-NH2 as substrate incubated for 1 hr by FRET assay
Assay data:3 Tested
Binding affinity to Staphylococcus aureus SrtA assessed as kinact/ki
Assay data:15 Tested
Inhibition of Staphylococcus aureus SrtA catalytic domain (60 to 206 residues) using Abz-LPETGDap(Dnp)-NH2 as substrate preincubated for 1 hr followed by substrate addition and measured immediately by FRET assay
Assay data:7 Active, 7 Activity ≤ 1 µM, 8 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of Staphylococcus aureus SrtA expressed in Escherichia coli BL21 (DE3) cells using 2-aminobenzoyl-LPETG-diaminopropionic acid-dinitrophenyl-NH2 as substrate preincubated for 1 hr followed by substrate addition and measured after 15 mins by fluorescence based analysis
Assay data:2 Tested
Inhibition of Staphylococcus aureus SrtA expressed in Escherichia coli BL21 (DE3) cells using Abz-LPETG-Dap(Dnp) as substrate assessed as kinact/ki
Inhibition of Staphylococcus aureus SrtA expressed in Escherichia coli BL21 (DE3) cells using Abz-LPETG-Dap(Dnp) as substrate preincubated for 30 mins followed by substrate addition and further incubated for 30 mins by HPLC analysis
Inhibition of Staphylococcus aureus USA 300 SrtA expressed in Escherichia coli BL21 (DE3) cells using Dabcyl-QALPETGEE-Edans as substrate preincubated for 30 mins followed by substrate addition and further incubated for 1 hr by FRET assay
Assay data:1 Tested
Inhibition of wild type recombinant Staphylococcus aureus ATCC 25904 Sortase A expressed in Escherichia coli BL21 using Dabcyl-QALPETGEE-Edans as substrate incubated for 30 mins followed by substrate addition and measured after 60 mins by FRET assay
Binding affinity to Staphylococcus aureus SrtA assessed as inhibition constant
Assay data:1 Active, 1 Tested
Binding affinity to Staphylococcus aureus SrtA using Abz-DNP as substrate assessed as second order rate constant of inhibition, kinact/ki
Binding affinity to Staphylococcus aureus SrtA assessed as first order rate constant of inactivation
Inhibition of Staphylococcus aureus SrtA transpeptidation activity
Inhibition of Staphylococcus aureus SrtA using FRET substrate incubated for 1 hr by FRET assay
Inhibition of Staphylococcus aureus SrtA using Abe-DNP as substrate by FRET assay
Inhibition of Staphylococcus aureus USA 300 SrtA expressed in Escherichia coli BL21 (DE3) cells using Dabcyl-QALPETGEE-Edans as substrate incubated for 1 hr by FRET assay
Inhibition of recombinant Staphylococcus aureus ATCC 6538p SrtA expressed in Escherichia coli TOP10 using Dabcyl-QALPETGEE-Edans as substrate incubated for 1 hr by fluorometric analysis
Assay data:1 Active, 13 Tested
Inhibition of recombinant Staphylococcus aureus SrtA using DabcylQALPETGEE-Edans as substrate by FRET assay
Assay data:4 Tested
Inhibition of Staphylococcus aureus ATCC 6538p SrtA using dabcyl-LPETG-edans as substrate incubated for 1 hr by fluorometric analysis
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