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Protac activity at CRBN/Sirt7 in human A549 cells assessed as reduction in Sirt7 protein level at 0.1 to 10 uM by Western blot analysis
Assay data:1 Tested
SummaryRelated BioAssays by Target
Inhibition of SIRT7 (unknown origin) at 10 uM relative to control
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of SIRT7 (unknown origin)
Inhibition of human recombinant SIRT7 at 10 uM incubated for 20 mins followed by mixing and measure after 30 mins by Luminescence assay relative to control
Assay data:8 Tested
Inhibition Assay from US Patent US9359293: "Methods of treatment using modulators of SIRT2"
Assay data:3 Active, 2 Activity ≤ 1 µM, 4 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Binding affinity to SIRT7 (unknown origin) assessed as increase in fluorescence intensity at 2.5 uM measured at 5 mins interval for 1 hr in presence of NAD+ by fluorometric method relative to control
Assay data:12 Tested
Substrate activity at human N-terminal His-tagged SIRT7 (68 to 354 residues) expressed in Escherichia coli at 50 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay
Assay data:16 Tested
Substrate activity at human N-terminal His-tagged SIRT7 (68 to 354 residues) expressed in Escherichia coli at 5 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay
Substrate activity at human N-terminal His-tagged SIRT7 (68 to 354 residues) expressed in Escherichia coli at 0.5 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay
Assay data:11 Tested
Inhibition of tRNA-activated His6-tagged human SIRT7 deacetylation activity using acetyl-lysine as substrate in presence of beta-NAD+ after 30 mins by reversed-phase HPLC analysis
Inhibition of tRNA-activated deacetylation activity of recombinant human full length N-terminal His6-tagged SIRT7 expressed in Escherichia coli using H2N-GGKAPR-[Nepsilon-acetyl-lysine]-QLATKA-CONH2 as substrate measured after 30 mins by HPLC analysis
Assay data:1 Active, 3 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Assay data:5 Tested
Inhibition of recombinant human N-terminal His6-tagged SIRT7 (68 to 354 residues) expressed in Escherichia coli Transetta(DE3) using AcIQF as substrate preincubated for 30 mins followed by substrate and NAD+ addition measured after 2 hrs by fluorescence assay
Activity of recombinant human SIRT7 assessed as deacylation of compound at 50 uM after 1 hr by trypsin developer-based fluorescence assay
Assay data:7 Tested
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