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Inhibition of Eu-W1024 streptavidin-labeled MAGEA4 (unknown origin) expressed in Escherichia coli BL21-(DE3) at 10 uM incubated for 30 to 60 mins by LANCE TR-FRET assay
Assay data:3 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain D115R mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry
Assay data:1 Tested
Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain R299A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry
Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain R297A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry
Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain V294A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry
Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain V291A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry
Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain M229A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry
Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain V228A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry
Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain E225A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry
Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain I205A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry
Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain M161A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry
Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as protection in trypsin-induced MAGEA4 protein degradation incubated for 1 hr by proteolysis based SDS-PAGE analysis
Assay data:2 Active, 2 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of Eu-W1024 streptavidin-labeled MAGEA4 (unknown origin) expressed in Escherichia coli BL21-(DE3) incubated for 30 to 60 mins by LANCE TR-FRET assay
Assay data:16 Active, 1 Activity ≤ 1 nM, 10 Activity ≤ 1 µM, 16 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain (unknown origin) expressed in Escherichia coli BL21-(DE3) by isothermal titration calorimetry
Assay data:3 Active, 3 Activity ≤ 1 µM, 3 Tested
Binding affinity to MAGEA4 (unknown origin) expressed in Escherichia coli BL21-(DE3) by isothermal titration calorimetry
Binding affinity to MAGEA4 in human NCI-H23 cells at 1 uM by mass spectrometry based pull down assay
Assay data:1 Active, 1 Tested
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